LOCUS       sequence-99958-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIbeta R E-F
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    20..44
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(130..435)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(232..251)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
       61 accagaggtc tcactcatac gaagtcttcg tcgacctgca gactggctgt gtataaggga
      121 gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg
      181 gtggctgaga tcagccactt cttccccgat aacggaaacc ggcacactgg ccatatcggt
      241 ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggggac
      301 tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc cgggcgtgtc
      361 aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta
      421 aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa
      481 taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt tcggcacgca
      541 gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg acatcatata
      601 tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct gcttcatagc
      661 atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat accgcaaaaa
      721 tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc tctagattac
      781 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
      841 aagccat
//
