LOCUS       sequence-99962-e        7094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAD18-cm encoding rbs33-hrpS-ter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Wang B, Kitney RI, Joly N, Buck M
  TITLE     Engineering modular and orthogonal genetic logic gates for robust 
            digital-like synthetic biology.
  JOURNAL   Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
  PUBMED    22009040
REFERENCE   2  (bases 1 to 7094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-10-18"; volume: "2:508. doi"; pages: " 
            10.1038/ncomms1516"
COMMENT     Sequence Label: pBW213ara-hrpS
FEATURES             Location/Qualifiers
     source          1..7094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1001..1285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1208..1227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      2244..2315
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2331..2358
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    2365..2385
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2463..2480)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2633..2719
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2811..2838
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2857..2948
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3133..3511
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(3167..3186)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(4174..4833)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4748..4767
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      5264..5719
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5351..5370)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5830..6418
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6319..6338
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6572..6589
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6604..6744
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6670..6689)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6830..6852
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6991..7010
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagagtcac acaggactac
     1321 tagatgagtc ttgatgaaag gtttgaggat gatctggacg aggagcgggt tccgaatctg
     1381 gggatagttg ccgaaagtat ttcgcaactg ggtatcgacg tgctgctatc gggtgagacc
     1441 ggcacgggca aagacacgat tgcccgacgg attcatgaga tgtcaggccg caaagggcgc
     1501 ctggtggcga tgaattgcgc ggccattccg gagtccctcg ccgagagcga gttattcggc
     1561 gtggtcagcg gtgcctacac cggcgctgat cgctccagag tcggttatgt cgaagcggcg
     1621 cagggcggca cgctgtacct ggatgagatc gatagcatgc cgctgagcct gcaagccaaa
     1681 ttgctgaggg tgctggaaac ccgagcgctt gaacggctgg gttcgacgtc gacgatcaag
     1741 ctggatatct gcgtgatcgc ctccgcccaa tgctcgctgg acgacgccgt cgagcggggg
     1801 cagtttcgtc gcgatctgta ttttcgcctg aacgtcctga cactcaagct tcctccgcta
     1861 cgtaaccagt ctgatcgcat agttcccctg ttcacacgtt ttacggccgc cgccgcgagg
     1921 gagctcggtg ttcccgttcc cgatgtttgc ccactgctgc acaaagtgct gctgggccac
     1981 gactggcccg gcaatatccg tgagctcaag gcggcagcca aacgccatgt gctgggtttc
     2041 cccttgctgg gcgccgagcc gcagggcgaa gagcacttgg cctgtgggct caaatcgcaa
     2101 ttgcgagtga tcgaaaaagc cctgattcag gagtcgctca agcgccacga caattgtgtg
     2161 gattcggtaa gcctggaact ggacgtgcca cgccgtacgc tctatcgacg catcaaagaa
     2221 ttgcagatct aataaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2281 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2341 cgggtgggcc tttctgcgtt tatatactag tagcggccgc tgcagggtac ccggggatcc
     2401 tctagagtcg acctgcaggc atgcaagctt ggctgttttg gcggatgaga gaagattttc
     2461 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
     2521 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
     2581 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
     2641 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2701 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     2761 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
     2821 cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt
     2881 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2941 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3001 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3061 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3121 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3181 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     3241 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     3301 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     3361 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     3421 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     3481 ccacgatgcc tgcagcaatg gcaacaacgt tgcgtgctgc tgaagttgcc cgcaacagag
     3541 agtggaacca accggtgata ccacgatact atgactgaga gtcaacgcca tgagcggcct
     3601 catttcttat tctgagttac aacagtccgc accgctgtcc ggtagctcct tccggtgggc
     3661 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     3721 acaggtgccg gcagcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     3781 gaaacaagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc
     3841 ctgtggaaca cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag
     3901 gcagaataaa tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg
     3961 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac
     4021 cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat
     4081 ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg tagcaccagg
     4141 cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca
     4201 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg
     4261 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     4321 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     4381 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     4441 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     4501 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     4561 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc
     4621 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     4681 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     4741 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     4801 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     4861 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     4921 tcttacgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4981 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5041 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5101 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5161 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5221 aactgtcaga ccaagtttac tcatatatac tttagattga tttacgcgcc ctgtagcggc
     5281 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5341 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5401 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5461 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5521 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5581 tgaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     5641 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5701 atattaacgt ttacaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5761 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5821 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5881 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5941 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6001 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6061 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6121 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6181 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6241 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6301 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6361 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6421 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6481 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6541 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6601 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     6661 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     6721 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     6781 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     6841 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagcaagg agatggcgcc
     6901 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     6961 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     7021 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctgctcatg
     7081 tttgacagct tatc
//
