LOCUS       sequence-99967-e        4913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBWF2620 encoding rbs33-hrpR-ter (pBWF2620: pSB3K3 carrying 
            BBa_F2620 (PTet-rbs34-luxR-ter)).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Wang B, Kitney RI, Joly N, Buck M
  TITLE     Engineering modular and orthogonal genetic logic gates for robust 
            digital-like synthetic biology.
  JOURNAL   Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
  PUBMED    22009040
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-10-18"; volume: "2:508. doi"; pages: " 
            10.1038/ncomms1516"
COMMENT     Sequence Label: pBW313lux-hrpR
FEATURES             Location/Qualifiers
     source          1..4913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(219..1034)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(219..1034)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     942..961
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1026..1045
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(1629..2173)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1709..1728)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2641..2659
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2663..2706)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2709..2730
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    2731..2749
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2756..2774
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2793..2804
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3608..3679
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3695..3722
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             4721..4732
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     terminator      4773..4844
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4860..4887
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    join(4894..4913,1)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gtccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
       61 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      121 gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg agtcccgtca agtcagcgta
      181 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
      241 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      301 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      361 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      421 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      481 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      541 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
      601 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      661 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      721 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      781 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
      841 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
      901 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
      961 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt
     1021 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1081 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     1141 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
     1201 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
     1261 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcc
     1321 tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgctagcg gagtgtatac
     1381 tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa
     1441 aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct
     1501 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg
     1561 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca
     1621 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
     1681 cagtggtggc gaaacccgac aggactataa agataccagg cgtttcccct ggcggctccc
     1741 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1801 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1861 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1921 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1981 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     2041 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     2101 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     2161 aaaacgatct caagaagatc atcttattaa ggggtctgac gctcagtgga acgaaaactc
     2221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2341 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2401 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2461 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2521 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2581 tattccatgg tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2641 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2701 gatttctgga attcgcggcc gcttctagag tccctatcag tgatagagat tgacatccct
     2761 atcagtgata gagatactga gcactactag agaaagagga gaaatactag atgaaaaaca
     2821 taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg
     2881 atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg
     2941 cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta
     3001 aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt
     3061 attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata
     3121 aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt
     3181 tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag
     3241 acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt
     3301 ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa
     3361 ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt
     3421 caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga
     3481 aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg
     3541 attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc
     3601 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     3661 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     3721 cgtttatata ctagagacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat
     3781 agtcgaataa atactagagt cacacaggac tactagatga gtacaggcat cgataaggac
     3841 gtccgagagt gttggggcgt aactgcatta tcagcgggtc atcaaattgc aatgaatagc
     3901 gcgtttctgg atatggactt gctgttgtgc ggggaaaccg gcaccggcaa ggacacactg
     3961 gccaaccgca ttcacgagtt gtccagcagg tcgggaccct ttgtgggcat gaactgcgcc
     4021 gccattcccg agtcgctggc agagagccag ttattcggtg tggtcaacgg tgcattcacc
     4081 ggcgtatgcc gggctcgcga gggctacata gaggcctcca gtggtggcac cttgtacctg
     4141 gatgaaatcg acagcatgcc gttgagcctg caagccaaac tgctgcgtgt gttggagagt
     4201 cgaggtatcg agcgtctggg ctcgaccgaa tttatcccgg tggatctgcg gatcattgcc
     4261 tcggcccagc ggccactgga tgaactggtg gaacaaggac ttttccgtcg cgacctgttt
     4321 tttcggctca acgtgctgac gcttcacttg ccagccttgc gcaaacgtcg tgaacagatc
     4381 ctgccattgt tcgaccagtt cacccagggt atcgctgccg agttcggacg tcccgctcct
     4441 gcgctggaca gcgggcgtgt gcagctgctg ctcagccacg actggccggg caacatccgc
     4501 gaattgaagt ctgcggccaa gcgcttcgta ctcggcttcc ccttgctggg cgccgaccct
     4561 gtggaagcgc ttgaccctgc cacggggctg cgcacgcaaa tgcgcatcat cgagaaaatg
     4621 ctcatccagg atgccttgaa gcggcacagg cacaatttcg acgcggtgct tcaggagttg
     4681 gagttgccaa gacgcaccct gtatcaccgc atgaaggaac tgggagttgc agcgccgatc
     4741 gctgcgacgg ccggggtcta ataaccaggc atcaaataaa acgaaaggct cagtcgaaag
     4801 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     4861 gctcaccttc gggtgggcct ttctgcgttt atatactagt agcggccgct gca
//
