LOCUS       sequence-99978-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila, backbone for donor plasmid assembly. CRISPR knock-in..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: pBS-GGAC-ATGC
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      119..575
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(206..225)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     416..437
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     701..723
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     715..732
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     716..732
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     742..761
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        742..760
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
       61 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
      121 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      181 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      241 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      301 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
      361 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
      421 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
      481 gggattttgc cgatttcggc ctattggtta aaaaaatgag ctgatttaac aaaaatttaa
      541 cgcgaatttt aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac
      601 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
      661 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
      721 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg tacggactga
      781 gacgtggtgt gcacaccacg tctcaatgca ggtggagctc cagc
//
