LOCUS       sequence-99979-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila, backbone for donor plasmid assembly. CRISPR knock-in..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: pBS-GGAC-TTCT
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(77..97)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(77..95)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(116..132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(116..132)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(129..151)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    140..156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(164..170,171..188,189..194))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    209..230
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(347..364)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(598..617)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggcgaattg ggtacggact gagacgtggt gtgcacacca cgtctcattc tgcaggtgga
       61 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
      121 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      181 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      241 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      301 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
      361 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      421 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      481 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      541 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      601 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      661 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      721 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaa
//
