LOCUS       sequence-99980-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila, backbone for donor plasmid assembly. CRISPR knock-in..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: pBS-GGAC-TTCT
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      98..554
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(185..204)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     395..416
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     680..702
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     694..711
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     695..711
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     721..740
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        721..739
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
       61 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
      121 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      181 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      241 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      301 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      361 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      421 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
      481 tattggttaa aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
      541 aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
      601 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
      661 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
      721 taatacgact cactataggg cgaattgggt acggactgag acgtggtgtg cacaccacgt
      781 ctcattctgc aggtggagct ccagcttttg ttccc
//
