LOCUS       sequence-99984-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, 
            flanked attP. section marker, assemble with homology arms to make 
            Donor plasmid for CRISPR. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: pJET1.2-STOP-dsRed
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..121
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     polyA_signal    253..387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(277..296)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     331..350
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtctcaccag tctcgaagct tcccaggtca gaagcggttt tcgggagtag tgccccaact
       61 ggggtaacct ttgagttctc tcagttgggg gcgtagggtc gccgacatga cacaaggggt
      121 tggatccccg ggagtcgatc caacatggcg acttgtccca tccccggcat gtttaaatat
      181 actaattatt cttgaactaa ttttaatcaa ccgatttatc tctcttccgc aggtctaact
      241 aactaacacc gcgatccaga catgataaga tacattgatg agtttggaca aaccacaact
      301 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
      361 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
      421 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
      481 gctgattatg atcaactagt ctggctcgcc cggggatcta attcaattag agactaattc
      541 aattagagct aattcaatta ggatccaagc ttatcgattt cgaaccctcg accgccggag
      601 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
      661 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatcggg
      721 gtaccgctag agtcgacggt accgc
//
