LOCUS       sequence-99985-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, 
            flanked attP. section marker, assemble with homology arms to make 
            Donor plasmid for CRISPR. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: pJET1.2-STOP-dsRed
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    163..244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(200..219)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    272..371
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
ORIGIN
        1 cagtacgagc gcaccgaggg ccgccaccac ctgttcctgt agcggcggcg actctagatc
       61 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      121 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      181 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      241 ctgccctagg catatgcggt gtgaaatatt caaccccttg tgtcatgtcg gcgaccctac
      301 gcccccaact gagagaactc aaaggttacc ccagttgggg cactactccc gaaaaccgct
      361 tctgacctgg gccgcggaac gagagactgt ttgagacgta tctttctaga agatctccta
      421 caatattctc agctgccatg gaaaatcgat gttcttcttt tattctctca agattttcag
      481 gctgtatatt aaaacttata ttaagaacta tgctaaccac ctcatcagga accgttgtag
      541 gtggcgtggg ttttcttggc aatcgactct catgaaaact acgagctaaa tactgatgaa
      601 tcccctaatg attttggtaa aaatcattaa gttaaggtgg atacacatct tgtcatatga
      661 tcaaatggtt tcgcgaaaaa tcaataatca gacaacaaga tgtgcgaact cgatatttta
      721 cacgactctc tttaccaatt ctgccccgaa ttacactt
//
