LOCUS       sequence-99986-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, 
            flanked attP. section marker, assemble with homology arms to make 
            Donor plasmid for CRISPR. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: pJET1.2-STOP-dsRed
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    7..141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(31..50)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     85..104
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      520..529
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 caccgcgatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
       61 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
      121 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
      181 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
      241 tatgatcaac tagtctggct cgcccgggga tctaattcaa ttagagacta attcaattag
      301 agctaattca attaggatcc aagcttatcg atttcgaacc ctcgaccgcc ggagtataaa
      361 tagaggcgct tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc
      421 taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat cggggtaccg
      481 ctagagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gcgctcctcc
      541 aagaacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggcac cgtgaacggc
      601 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcca caacaccgtg
      661 aagctgaa
//
