LOCUS       sequence-99996-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid
            of the attB site. used for RMCE. CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: p3xP3-EGFP.vas-int.NLS
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctggctaga actagtggat ccaacacgaa gagcagcagt gtggtggatt tctcagttat
       61 tgattttgct tctgcaaaga cagcaaacgc aaaagggcaa catttgctgg tcggcatgct
      121 tttcggggga ggaggactgg gcgcgagcag ggcgaggcat gcattcaaaa agccggctcc
      181 aacggagagc gagagagcgc aagagccaag tgtacagcaa ggcaaacaac aaacaacgaa
      241 acacgaagga tgagtactta aaaccaaaca cgtgatagta cacggtatta gttgggatca
      301 aagtgcacgt ttctgttaag ttttgcaacc gctcgttatt aaaagcagtt cacaaaatac
      361 ttaaaagaaa tcgaattaga gccagaacat tttttcaaat tatattttgt tatacatttc
      421 tttgtaaaag gaaattaaaa tagaaaattc gaatggcttg catatcctaa atatgcattt
      481 tattattata tgcgttataa tacatataat taatatgtta ttaatatcta acttatttaa
      541 ctaattacat caattactgc attacattta attatttgaa atctatgtcc atacattctc
      601 gagctacgcc gctacgtctt ccgtgccgtc ctgggcgtcg tcttcgtcgt cgtcggtcgg
      661 cggcttcgcc cacgtgatcg aagcgcgctt ctcgatgggc gttccctgcc ccctgcccgt
      721 agtcgacttc gtgacaacga tcttgtctac gaagagcccg acgaacacgc gcttgtcgtc
      781 tactgacgcg cg
//
