LOCUS       sequence-99999-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila, backbone for donor plasmid assembly. CRISPR knock-in. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: pBS-CGGA-GTGC
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      88..544
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(175..194)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     385..406
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     670..692
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     684..701
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     685..701
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     711..730
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        711..729
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
       61 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
      121 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      181 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      241 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      301 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
      361 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
      421 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
      481 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
      541 atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      601 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      661 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
      721 cactataggg cgaattgggt acggatgaga cgtggcacac cacgtctcag tgcaggtgga
      781 gctccagc
//
