This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
We have implemented updates to our Transparency and Privacy Policy in light of the new GDPR standards. When logging in or creating a new account, you will be asked to read and acknowledge these policy changes. Additionally, you can find our Transparency and Privacy Policy here.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Kim Lab CRISPR Plasmids Available from Addgene

A human codon-optimized Streptococcus pyogenes Cas9 nuclease under the control of the CMV promoter. The resulting Cas9 enzyme, when expressed with a gRNA, was shown to efficiently cleave two genomic sites and induce InDels with frequencies of up to 33% in a human cell line.

These plasmids are described in:

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Cho SW, Kim S, Kim JM, Kim JS. Nat Biotechnol .2013 Mar;31(3):230-2. doi: 10.1038/nbt.2507. PubMed .

Individual plasmids can be ordered via the links below:

ID Plasmid
43945 p3s-Cas9HC : A human codon-optimized Cas9 expression plasmid, for use with a chimeric gRNA in human cells. Add to Cart