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Marraffini Lab CRISPR Plasmids Available from Addgene

A two plasmid system for editing bacterial genomes using an RNA-guided Cas9 nuclease.

pCas9 cartoon pCRISPR cartoon


Icon Marraffini pCRISPR protocol (106.7 KB)

These plasmids are described in:

RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Jiang W, Bikard D, Cox D, Zhang F, Marraffini L. Nat Biotechnology . 2013 Jan 29. PubMed .

Individual plasmids can be ordered via the links below:

ID Plasmid
42876 pCas9 : A Cas9 nuclease and tracrRNA dual expression plasmid. Add to Cart
42875 pCRISPR : A crRNA expression plasmid for targeting a specific sequence. Add to Cart
44505 pCRISPR::rpsL : A crRNA expression plasmid specific to the rpsL allele. Add to Cart