Fluorescent Proteins: Subcellular Localization

Background

The subcellular localization of a protein is often tied to its function, so it's important to determine where your protein of interest resides. There are a variety of methods for determining subcellular localization. One of the most common is to use fluorescence microscopy to compare the localization of your protein of interest with known markers.

Addgene has assembled a collection of fluorescent fusion proteins that are targeted to various organelles or subcellular structures. You should fuse your protein of interest with a fluorescent protein that has a different emission wavelength. For example, if the marker protein is fused to GFP, you may want to fuse your protein to a red fluorescent protein (see this fusion protein guide to find the appropriate backbone). Then, by examining the overlap between your fusion protein and the marker fusion protein using fluorescence microscopy, you can assess whether your protein is targeted to the same structures as these well-characterized proteins.

General Structures

The cell contains a variety of organelles and subcellular structures each with their own unique function. The nucleus, for example, houses the cell’s genetic material. The mitochondria produces the energy cells required for chemical reactions. Labeling these structures can be quite useful for identifying the localization or possible function of a protein of interest. Browse or search the table below to find markers of general cellular organelles and structures.

	ID	Plasmid	Structure	Targeting Gene/Signal	Fluorescent Protein	PI
	98875	3xnls-mNeonGreen	Nucleus	3xNLS	mNeonGreen	Dorus Gadella
	98817	3xnls-mTurquoise2	Nucleus	3xNLS	mTurquoise2	Dorus Gadella
	98816	3xnls-mScarlet-I	Nucleus	3xNLS	mScarlet-I	Dorus Gadella
	98876	4xmts-mNeonGreen	Mitochondria	4xmts	mNeonGreen	Dorus Gadella
	98819	4xmt-mTurquoise2	Mitochondria	4xmts	mTurquoise2	Dorus Gadella
	98818	4xmts-mScarlet-I	Mitochondria	4xmts	mScarlet-I	Dorus Gadella
	19031	pABCb10-GFP	Mitochondria	ABCb10	GFP	Orian Shirihai
	158003	pCMV-mGold-CAF1-C-10	Nucleus	CAF-1	mGold	Francois St-Pierre
	27705	CAV1-mCherry	Caveolae	Cav1	mCherry	Ari Helenius
	37533	cfSGFP2-N	Extracellular milieu	cfSGFP2-N	EGFP	Ikuo Wada
	73208	pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3	Mitochondria	COX IV-derived (1-22 aa) import sequence and COX VIII signal peptide	dL5 FAP; mCerulean3	Marcel Bruchez
	44385	pLV-mitoGFP	Mitochondria	COX8	GFP	Pantelis Tsoulfas
	80000	pMito-miRFP703	Mitochondria	COX8A	miRFP703	Vladislav Verkhusha
	36208	pmTurquoise2-Mito	Mitochondria	COX8A(1-29)	mTurquoise2	Dorus Gadella
	26673	pEGFP-C1-Fibrillarin	Nucleolus	Fibrillarin	EGFP	Sui Huang
	36207	pmTurquoise2-H2A	Nucleus	H2A	mTurquoise2	Dorus Gadella
	85053	pmScarlet-i_H2A_C1	Nucleus	H2A	mScarlet-I	Dorus Gadella
	85052	pmScarlet-H_H2A_C1	Nucleus	H2A	mScarlet-H	Dorus Gadella
	85051	pmScarlet_H2A_C1	Nucleus	H2A	mScarlet	Dorus Gadella
	18982	pHIV-H2BmRFP	Chromatin	H2B	mRFP	Bryan Welm, Zena Werb
	21045	pH2B_mCherry_IRES_puro2	Chromatin	H2B	mCherry	Daniel Gerlich
	25999	LV-GFP	Chromatin	H2B	GFP	Elaine Fuchs
	11680	H2B-GFP	Chromatin	H2B	GFP	Geoff Wahl
	21210	PGK-H2BeGFP	Chromatin	H2B	EGFP	Mark Mercola
	21217	PGK-H2BmCherry	Chromatin	H2B	mCherry	Mark Mercola
	20972	H2B-mCherry	Chromatin	H2B	mCherry	Robert Benezra
	80001	pH2B-miRFP703	Chromatin	H2B	miRFP703	Vladislav Verkhusha
	17650	GFP-HP1gamma	Chromatin	HP1 gamma	GFP	Tom Misteli
	55068	mCherry-LaminA-C-18	Nuclear Envelope	LaminA1	mCherry*	Michael Davidson
	98831	mScarlet-I-LaminB	Nuclear Envelope	LaminB	mScarlet-I	Dorus Gadella
	98830	mTurquoise2-LaminB	Nuclear Envelope	LaminB	mTurquoise2	Dorus Gadella
	55069	mCherry-LaminB1-10	Nuclear Envelope	LaminB1	mCherry*	Michael Davidson
	98822	Lck-mTurquoise2	Plasma Membrane	Lck N-terminal lipidation signal	mTurquoise2	Dorus Gadella
	98821	Lck-mScarlet-I	Plasma Membrane	Lck N-terminal lipidation signal	mScarlet-I	Dorus Gadella
	50057	pLYS1-FLAG-MitoGFP-HA	Mitochondria	MCU	GFP	Vamsi Mootha
	49153	GFP-Mff	Mitochondria-Outer Membrane	Mff	acGFP1	Gia Voeltz
	158007	pCMV-mGold-Mito-N-7	Mitochondria	Cox8A	mGold	Francois St-Pierre
	49151	mito-BFP	Mitochondria	mitochondrial targeting signal (COX4)	TagBFP	Gia Voeltz
	55102	mCherry-Mito-7	Mitochondria	Mitochondrial targeting sequence(COX8A)	mCherry*	Michael Davidson
	37354	pQC NLS mCherry IX	Nucleus	NLS	mCherry	Connie Cepko
	37341	pQC NLS YFP IX	Nucleus	NLS	YFP	Connie Cepko
	158000	pCaggs-NLS-PAmCherry1-GSS-EGFP	Nucleus	NLS	mGold	Francois St-Pierre
	14893	pEBFP2-Nuc	Nucleus	NLS	EBFP2	Robert Campbell
	14894	pmKalama1-Nuc	Nucleus	NLS	mKalama1	Robert Campbell
	73205	pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3	Nucleus	NLS (from Mak16p protein)	dL5 FAP; mCerulean3	Marcel Bruchez
	73207	pcDNA3.1-KozATG-dL5-2XG4S-mCer3	Cytosol, nucleoplasm	None	dL5 FAP; mCerulean3	Marcel Bruchez
	36206	pmTurquoise2-NES	Non-nucleus	Nuclear Export Sequence	mTurquoise2	Dorus Gadella
	85062	pmScarlet-i_NES_C1	Non-nucleus	Nuclear Export Sequence	mScarlet-I	Dorus Gadella
	85061	pmScarlet-H_NES_C1	Non-nucleus	Nuclear Export Sequence	mScarlet-H	Dorus Gadella
	85060	pmScarlet_NES_C1	Non-nucleus	Nuclear Export Sequence	mScarlet	Dorus Gadella
	37351	pQC membrane TdTomato IX	Membrane	Palmitoylation	TdTomato	Connie Cepko
	22479	FUmGW	Membrane	Palmitoylation	GFP	Connie Cepko
	14757	pCAG-mGFP	Membrane	Palmitoylation sequence from GAP43	EGFP	Connie Cepko
	61099	Lck-GFP	Membrane	Palmitoylation sequence from Lck	EGFP	Steven Green
	36209	pPalmitoyl-mTurquoise2	Membrane	Palmitoylation sequence from p63	mTurquoise2	Dorus Gadella
	73206	pcDNA3.1-kappa-myc-dL5-2xG4S-TMst	Cell surface (mammalian)	PDGFR-derived	dL5 FAP; mCerulean3	Marcel Bruchez
	45944	pTDpelB-C_sfYFPTwinStrep	Periplasmic space	PelB signal sequence	YFP	Thorben Dammeyer
	54520	mCherry-Peroxisomes-2	Peroxisome	Peroximal Targeting Signal 1 (PTS1)	mCherry*	Michael Davidson
	36203	pmTurquoise2-Peroxi	Peroxisome	Peroxisomal Targeting Sequence	mTurquoise2	Dorus Gadella
	17990	pEGFP-SF2	Nucleus	SF2	EGFP	Tom Misteli
	137806	mNeonGreen-peroxi	Peroxisome	SKL	mNeonGreen	Dorus Gadella
	73210	pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL	Peroxisome	SKL tripeptide, peroxisome transport signal	dL5 FAP; mCerulean3	Marcel Bruchez
	85065	pmScarlet-I_peroxisome_C1	Peroxisome	SRL	mScarlet-I	Dorus Gadella
	85064	pmScarlet-H_peroxisome_C1	Peroxisome	SRL	mScarlet-H	Dorus Gadella
	85063	pmScarlet_peroxisome_C1	Peroxisome	SRL	mScarlet	Dorus Gadella
	55146	mCherry-TOMM20-N-10	Mitochondria-Outer Membrane	TOMM20	mCherry*	Michael Davidson
	118736	pBOB-CARMIL2 BH domain-GFP	Plasma Membrane	CARMIL2 BH domain	GFP	John Cooper
	118737	pBOB-CARMIL2 BH domain-tdTomato	Plasma Membrane	CARMIL2 BH domain	tdTomato	John Cooper

*Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection.

Cytoskeletal Elements

The cytoskeleton is the cell’s internal framework that assists in sustaining structure and function. The cytoskeleton is primarily made up of microtubules, actin filaments, and intermediate filaments. These structures give the cell its shape, organize cellular organelles, and are necessary for movement and cell division. Browse or search the table below to find markers of cytoskeletal element.

	ID	Plasmid	Structure	Targeting Gene/Signal	Fluorescent Protein	PI
	26722	GFP-cortactin	Actin Filaments	Cortactin	EGFP	Anna Huttenlocher
	32907	pEGFP-rNFM	Intermediate Filaments (neuronal)	Nefm	EGFP	Anthony Brown
	32909	pEGFP-mNFM	Intermediate Filaments (neuronal)	Nefm	EGFP	Anthony Brown
	11908	pEGFP-N1 alpha-actinin 1	Actin Filaments	alpha-actinin 1	EGFP	Carol Otey
	27676	Cortactin-pmCherryC1	Actin Filaments	Cortactin	mCherry	Christien Merrifield
	36201	pLifeAct-mTurquoise2	Actin Filaments	LifeAct	mTurquoise2	Dorus Gadella
	36202	pmTurquoise2-Tubulin	Microtubules	alpha-tubulin	mTurquoise2	Dorus Gadella
	85045	pmScarlet_alphaTubulin_C1	Microtubules	alpha-tubulin	mScarlet	Dorus Gadella
	85046	pmScarlet-H_alphaTubulin_C1	Microtubules	alpha-tubulin	mScarlet-H	Dorus Gadella
	85047	pmScarlet-i_alphaTubulin_C1	Microtubules	alpha-tubulin	mScarlet-I	Dorus Gadella
	85054	pLifeAct_mScarlet_N1	Actin Filaments	LifeAct	mScarlet	Dorus Gadella
	85055	pLifeAct_mScarlet-H_N1	Actin Filaments	LifeAct	mScarlet-H	Dorus Gadella
	85056	pLifeAct_mScarlet-i_N1	Actin Filaments	LifeAct	mScarlet-I	Dorus Gadella
	137801	EMTB-mScarletI	Microtubules	EMTB	mScarletI	Dorus Gadella
	137802	EMTB-mNeonGreen	Microtubules	EMTB	mNeonGreen	Dorus Gadella
	137803	EMTB-mTurquoise2	Microtubules	EMTB	mTurquoise2	Dorus Gadella
	137807	mTurquoise2-MapTau	Microtubules	MapTau	mTurquoise2	Dorus Gadella
	137808	mNeonGreen-MapTau	Microtubules	MapTau	mNeonGreen	Dorus Gadella
	137811	ITPKA-mTurquoise2	Actin Filaments	ITPKA	mTurquoise2	Dorus Gadella
	58473	pEGFP-C1 F-tractin-EGFP	Actin Filaments	F-tractin	EGFP	Dyche Mullins
	41147	pEGFP Centrin2	Centrioles (dependent on cell cycle)	Centrin-2	EGFP	Erich Nigg
	41151	pEGFP Cep170 C-term	Centrioles (dependent on cell cycle)	Cep170	EGFP	Erich Nigg
	41163	pEGFP PICH	Centromeres (dependent on cell cycle)	PICH	EGFP	Erich Nigg
	158008	pCMV-mGold-Tubulin-C-18	Microtubules	alpha-tubulin	mGold	Francois St-Pierre
	158009	pCMV-mGold-Actin-C-18	Actin Filaments	Actin	mGold	Francois St-Pierre
	49149	mCh-alpha-tubulin	Microtubules	alpha-tubulin	mCherry	Gia Voeltz
	89472	GFP-hChibby1	Mother Centrioles / Ciliary Base	Chibby1	GFP	Ken-Ichi Takemaru
	89770	pLenti-EGFP-hChibby1	Mother Centrioles / Ciliary Base	Chibby1	EGFP	Ken-Ichi Takemaru
	118084	pLenti-EB1-EGFP	Microtubules	EB1	EGFP	Ken-Ichi Takemaru
	122871	GFP-hCCDC11	Centriolar satellites	CCDC11	EGFP	Ken-Ichi Takemaru
	54234	mEmerald-PLK1-N-16	Centrosome	Plk1	mEmerald*	Michael Davidson
	54491	mCherry-Lifeact-7	Actin Filaments	LifeAct	mCherry*	Michael Davidson
	54610	mEGFP-Lifeact-7	Actin Filaments	LifeAct	EGFP	Michael Davidson
	55148	mCherry-Tubulin-C-18	Microtubules	alpha-tubulin	mCherry*	Michael Davidson
	12298	pIRESneo-EGFP-alpha Tubulin	Microtubules	alpha-tubulin	EGFP	Patricia Wadsworth
	27123	eTC GFP beta-actin full length	Actin Filaments	beta-actin	EGFP	Robert Singer
	27382	pDEST/N1-hEB1-GFP	Microtubules	EB1	GFP	Robin Shaw
	40908	pDEST/LIfeAct-mCherry-N1	Actin Filaments	LifeAct	mCherry	Robin Shaw
	21948	pCAG-mGFP-Actin	Actin Filaments	beta-actin	EGFP	Ryohei Yasuda
	31930	pPAmCherry-a-tubulin	Microtubules	alpha-tubulin	PAmCherry	Vladislav Verkhusha
	31949	pPAmCherry-b-actin	Actin Filaments	beta-actin	PAmCherry	Vladislav Verkhusha
	79990	pKeratin-miRFP703	Intermediate Filaments (epithelial)	Keratin	miRFP703	Vladislav Verkhusha
	79991	pmiRFP703-Tubulin	Microtubules	alpha-tubulin	miRFP703	Vladislav Verkhusha
	79993	pLifeAct-miRFP703	Actin Filaments	LifeAct	miRFP703	Vladislav Verkhusha
	79994	pEB3-miRFP703	Microtubules	EB3	miRFP703	Vladislav Verkhusha
	79996	pVimentin-miRFP703	Intermediate Filaments (vimentin)	Vimentin	miRFP703	Vladislav Verkhusha
	26737	GFP-UtrCH	Actin Filaments	Utrophin (aa# 1-261)	GFP	William Bement
	26740	mCherry-UtrCH	Actin Filaments	Utrophin	mCherry	William Bement
	26741	EMTB-3XGFP	Microtubules	ensconsin	GFP	William Bement
	26742	EMTB-mCherry	Microtubules	ensconsin	mCherry	William Bement

*Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection.

Cell Junctions

Cell junctions are responsible for forming connections and adhesions between animal cells. They are important for holding cell structures together and reducing cell stress. There are four types of cell junctions: adherens junctions, gap junctions, tight junctions, and desmosomes. Browse or search the table below to find markers for cellular junctions.

	ID	Plasmid	Structure	Targeting Gene/Signal	Fluorescent Protein	PI
	71367	Human Beta-catenin GFP	Adherens Junctions	Beta-catenin	EGFP	Alpha Yap
	67937	mouse E-cadherin GFP (423)	Adherens Junctions	E-cadherin	EGFP	Alpha Yap
	71366	Murine E-cadherin mCherry	Adherens Junctions	E-cadherin	mCherry	Alpha Yap
	54219	mEmerald-Paxillin-22	Focal Adhesions	paxillin	mEmerald*	Michael Davidson
	15233	Paxillin-pEGFP	Focal Adhesions	paxillin	GFP	Rick Horwitz
	26720	RFP-zyxin	Focal Adhesions	Zyxin	RFP	Anna Huttenlocher
	11908	pEGFP-N1 alpha-actinin 1	Focal Adhesions	alpha-actinin 1	EGFP	Carol Otey
	31923	pPaxillin-PSmOrange	Focal Adhesions	paxillin	PSmOrange	Vladislav Verkhusha
	79999	pZyxin-miRFP703	Focal Adhesions	Zyxin	miRFP703	Vladislav Verkhusha
	55023	mCherry-Cx43-7	Gap Junctions	Connexin 43 / GJA1-20k	mCherry*	Michael Davidson
	40907	pDEST/hCx43-EGFP-N1	Gap Junctions	Connexin 43	EGFP	Robin Shaw
	49861	pDEST-mCherry-GJA1-20k-N1	Gap Junctions	Connexin 43 / GJA1-20k	mCherry	Robin Shaw
	69007	Cx43-msfGFP	Gap Junctions	Connexin 43 / GJA1	sfGFP	David Spray
	54959	mApple-VE-Cadherin-N-10	Tight Junctions	VE-Cadherin	mApple	Michael Davidson
	55165	mCherry-ZO1-C-14	Tight Junctions	Zonula Occludens-1	mCherry	Michael Davidson
	55001	mCherry-Beta-Catenin-20	Adherens Junctions	Beta-Catenin	mCherry	Michael Davidson
	30313	pEGFP ZO1	Tight Junctions	Zonula Occludens-1	GFP	Alan Fanning

*Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection.

Secretory Pathway

The secretory pathway is named for its role in secreting proteins from the cell to the extracellular environment. Proteins are first translated at the endoplasmic reticulum, then are transported to the Golgi apparatus in vesicles to be packaged and shipped. Many of the proteins that enter the secretory pathway require chaperones to help with folding or post-translational modification to function properly. Browse or search the table below to find markers of secretory pathway components.

	ID	Plasmid	Structure	Targeting Gene/Signal	Fluorescent Protein	PI
	110060	FUW mCherry-GFP-LC3	Autophagosome	LC3	mCherry, GFP	Anne Brunet
	40827	mCherry-hLC3B-pcDNA3.1	Autophagosome	LC3	mCherry	David Rubinsztein
	11546	EGFP-LC3	Autophagosome	LC3	EGFP	Karla Kirkegaard
	61205	pCMB-AUTr	Autophagosome	LC3(ATG8)	mCherry	Maria Harrison
	38277	pMXs-puro GFP-p62	Autophagosome	Sequestosome-1	EGFP	Noboru Mizushima
	38272	pMXs-IP GFP-WIPI-1	Autophagosome	WIPI1	EGFP	Noboru Mizushima
	21074	ptfLC3	Autophagosome	LC3	EGFP	Tamotsu Yoshimori
	21073	pEGFP-LC3	Autophagosome	LC3	EGFP	Tamotsu Yoshimori
	24920	pEGFP-LC3 (human)	Autophagosome	LC3	EGFP	Toren Finkel
	14435	mRFP-Clc	Clathrin-coated vesicles	Clathrin, light polypeptide	mRFP	Ari Helenius
	70217	pEGFP-GFP11-Clathrin light chain	Clathrin-coated vesicles	Clathrin light chain	GFP	Bo Huang
	54040	mEmerald-Clathrin-15	Clathrin-coated vesicles	Clathrin	mEmerald*	Michael Davidson
	79997	pmiRFP703-Clathrin	Clathrin-coated vesicles	Clathrin	miRFP703	Vladislav Verkhusha
	21741	Clathrin-LCa-EYFP	Clathrin-coated vesicles	Clathrin, light polypeptide	EYFP	Xiaowei Zhuang
	14437	mRFP-Rab5	Early endosomes	RAB5A	mRFP	Ari Helenius
	61801	GFP-Rab4B	Early endosomes	Rab4B	AcGFP	Gia Voeltz
	49201	mCh-Rab5	Early endosomes	Rab5	mCherry	Gia Voeltz
	49147	BFP-Rab5	Early endosomes	Rab5	BFP	Gia Voeltz
	61802	GFP-Rab5B	Early endosomes	Rab5B	AcGFP	Gia Voeltz
	79802	pTag-RFP-C-h-Rab5a-c-Myc	Early endosomes	Rab5a	TagRFP	James Johnson
	79801	pTag-BFP-C-h-Rab5a-c-Myc	Early endosomes	Rab5a	TagBFP	James Johnson
	13050	DsRed-Rab5 WT	Early endosomes	RAB5A	DsRed2	Richard Pagano
	42307	GFP-EEA1 wt	Early endosomes	EEA1	GFP	Silvia Corvera
	42635	TagRFP-T-EEA1	Early endosomes	EEA1	TagRFP-T	Silvia Corvera
	38770	pEF.myc.ER-E2-Crimson	Endoplasmic Reticulum	ER retention signal	E2-Crimson	Benjamin Glick
	36204	pmTurquoise2-ER	Endoplasmic Reticulum	ER retention signal	mTurquoise2	Dorus Gadella
	137804	ER-mNeonGreen	Endoplasmic Reticulum	KDEL sequence	mNeonGreen	Dorus Gadella
	137805	ER-mScarletI	Endoplasmic Reticulum	KDEL sequence	mScarlet-I	Dorus Gadella
	68072	ERmoxGFP	Endoplasmic Reticulum	ER retention signal	moxGFP	Erik Snapp
	68126	ERoxBFP	Endoplasmic Reticulum	ER retention signal	oxBFP	Erik Snapp
	49150	BFP-KDEL	Endoplasmic Reticulum	BiP ER signal sequence	BFP	Gia Voeltz
	61807	Rtn4a-GFP	Endoplasmic Reticulum	Rtn4a	AcGFP	Gia Voeltz
	49155	mCh-Sec61 beta	Endoplasmic Reticulum	Sec61	mCherry	Gia Voeltz
	49154	BFP-Sec61 beta	Endoplasmic Reticulum	Sec61	BFP	Gia Voeltz
	73209	pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL	Endoplasmic Reticulum	KDEL	dL5 FAP; mCerulean3	Marcel Bruchez
	73209	pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL	Endoplasmic Reticulum	KDEL	dL5 FAP; mCerulean3	Marcel Bruchez
	85068	pCytERM_mScarlet-i_N1	Endoplasmic Reticulum	CytERM	mScarlet-I	Dorus Gadella
	85067	pCytERM_mScarlet-H_N1	Endoplasmic Reticulum	CytERM	mScarlet-H	Dorus Gadella
	85066	pCytERM_mScarlet_N1	Endoplasmic Reticulum	CytERM	mScarlet	Dorus Gadella
	158004	pCMV-mGold-Calnexin-N-14	Endoplasmic Reticulum	Calnexin	mGold	Francois St-Pierre
	158002	pCMV-mGold-Rab4a-C-7	Endosomes	Rab4a	mGold	Francois St-Pierre
	14873	GolgiCFP	Golgi apparatus	eNOS(1-33)	CFP	Alexandra Newton
	36205	pmTurquoise2-Golgi	Golgi apparatus	B4GALT1(1-61)	mTurquoise2	Dorus Gadella
	68073	GalToxBFP	Golgi apparatus	GalT signal anchor transmembrane domain	oxBFP	Erik Snapp
	158005	pCMV-mGold-MannII-N-10	Golgi apparatus	MannII	mGold	Francois St-Pierre
	85050	pmScarlet-i_Giantin_C1	Golgi apparatus	Giantin	mScarlet-I	Dorus Gadella
	85049	pmScarlet-H_Giantin_C1	Golgi apparatus	Giantin	mScarlet-H	Dorus Gadella
	85048	pmScarlet_Giantin_C1	Golgi apparatus	Giantin	mScarlet	Dorus Gadella
	129630	mTurquoise2-Giantin	Golgi apparatus	Giantin	mTurquoise2	Dorus Gadella
	98880	mNeonGreen-Giantin	Golgi apparatus	Giantin	mNeonGreen	Dorus Gadella
	61804	mCh-Rab7A	Late endosomes	Rab7a	mCherry	Gia Voeltz
	61803	GFP-Rab7A	Late endosomes	Rab7a	AcGFP	Gia Voeltz
	12605	GFP-rab7 WT	Late endosomes	RAB7	GFP	Richard Pagano
	12661	DsRed-rab7 WT	Late endosomes	RAB7	DsRed	Richard Pagano
	158006	pCMV-mGold-Lysosome-N-20	Lysosome	Lamp1	mGold	Francois St-Pierre
	98882	Lamp1-mNeonGreen	Lysosomes	Lamp1	mNeonGreen	Dorus Gadella
	98828	Lamp1-mTurquoise2	Lysosomes	Lamp1	mTurquoise2	Dorus Gadella
	98827	Lamp1-mScarlet-I	Lysosomes	Lamp1	mScarlet-I	Dorus Gadella
	55073	mCherry-Lysosomes-20	Lysosomes	Lamp1	mCherry*	Michael Davidson
	79998	pLAMP1-miRFP703	Lysosomes	Lamp1	miRFP703	Vladislav Verkhusha
	1816	Lamp1-YFP	Lysosomes	Lamp1	YFP	Walther Mothes
	1817	Lamp1-RFP	Lysosomes	Lamp1	RFP	Walther Mothes
	79806	pTag-RFP-C-h-Rab11a-c-Myc	Recycling endosomes	Rab11a	TagRFP	James Johnson
	79805	pTag-BFP-C-h-Rab11a-c-Myc	Recycling endosomes	Rab11a	TagBFP	James Johnson
	79800	pTag-RFP-C-h-Rab4a-c-Myc	Recycling endosomes	Rab4a	TagRFP	James Johnson
	79799	pTag-BFP-C-h-Rab4a-c-Myc	Recycling endosomes	Rab4a	TagBFP	James Johnson
	12674	GFP-rab11 WT	Recycling endosomes	Rab11a	GFP	Richard Pagano
	12679	DsRed-rab11 WT	Recycling endosomes	Rab11a	DsRed	Richard Pagano

*Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection.