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37245 Results

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Plasmids, Strains, and Pooled Libraries 1–20 of 37245

  1. Plasmid
    #11910
    Depositor
    Insert
    PAGFP Aequorea victoria
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    These plasmids are essentiall...
  2. Plasmid
    #118595
    Depositor
    Purpose
    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.
    Insert
    CDK1 Frog
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  3. Plasmid
    #117686
    Depositor
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Insert
    eSpCas9(1.1) Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
  4. Plasmid
    #41791
    Depositor
    Purpose
    Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev
    Insert
    HIV-1 Gag/Pol and RRE sequences
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  5. Plasmid
    #59722
    Depositor
    Purpose
    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
    Insert
    HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG Synthetic
    Use
    TALEN
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  6. Plasmid
    #35094
    Depositor
    Insert
    deletion of zinc fingers and nuclease synthetic
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    use as a transfection balance...
  7. Plasmid
    #37624
    Depositor
    Purpose
    Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids
    Insert
    EBNA-1 ( BKRF1 human herpesvirus 4 )
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  8. Plasmid
    #121513
    Depositor
    Purpose
    expression of AB1-fused-dcas9 plasmids
    Insert
    dCas9
    Use
    Lentiviral
    Tags
    AB1, P2A-mcherry
    Expression
    Mammalian
    Mutation
    None
  9. Plasmid
    #62518
    Depositor
    Purpose
    Expresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.
    Insert
    eGFP Aqueora Victoria
    Use
    AAV
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  10. Plasmid
    #118596
    Depositor
    Purpose
    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.
    Insert
    CDK1 Frog
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  11. Plasmid
    #118349
    Depositor
    Purpose
    This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.
    Insert
    scrambled shRNA Human
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    WT
  12. Plasmid
    #124808
    Depositor
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.
    Insert
    hSPCas9 Synthetic
    Use
    Tags
    3XFLAG, GFP
    Expression
    Mammalian
    Mutation
    None
  13. Plasmid
    #59723
    Depositor
    Purpose
    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
    Insert
    NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI Synthetic
    Use
    TALEN
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  14. Plasmid
    #124811
    Depositor
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Insert
    Cas9 – gRNA_R2A_hinge Synthetic
    Use
    CRISPR
    Tags
    3XFLAG, GFP
    Expression
    Mammalian , Bacterial
    Mutation
    R166H in PuroR (please see de...
  15. Plasmid
    #11909
    Depositor
    Insert
    pPAGFP-N1 Aequorea victoria
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    These plasmids are essentiall...
  16. Plasmid
    #12259
    Depositor
    Purpose
    VSV-G envelope expressing plasmid
    Insert
    VSV G
    Use
    Lentiviral ; Envelope
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  17. Plasmid
    #65974
    Depositor
    Purpose
    Human codon-optimized T7 polymerase
    Insert
    T7-opt bacteriophage
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  18. Plasmid
    #70219
    Depositor
    Purpose
    Expresses GFP(1-10) in mammalian cells
    Insert
    GFP(1-10) Synthetic
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  19. Plasmid
    #27082
    Depositor
    Purpose
    Integration-free (episomal) expression of EGFP
    Insert
    eGFP
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
  20. Plasmid
    #35617
    Depositor
    Insert
    Ecotropic MLV envelope
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
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