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379 Results

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Plasmids, Strains, and Pooled Libraries 1–20 of 379

  1. Plasmid
    #125808
    Depositor
    Purpose
    Level0 xCas9 3.7
    Insert
    xCas9 3.7 Synthetic
    Use
    CRISPR, Synthetic Biology
    Tags
    None
    Expression
    Plant
    Mutation
    None
  2. Plasmid
    #125813
    Depositor
    Purpose
    Level0 Csy4
    Insert
    Csy4 Synthetic
    Use
    CRISPR, Synthetic Biology
    Tags
    None
    Expression
    Plant
    Mutation
    None
  3. Plasmid
    #50590
    Depositor
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Insert
    T1, U626t plus, U629p, T2 Synthetic
    Use
    CRISPR ; PCR template
    Tags
    None
    Expression
    Plant
    Mutation
    None
  4. pRGEB31 Empty backbone
    Plasmid
    #51295
    Depositor
    Purpose
    Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plant. by agrobacterium mediated transformation.
    Use
    CRISPR ; plant expression
    Tags
    None
    Expression
    Mutation
    None
  5. Plasmid
    #91140
    Depositor
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
  6. Plasmid
    #91145
    Depositor
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
  7. Plasmid
    #86196
    Depositor
    Purpose
    AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing
    Insert
    AsCpf1 gRNA cloning site for ribozyme cleavage Acidaminococcus sp. BV3L6
    Use
    CRISPR
    Tags
    Hammerhead ribozyme and HDV r...
    Expression
    Plant
    Mutation
    None
  8. Plasmid
    #53063
    Depositor
    Purpose
    Transcription of guide RNA (gRNA) in rice cells
    Insert
    OsU3 promoter + gRNA scaffold rice U3 promoter
    Use
    CRISPR ; Plant expression
    Tags
    None
    Expression
    Mutation
    None
  9. Plasmid
    #109328
    Depositor
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter
    Insert
    zCas9
    Use
    CRISPR ; Gateway compatible z...
    Tags
    NLS, 3x FLAG, NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
  10. Plasmid
    #115488
    Depositor
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)
    Insert
    NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR, Synthetic Biology
    Tags
    None
    Expression
    Plant
    Mutation
    None
  11. Plasmid
    #117504
    Depositor
    Purpose
    YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse
    Insert
    BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
  12. Plasmid
    #115628
    Depositor
    Purpose
    Targeted A to G in wheat plants or monocotyledons protoplasts
    Insert
    wtTadA-TadA7.10-nCas9-3*NLS
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
  13. pBAtC Empty backbone
    Plasmid
    #78097
    Depositor
    Purpose
    Plant expression of Cas9 and gRNA empty backbone; Basta resistance
    Use
    CRISPR
    Tags
    NLS-HA
    Expression
    Plant
    Mutation
    None
  14. Plasmid
    #106331
    Depositor
    Purpose
    Expression of Cas9 in plants, ZmUbi promoter, Kanamycin selection
    Insert
    Cas9 Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    plant-codon optimized with hi...
  15. Plasmid
    #115483
    Depositor
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR, Synthetic Biology
    Tags
    None
    Expression
    Plant
    Mutation
    None
  16. Plasmid
    #106437
    Depositor
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter
    Insert
    CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS Human, Mustard Weed, Synthetic
    Use
    CRISPR, Synthetic Biology
    Tags
    None
    Expression
    Plant
    Mutation
    None
  17. Plasmid
    #61477
    Depositor
    Purpose
    based on pDe-CAS9, encodes Cas9 D10A nickase variant
    Insert
    Cas9-D10A Mustard Weed
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    catalytic Asp10 changed to Ala
  18. Plasmid
    #115623
    Depositor
    Purpose
    Targeted A to G in wheat plants or monocotyledons protoplasts
    Insert
    wtTadA-TadA7.10-nCas9-NLS
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
  19. Plasmid
    #115490
    Depositor
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR, Synthetic Biology
    Tags
    None
    Expression
    Plant
    Mutation
    None
  20. pYPQ133A2.0 Empty backbone
    Plasmid
    #99891
    Depositor
    Purpose
    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
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