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295 Results

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Plasmids, Strains, and Pooled Libraries 1–20 of 295

  1. pRGEB31 Empty backbone
    Plasmid
    #51295
    Depositor
    Purpose
    Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plant. by agrobacterium mediated transformation.
    Use
    CRISPR ; plant expression
    Tags
    None
    Expression
    Mutation
    None
  2. Plasmid
    #50590
    Depositor
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Insert
    T1, U626t plus, U629p, T2 Synthetic
    Use
    CRISPR ; PCR template
    Tags
    None
    Expression
    Plant
    Mutation
    None
  3. Plasmid
    #91140
    Depositor
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
  4. Plasmid
    #86196
    Depositor
    Purpose
    AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing
    Insert
    AsCpf1 gRNA cloning site for ribozyme cleavage Acidaminococcus sp. BV3L6
    Use
    CRISPR
    Tags
    Hammerhead ribozyme and HDV r...
    Expression
    Plant
    Mutation
    None
  5. pYPQ133A2.0 Empty backbone
    Plasmid
    #99891
    Depositor
    Purpose
    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
  6. pYPQ131A2.0 Empty backbone
    Plasmid
    #99884
    Depositor
    Purpose
    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
  7. Plasmid
    #91145
    Depositor
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
  8. Plasmid
    #91133
    Depositor
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II
    Insert
    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
  9. Plasmid
    #61477
    Depositor
    Purpose
    based on pDe-CAS9, encodes Cas9 D10A nickase variant
    Insert
    Cas9-D10A Mustard Weed
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    catalytic Asp10 changed to Ala
  10. Plasmid
    #109328
    Depositor
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter
    Insert
    zCas9
    Use
    CRISPR ; Gateway compatible z...
    Tags
    NLS, 3x FLAG, NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
  11. Plasmid
    #91138
    Depositor
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar
    Insert
    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
  12. Plasmid
    #115628
    Depositor
    Purpose
    Targeted A to G in wheat plants or monocotyledons protoplasts
    Insert
    wtTadA-TadA7.10-nCas9-3*NLS
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
  13. Plasmid
    #79444
    Depositor
    Purpose
    recipient plasmid [single nuclease]; p35S:Cas9, nptII
    Insert
    ccdB ( ccdB(letD) )
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    BsaI site eliminated
  14. pBAtC Empty backbone
    Plasmid
    #78097
    Depositor
    Purpose
    Plant expression of Cas9 and gRNA empty backbone; Basta resistance
    Use
    CRISPR
    Tags
    NLS-HA
    Expression
    Plant
    Mutation
    None
  15. Plasmid
    #106331
    Depositor
    Purpose
    Expression of Cas9 in plants, ZmUbi promoter, Kanamycin selection
    Insert
    Cas9 Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    plant-codon optimized with hi...
  16. Plasmid
    #115488
    Depositor
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)
    Insert
    NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR, Synthetic Biology
    Tags
    None
    Expression
    Plant
    Mutation
    None
  17. Plasmid
    #115344
    Depositor
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter
    Insert
    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS Human, Mustard Weed, Synthetic
    Use
    CRISPR, Synthetic Biology
    Tags
    None
    Expression
    Plant
    Mutation
    None
  18. Plasmid
    #75404
    Depositor
    Purpose
    Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional Activator
    Insert
    dCas:EDLL Synthetic
    Use
    CRISPR, Synthetic Biology
    Tags
    None
    Expression
    Plant
    Mutation
    None
  19. Plasmid
    #53063
    Depositor
    Purpose
    Transcription of guide RNA (gRNA) in rice cells
    Insert
    OsU3 promoter + gRNA scaffold rice U3 promoter
    Use
    CRISPR ; Plant expression
    Tags
    None
    Expression
    Mutation
    None
  20. Plasmid
    #117504
    Depositor
    Purpose
    YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse
    Insert
    BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI Synthetic
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    None
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