This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Full Site Search

Searches all Addgene web content.

Search for Plasmids

Optimized to search plasmid data.

Search Results 1–12 of 12

Plasmids

  1. Strain SIJ_488 (Bacterial strain #68246)
    Purpose E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.
    Insert: arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Alex Nielsen
  2. BUN21/pML300 (recipient strain) (Bacterial strain #11675)
    Insert: Red-alpha, Red-beta, Gam Bacteriophage lambda
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Stephen Elledge
  3. EcNGRfC (Bacterial strain #48936)
    Purpose Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)
    Insert: galK::fadBp-CAT, mutS::bla
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: George Church
  4. EcNGRfEfC-fadD-tesA'-fadR (Bacterial strain #48939)
    Purpose Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)
    Insert: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: George Church
  5. EcNGRfEfC-fadD-tesA' (Bacterial strain #48938)
    Purpose Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)
    Insert: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: George Church
  6. EcNGRfEfC (Bacterial strain #48937)
    Purpose Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)
    Insert: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: George Church
  7. EcNGRfE (Bacterial strain #48935)
    Purpose Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)
    Insert: mutS::bla, fadE265G>T/268delG
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: George Church
  8. HB101 endA::frt lambda pir (Bacterial strain #45472)
    Insert: endA::frt lambda pir
    Tags: None
    Expression: Bacterial
    Mutation: major endonuclease endA deleted
    Use:
    Deposited by: Michele Swanson
  9. C321.ΔA.exp (Bacterial strain #49018)
    Purpose This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C
    Insert: Recoded E. coli with all UAG codons and RF1 removed (UAG termination abolished); decreased mutation rate; 37C growth tolerated E. coli
    Tags: None
    Expression: Bacterial
    Mutation: See genbank file
    Use:
    Deposited by: George Church
  10. E. coli K-12 MG1655 RARE (Bacterial strain #61440)
    Purpose This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Kristala Prather
  11. Nuc5-.dnaG.Q576A.tolC- (Bacterial strain #41699)
    Insert: E. coli MG1655 ΔmutS::cat Δ(ybhB-bioAB)::[λcI857 N(cro-ea59)::tetR-bla] xonA-, recJ-,xseA-, exoX- and redα- dnaG.Q576A tolC-
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: George Church
  12. MG1655-tMMR (Bacterial strain #62392)
    Purpose E. coli MG1655 temperature-sensitive mismatch repair deficient strain, harboring mutS(A134V) mutL(G62S), and nadA::Tn10 pglΔ8 [λ cI857 Δ(cro-bioA)]} for λ Red expression
    Insert: MG1655-tMMR E. coli
    Tags: None
    Expression:
    Mutation: mutS(A134V) mutL(G62S); nadA...
    Use:
    Deposited by: Csaba Pál
Sort
Filters clear all

Expression

clear

Plasmid Type

clear

Insert Species

clear

Selectable Marker

clear

Resistance

clear

Popularity

clear

First « Previous
Next » Last