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161 Results for:  bacillus

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Addgene Guides & Resources

Plasmids, Strains, and Pooled Libraries 1–20 of 159

  1. Plasmid
    #107581
    Depositor
    Purpose
    B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Insert
    mCherry Synthetic
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  2. Plasmid
    #107582
    Depositor
    Purpose
    B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Insert
    GFP+ Synthetic
    Use
    Synthetic Biology ; E. coli a...
    Tags
    B. megaterium DSM319 xylA lea...
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T...
  3. Plasmid
    #117122
    Depositor
    Purpose
    Kanamycin resistance cassette
    Insert
    Kanamycin resistance cassette for Bacillus subtilis selection Bacillus subtilis
    Use
    Golden gate donor vector for ...
    Tags
    None
    Expression
    Mutation
    None
  4. Plasmid
    #107578
    Depositor
    Purpose
    B. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Insert
    GFP+ Synthetic
    Use
    Synthetic Biology
    Tags
    B. megaterium DSM319 xylA lea...
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T...
  5. Plasmid
    #117120
    Depositor
    Purpose
    Spectinomycin resistance cassette
    Insert
    Spectinomycin resistance cassette for Bacillus subtilis selection Bacillus subtilis
    Use
    Golden gate donor vector for ...
    Tags
    None
    Expression
    Mutation
    None
  6. Plasmid
    #117121
    Depositor
    Purpose
    Erythromycin resistance cassette
    Insert
    Erythromycin resistance cassette for Bacillus subtilis selection Bacillus subtilis
    Use
    Golden gate donor vector for ...
    Tags
    None
    Expression
    Mutation
    None
  7. Plasmid
    #117119
    Depositor
    Purpose
    Chloramphenicol resistance cassette
    Insert
    Chloramphenicol resistance cassette for Bacillus subtilis selection Bacillus subtilis
    Use
    Golden gate donor vector for ...
    Tags
    None
    Expression
    Mutation
    None
  8. Plasmid
    #107577
    Depositor
    Purpose
    B. megaterium DSM319 gap promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Insert
    GFP+ Synthetic
    Use
    Synthetic Biology ; E. coli a...
    Tags
    B. megaterium DSM319 xylA lea...
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T...
  9. Plasmid
    #107576
    Depositor
    Purpose
    B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Insert
    GFP+ Synthetic
    Use
    Synthetic Biology
    Tags
    B. megaterium DSM319 xylA lea...
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T...
  10. Plasmid
    #48122
    Depositor
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter
    Insert
    predicted optimal signal peptide for Bacillus megaterium protein secretion
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  11. Plasmid
    #73839
    Depositor
    Purpose
    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit Bacillus subtilis
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  12. Plasmid
    #73264
    Depositor
    Purpose
    Produces Bacillus subtilis phosphoribosylformylglycinamidine synthase small subunit (BsPurS)
    Insert
    phosphoribosylformylglycinamidine synthase subunit PurS Bacillus subtilis
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  13. Plasmid
    #117130
    Depositor
    Purpose
    FtsY gene cassette
    Insert
    ftsY Bacillus subtilis
    Use
    Golden gate donor vector for ...
    Tags
    None
    Expression
    Mutation
    None
  14. Plasmid
    #84863
    Depositor
    Purpose
    bacterial expression of Bacillus anthracis protective antigen
    Insert
    PA Bacillus anthracis
    Use
    Tags
    pelB signal sequence
    Expression
    Bacterial
    Mutation
    None
  15. Plasmid
    #84870
    Depositor
    Purpose
    Baculovirus Expression of Bacillus anthracis protective antigen
    Insert
    PA Bacillus anthracis
    Use
    Baculovirus
    Tags
    His
    Expression
    Insect
    Mutation
    None
  16. Plasmid
    #86554
    Depositor
    Purpose
    Expresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficile
    Insert
    Pveg-sLucopt Synthetic; Bacillus subtilis
    Use
    Luciferase, Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  17. Plasmid
    #122851
    Depositor
    Purpose
    Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.
    Insert
    Sfp Bacillus subtilis
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  18. Plasmid
    #103111
    Depositor
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Insert
    G9QLF2(Cas9 coding gene from Bacillus smithii 7_3_47FAA)
    Use
    CRISPR ; Cloning vector
    Tags
    None
    Expression
    Mutation
    human codon-optimized
  19. Plasmid
    #73840
    Depositor
    Purpose
    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit Bacillus subtilis
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    A128T mutant
  20. Plasmid
    #117131
    Depositor
    Purpose
    smc downstream homology region
    Insert
    sivC Bacillus subtilis
    Use
    Golden gate donor vector for ...
    Tags
    None
    Expression
    Mutation
    None
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