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18 Results for:  geobacillus

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Plasmids, Strains, and Pooled Libraries 1–18 of 18

  1. Plasmid
    #100981
    Depositor
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Insert
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain Geobacillus thermodenitrificans T12
    Insert
    ThermoCas9 single guide RNA expressing module Geobacillus thermodenitrificans T12
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  2. Plasmid
    #100982
    Depositor
    Purpose
    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module
    Insert
    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain Geobacillus thermodenitrificans T12
    Insert
    ThermoCas9 single guide RNA expressing module Geobacillus thermodenitrificans T12
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    changed aspartate 8 to alanin...
  3. Plasmid
    #44009
    Depositor
    Insert
    surT-PsurP-agaN Geobacillus stearothermophilus NUB3621
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Deleted EcoRI site (synonymou...
  4. Plasmid
    #87703
    Depositor
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS
    Insert
    GeoCas9 Geobacillus stearothermophilus
    Use
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Mutation
    None
  5. Plasmid
    #71738
    Depositor
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Insert
    PheB Geobacillus stearothermophilus
    Use
    Synthetic Biology ; Shuttle V...
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  6. Plasmid
    #71548
    Depositor
    Purpose
    Produces Geobacillus stearothermophilus (Bacillus stearothermophilus) alanine racemase (alr)
    Insert
    Alanine racemase Geobacillus stearothermophilus
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  7. Plasmid
    #87700
    Depositor
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP
    Insert
    GeoCas9 Geobacillus stearothermophilus
    Use
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Mutation
    None
  8. pG2K Empty backbone
    Plasmid
    #71742
    Depositor
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Use
    Synthetic Biology ; Shuttle V...
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  9. Plasmid
    #71737
    Depositor
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Insert
    mCherry
    Use
    Synthetic Biology ; Shuttle V...
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  10. Plasmid
    #58711
    Depositor
    Purpose
    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag
    Insert
    Xyl-DocI Synthetic; Geobacillus stearothermophilus, Clostridium thermocellum
    Use
    Tags
    HIS, HRV 3C, ybbR
    Expression
    Bacterial
    Mutation
    changed Threonin 120 to Cyste...
  11. Plasmid
    #52217
    Depositor
    Purpose
    Constitutive sfGFP expression plasmid for use in Geobacillus and E. coli
    Insert
    PRHIII-sfGFP Synthetic
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  12. pG1AK Empty backbone
    Plasmid
    #71736
    Depositor
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Use
    Synthetic Biology ; Shuttle V...
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  13. pExp-Lipo Empty backbone
    Plasmid
    #129237
    Depositor
    Purpose
    Protein expression in E.coli
    Use
    Tags
    His, Strep
    Expression
    Bacterial
    Mutation
    None
  14. Plasmid
    #108952
    Depositor
    Purpose
    heterologous expression of FPPS of G. stearothermophilus in E. coli
    Insert
    Farnesyldiphosphat synthase (FPPS) Geobacillus stearothermophilus
    Use
    Tags
    His6-Tag
    Expression
    Bacterial
    Mutation
    None
  15. pG1C Empty backbone
    Plasmid
    #71740
    Depositor
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Use
    Synthetic Biology ; Shuttle V...
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  16. pG1K Empty backbone
    Plasmid
    #71741
    Depositor
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Use
    Synthetic Biology ; Shuttle V...
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  17. Plasmid
    #60578
    Depositor
    Purpose
    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
    Insert
    Integrase_7 Geobacillus sp. Y412MC61
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli
  18. Plasmid
    #71739
    Depositor
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Insert
    sfGFP
    Use
    Synthetic Biology ; Shuttle V...
    Tags
    None
    Expression
    Bacterial
    Mutation
    None