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54 Results for:  toxoplasm*

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Plasmids, Strains, and Pooled Libraries 1–20 of 54

  1. Pooled library
    #80636
    Depositor
    Purpose
    Plasmid sgRNA library containing ten guides against each of the 8,158 predicted Toxoplasma gondii protein coding genes.
    Insert
    ~80,000 sgRNAs, ten each against each of the 8,158 predicted Toxoplasma gondii protein coding genes
    Use
    Tags
    None
    Expression
    Mutation
    None
  2. Plasmid
    #52694
    Depositor
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Insert
    Cas9 Synthetic; Originally from Streptococcus progenies, codon-optimized for Plasmodium falciparum
    Insert
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA Synthetic; Toxoplasma gondii, streptococcus pyogenes
    Use
    CRISPR ; Toxoplasma gondii
    Tags
    Nuclear localization signal, ...
    Expression
    Mutation
    None
  3. Plasmid
    #86552
    Depositor
    Purpose
    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma
    Insert
    Linker-6xHA-HGPRT-LoxP Toxoplasma gondii
    Use
    Expression in Toxoplasma gondii
    Tags
    None
    Expression
    Mutation
    None
  4. Plasmid
    #78706
    Depositor
    Purpose
    GCaMP5 under the Toxoplasma SAG1 5' UTR. Encodes a DHFR selectable marker.
    Insert
    GCaMP5 Synthetic
    Insert
    DHFR Toxoplasma gondii
    Use
    Tags
    None
    Expression
    Mutation
    None
  5. Plasmid
    #86553
    Depositor
    Purpose
    used for a TIR1-AID system with CRISPR tagging technology in Toxoplasma gondii
    Insert
    Linker-AID-3xHA-HXGPRT 3'UTR Toxoplasma gondii
    Use
    Expression in Toxoplasma gondii
    Tags
    None
    Expression
    Mutation
    None
  6. Plasmid
    #78705
    Depositor
    Purpose
    GCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable marker
    Insert
    GCaMP5 Synthetic
    Use
    Tags
    None
    Expression
    Mutation
    None
  7. Plasmid
    #54467
    Depositor
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.
    Insert
    SAG1 5' UTR Toxoplasma gondii
    Use
    CRISPR ; Toxoplasma expression
    Tags
    None
    Expression
    Mutation
    None
  8. pLIC-SM-HA Empty backbone
    Plasmid
    #111188
    Depositor
    Purpose
    High affinity HA Tagging of weakly expressed genes (optimized for Toxoplasma gondii)
    Use
    Toxoplasma gondii expression
    Tags
    Spaghetti Monster HA Tag
    Expression
    Mutation
    None
  9. Plasmid
    #100605
    Depositor
    Purpose
    pUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTR
    Insert
    Floxed TgDHFR-TS* Toxoplasma gondii
    Use
    Toxoplasma expression
    Tags
    None
    Expression
    Mutation
    Pyrimethamine resistance
  10. Plasmid
    #59018
    Depositor
    Purpose
    Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii
    Insert
    YFP-TetR Toxoplasma gondii
    Use
    Toxoplasma Expression
    Tags
    None
    Expression
    Mutation
    None
  11. Plasmid
    #86667
    Depositor
    Purpose
    for generation of AID strains using CRISPR tagging technology
    Insert
    Linker-AID-Ty-HGPRT 3'UTR Toxoplasma gondii
    Use
    Expression in Toxoplasma gondii
    Tags
    None
    Expression
    Mutation
    None
  12. Plasmid
    #86669
    Depositor
    Purpose
    for generation of AID strains using CRISPR tagging technology
    Insert
    Linker-AID-3xHA-HXGPRT 3'UTR Toxoplasma gondii
    Use
    Expression in Toxoplasma gondii
    Tags
    None
    Expression
    Mutation
    None
  13. Plasmid
    #86664
    Depositor
    Purpose
    CRISPR tagging with 2xTy in Toxoplamsa gondii
    Insert
    Linker-2xTy-HXGPRT 3'UTR Toxoplasma gondii
    Use
    Expression in Toxoplasma gondii
    Tags
    None
    Expression
    Mutation
    None
  14. Plasmid
    #100606
    Depositor
    Purpose
    TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.
    Insert
    TgUPRT::Floxed TgDHFR-TS* Toxoplasma gondii
    Use
    Toxoplasma expression
    Tags
    None
    Expression
    Mutation
    Pyrimethamine resistance
  15. Plasmid
    #50051
    Depositor
    Purpose
    expresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells
    Insert
    M2AP toxoplasma gondii
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    starts at aa47; N127R, S158A,...
  16. pLIC-SM-Myc Empty backbone
    Plasmid
    #111189
    Depositor
    Purpose
    High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)
    Use
    Toxoplasma gondii expression
    Tags
    Spaghetti Monster Myc Tag
    Expression
    Mutation
    None
  17. Plasmid
    #80329
    Depositor
    Purpose
    Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine
    Insert
    DHFR-TSc3 Toxoplasma gondii
    Use
    CRISPR
    Tags
    None
    Expression
    Mutation
    None
  18. Plasmid
    #70147
    Depositor
    Purpose
    Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion
    Insert
    a selectable dihydrofolate reductase-thymidylate synthase marker Toxoplasma gondii
    Use
    Expression in Toxoplasma gondii
    Tags
    mCherry
    Expression
    Mutation
    None
  19. Plasmid
    #59855
    Depositor
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.
    Insert
    CRISPR sg290860-6
    Use
    CRISPR ; ; Toxoplasma gondii
    Tags
    None
    Expression
    Mutation
    None
  20. Plasmid
    #50050
    Depositor
    Purpose
    expresses toxoplasma gondii MIC2 extracellular domain in mammalian cells
    Insert
    MIC2 toxoplasma gondii
    Use
    Tags
    SBP, HIS, TEV
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
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