We narrowed to 2,014 results for: cp

1. pCA528 IST1 L355A (316-366)(C-Cys)

   Plasmid

   #193036

   Purpose

   Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L355A mutation that diminishes binding to MIT domains.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   L355A, Residues 316-366, Non-native C-terminal Cy…

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCA528 IST1 L328D,L355A (316-366)(C-Cys)

   Plasmid

   #193037

   Purpose

   Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D and L355A mutations that diminishes binding to MIT domains.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   L328D, L355A, Residues 316-366, Non-native C-term…

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MT4

   Plasmid

   #223332

   Purpose

   Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

   Depositor

   Xiaohong Zhu

   Article

   Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

   Insert

   3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)
   

   Tags

   3HA-miniTurboID-EGFP

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. HNRNPA1-miRFP670-TRF1

   Plasmid

   #227384

   Purpose

   Adhesion module, creates 'seed' at telomere

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   Telomeric HNRNPA1 IDR (TERF1, HNRNPA1 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAT079

   Plasmid

   #180509

   Purpose

   Plasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   DpbCasX
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-f1(+)-SEC61B-dTomato

   Plasmid

   #115913

   Purpose

   donor to insert dTomato at the SEC61B locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   SEC61B-dTomato (SEC61B Human)
   

   Use

   Donor template

   Tags

   dTomato

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-f1(+)-TOMM20-dTomato

   Plasmid

   #115914

   Purpose

   donor to insert dTomato at the TOMM20 locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   TOMM20-dTomato (TOMM20 Human)
   

   Use

   Donor template

   Tags

   dTomato

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

   Plasmid

   #91819

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Tags

   10xHis

   Expression

   Yeast

   Mutation

   5xGly linker, PreScission Protease site (LEVLFQGP…

   Promoter

   Endogenous Rpb1 promoter

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

   Plasmid

   #91820

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Tags

   10xHis

   Expression

   Yeast

   Mutation

   5xGly linker, PreScission Protease site (LEVLFQGP…

   Promoter

   Endogenous Rpb1 promoter

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMY-CD90.2-P2A-BlastDYK-Rluc_miR

    Plasmid

    #163341

    Purpose

    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and a DYK-tagged Blasticidin selection marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD90.2 (Thy1 Mouse)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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