We narrowed to 4,342 results for: Pet2;

1. pET20b-GpNLuc-6xHis

   Plasmid

   #135932

   Purpose

   Expresses the GpNLuc LumiFluor in bacterial cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   GpNLuc LumiFluor
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_6xHis-PARP1delWGR-L713F

   Plasmid

   #173941

   Purpose

   Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delWGR-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 521-660; L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-LaG-27

   Plasmid

   #172752

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-27
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a_6HT-TBP6.7(R49A)

   Plasmid

   #112726

   Purpose

   Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R49A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R49A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. eGFP-Silicatein-pET28

   Plasmid

   #198012

   Purpose

   C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification

   Depositor

   Bryan Berger

   Article

   Vigil et al Materials Advances 2, 2023

   Insert

   eGFP-silicatein
   

   Tags

   hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET20b-delta133HtrA2 A141S

   Plasmid

   #16157

   Depositor

   L. Miguel Martins

   Article

   Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

   Insert

   HtrA2 (HTRA2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   A141S

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28 Set9 mutant

   Plasmid

   #24083

   Depositor

   Danny Reinberg

   Article

   Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

   Insert

   Set9 (SETD7 Human)
   

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   H297A

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

8. TrmD_HaeIn/pET28b(+) N,C-tag

   Plasmid

   #12665

   Depositor

   SeWon Suh

   Article

   Kim et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21a-mAIM2-deltaPYD

   Plasmid

   #51535

   Purpose

   Express His6-tagged HIN domain of mAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   Aim2 (Aim2 Mouse)
   

   Tags

   His6 and T7

   Expression

   Bacterial

   Promoter

   lac operator

   Available Since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET24a-LaM4-1xTS

    Plasmid

    #162778

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, T7, and TS site from proCCK

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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