We narrowed to 615 results for: vp64

1. LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

   Plasmid

   #47457

   Purpose

   LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   NLS(alpha-imp)-CRY2PHR-NLS-VP64
   

   Tags

   2A_GFP

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)

   Plasmid

   #194222

   Purpose

   Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   SynNotch-GAL4 DBD-VP64 AD
   

   Use

   Synthetic Biology

   Promoter

   None

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101102

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   TMt-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA and Myc

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

   Plasmid

   #47455

   Purpose

   LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   NLS(alpha-imp)-CRY2PHR-NLS-VP64
   

   Use

   AAV

   Tags

   2A_GFP

   Expression

   Mammalian

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available Since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101107

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   BDKRB2-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101115

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to LPAR1

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   LPAR1-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101113

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to CXCR4

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   CXCR4-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD1122 CMV 3xFLAG-NES-PYL1-VP64 in TUPV3

   Plasmid

   #161547

   Purpose

   Constitutive expression of 3xFLAG-NES-PYL1-VP64 under the CMV promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NES-PYL1-VP64
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101111

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to AVPR2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   AVPR2-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJM582 EF1α 3xFLAG-NLS-VP64-ZF2 in TUPV2

    Plasmid

    #161543

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-ZF2 under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-ZF2
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJM465 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2

    Plasmid

    #161533

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-ZF1
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64

    Plasmid

    #101110

    Purpose

    Encodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(C)_DHFR-PCP-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. TetO-FUW-FLAG-NLS-VP64-MCS_N-term

    Plasmid

    #136886

    Purpose

    Doxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    VP64 activator domain
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJM554 EF1α 3xFLAG-NLS-VP64-intN in TUPV2

    Plasmid

    #161556

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-intN under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-intN
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJM571 ZF2x6-C 3xFLAG-NLS-VP64-intN in TUPV2

    Plasmid

    #161558

    Purpose

    Inducible expression of 3xFLAG-NLS-VP64-intN under ZF2x6-C the promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-intN
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Promoter

    ZF2x6-C

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJM583 EF1α 3xFLAG-NLS-VP64-ZF6 in TUPV2

    Plasmid

    #161544

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-ZF6 under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-ZF6
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJM512 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2

    Plasmid

    #161538

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-ZF1
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJM520 EF1α 3xFLAG-NLS-VP64-ZF10 in TUPV4

    Plasmid

    #161539

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-ZF10 under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-ZF10
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. dSp-VP64-p65(100-261)-RTA(75-190)

    Plasmid

    #99672

    Purpose

    Expresses dSp Cas9 fused to combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    VP64-p65(101-261)-RTA(75-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid

    #183409

    Purpose

    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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