We narrowed to 4,418 results for: 1-Oct;

1. pDGO186N-KS1

   Plasmid

   #174301

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Expression

   Bacterial

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEW100cre-EP1

   Plasmid

   #33345

   Purpose

   For expression of tetracycline-inducible CRE in Trypanosoma brucei

   Depositor

   George Cross

   Article

   Scahill et al Mol Biochem Parasitol. 2008 Jan;157(1):7

   Insert

   cre recombinase
   

   Use

   Cre/Lox; Trypanosoma brucei expression vector

   Promoter

   TET operator

   Available Since

   Dec. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBA2kIKmA2

   Plasmid

   #39213

   Depositor

   Anastasios Melis

   Article

   Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

   Insert

   Kudzu Isoprene Synthase
   

   Use

   Synthetic Biology

   Mutation

   Deleted the predicted chloroplast transit peptide

   Promoter

   psbA2

   Available Since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pYS1

   Plasmid

   #117488

   Purpose

   Generation of unmarked gene deletions in Mycobacterium smegmatis

   Depositor

   Eyal Gur

   Article

   Shenkerman et al Gene. 2014 Jan 1;533(1):374-8. doi: 10.1

   Insert

   Che9c 60–61, sacB, aph
   

   Use

   Deletion generation

   Promoter

   Acetamide

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDH008

   Plasmid

   #157643

   Purpose

   sMHT-113 fused to CheY only

   Depositor

   Jonathan Silberg

   Article

   Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

   Inserts

   sMHT(1-113)
   CheY-sMHT(1-114)
   

   Expression

   Bacterial

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR221-JUN

   Plasmid

   #79512

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   JUN (JUN Human)
   

   Use

   Multisite gateway entry clone

   Tags

   HA Tag

   Promoter

   no Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO-TRE-AFOS

   Plasmid

   #197079

   Purpose

   A doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.

   Depositor

   Elaine Fuchs

   Article

   Larsen et al Cell Stem Cell. 2021 Oct 7;28(10):1758-1

   Insert

   AFOS (Fos Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and T7

   Promoter

   TRE

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. LCV2 V-3

   Plasmid

   #84806

   Purpose

   Cas9 with sgRNA targeting Exon 1 of VEGF-A

   Depositor

   Glenn Yiu

   Article

   Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

   Insert

   gRNA targeting VEGF-A (VEGFA Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4/HisMaxB-YAP1-S127A

   Plasmid

   #18988

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Yes-kinase associated protein S127A mutant (YAP1 Human)
   

   Tags

   His

   Expression

   Mammalian

   Mutation

   Serine 127 changed to Alanine, mutated protein t…

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

10. RBD-Fc

    Plasmid

    #164221

    Purpose

    Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    Spike RBD (S SARS-CoV)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac Dual α1B/β2B Tubulin

    Plasmid

    #170553

    Purpose

    Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB2B (TUBB2B Human)
    

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac Dual α1B/β3 Tubulin

    Plasmid

    #170552

    Purpose

    Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB3 (TUBB3 Human)
    

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. SP-GFP-CD274-NU

    Plasmid

    #121139

    Purpose

    Mammalian expression of GFP tagged CD274

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Inserts

    CD274 (CD274 Human)
    CD274 (CD274 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2xFlag CMV2-YAP2-1st WW mutant

    Plasmid

    #19046

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein isoform 3 (YAP1 Human)
    

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Tryptophan 199 changed to Alanine,and Proline 202…

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2xFlag CMV2-YAP2-2nd WW mutant

    Plasmid

    #19047

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Tryptophan 258 changed to Alanine, and Proline 2…

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

16. IL1RN gRNA2

    Plasmid

    #113131

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-GRK3

    Plasmid

    #32689

    Depositor

    Robert Lefkowitz

    Article

    Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

    Insert

    GRK3 (GRK3 Bovine)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-N-mSOG-FRB-IRES-mCherry

    Plasmid

    #136602

    Purpose

    Encodes FRB tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

    Depositor

    John Ngo

    Article

    Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

    Insert

    N-mSOG-FRB
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJA285= pSin-TCRab-CMVp-Puro

    Plasmid

    #116875

    Purpose

    stable TCR expression in human T cells

    Depositor

    Marcos Alcocer

    Article

    Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

    Insert

    TCR (TRAV10, TRBV25-1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    deletion of EF1-a promotor

    Promoter

    CMV Promotor

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5 bovine GRK5

    Plasmid

    #14690

    Depositor

    Robert Lefkowitz

    Article

    Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

    Insert

    GRK5 (GRK5 Bovine)
    

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only