We narrowed to 4,434 results for: 1-Oct;

1. pSP64-hERG1a T623A

   Plasmid

   #53055

   Purpose

   Expresses alpha subunit of T623A hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Mutation

   changed Threonine 623 to Alanine

   Promoter

   SP6

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSP64-hERG1a S624A

   Plasmid

   #53056

   Purpose

   Expresses alpha subunit of S624A hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Mutation

   changed Serine 624 to Alanine

   Promoter

   SP6

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

   Plasmid

   #171089

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Decoy sgRNA
   dCas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3X FLAG and Nuclear Localization Signal

   Mutation

   D10A, H840A

   Promoter

   TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPN062

   Plasmid

   #91595

   Purpose

   Express sgRNA targeting human DFNA5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DFNA5 (GSDME Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPN063

   Plasmid

   #91596

   Purpose

   Express sgRNA targeting human DFNA5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DFNA5 (GSDME Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. J-HARS-4

   Plasmid

   #166529

   Purpose

   scFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-length

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. L-SARS-7

   Plasmid

   #166527

   Purpose

   scFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-length

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. L-KARS-5

   Plasmid

   #166530

   Purpose

   scFv of a human scaffold targeting Lysyl-tRNA synthetase. Antigen coverage aa 1-597 of 597, full-length

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CAG-FLEX-HA-DLX2-T2A-mCherry

   Plasmid

   #178585

   Purpose

   Cre-dependent expression of HA-DLX2 and mCherry under the constitutive promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   DLX2 (DLX2 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid

    #185791

    Purpose

    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-sfGFP(S45C)-ZdK1
    

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Ser to Cys mutation at start of GFP, residue 45 i…

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-SOCS6-WT

    Plasmid

    #74496

    Purpose

    Overexpression of SOCS6 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

    Insert

    SOCS6 (Socs6 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    unknown

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pHR-mCherry-p2A-LIC1 Y101A, Y103A

    Plasmid

    #74605

    Purpose

    expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Y101A, Y103A

    Promoter

    SFFV

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-TPD52

    Plasmid

    #172452

    Purpose

    Expression of Tumor Protein D52 (TPD52) with N-terminal FLAG tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD52 (TPD52 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. O-MARS-11

    Plasmid

    #166541

    Purpose

    scFv of a human scaffold targeting Methionyl-tRNA synthetase. Antigen coverage aa 1-225 of 900

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-Prom1 D-5

    Plasmid

    #184017

    Purpose

    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.

    Depositor

    Peter Stoilov

    Article

    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

    Insert

    Prom1 SV8(-Ex19a) D-5 (Prom1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of exon 19a (amino acids 696-701), and d…

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRE-FDXR mut

    Plasmid

    #16611

    Depositor

    Bert Vogelstein

    Article

    Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

    Insert

    FDXR (FDXR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion mutant, contains aa 1-42

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

17. L-NARS-2

    Plasmid

    #166532

    Purpose

    scFv of a human scaffold targeting Asparaginyl-tRNA synthetase. Antigen coverage aa 1-458 of 458, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. O-AARS-51

    Plasmid

    #166539

    Purpose

    scFv of a human scaffold targeting Alanyl-tRNA synthetase. Antigen coverage aa 1-455 of 968

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. L-WARS-26

    Plasmid

    #166528

    Purpose

    scFv of a human scaffold targeting Tryptophanyl-tRNA synthetase. Antigen coverage aa 1-1471 of 471, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_NCK1-2/3

    Plasmid

    #91420

    Purpose

    Protein expression and purification of human SH3 domain construct NCK1-2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCK1-2/3 (NCK1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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