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CPH3487 (YCp LEU2 Rpb1 S1493A)

Plasmid

#91809

Purpose

Yeast expression of S. cerevisiae Rpb1 with a S1493A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed serine 1493 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3488 (YCp LEU2 Rpb1 P1494A)

Plasmid

#91810

Purpose

Yeast expression of S. cerevisiae Rpb1 with a P1494A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed proline 1494 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3508 (YCp LEU2 Rpb1 Y1473A)

Plasmid

#91811

Purpose

Yeast expression of S. cerevisiae Rpb1 with a Y1473A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed tyrosine 1473 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3509 (YCp LEU2 Rpb1 T1471A)

Plasmid

#91812

Purpose

Yeast expression of S. cerevisiae Rpb1 with a T1471A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed threonine 1471 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3511 (YCp LEU2 Rpb1 P1472A)

Plasmid

#91813

Purpose

Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed proline 1472 to alanine

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3567 (pET151_Spt6 1247-1451 R1282H)

Plasmid

#91816

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

changed arginine 1282 to histidine; deleted resid…

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3495 (pET151_Spt6 1247-1451 K1435A)

Plasmid

#91817

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

changed lysine 1435 to alanine; deleted residues …

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P RTEL1_1

Plasmid

#160799

Purpose

Suppress RTEL1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shRTEL1_1

Use

Lentiviral

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1B CDC25A_2

Plasmid

#160768

Purpose

Suppress CDC25A

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25A_2

Use

Lentiviral

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P RTEL1_2

Plasmid

#160800

Purpose

Suppress RTEL1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shRTEL1_2

Use

Lentiviral

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P PRIM2_2

Plasmid

#160796

Purpose

Suppress PRIM2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPRIM2_2

Use

Lentiviral

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P PRIM2_1

Plasmid

#160795

Purpose

Suppress PRIM2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPRIM2_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P PPAT_2

Plasmid

#160794

Purpose

Suppress PPAT

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPPAT_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P PPAT_1

Plasmid

#160793

Purpose

Suppress PPAT

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPPAT_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P NTHL1_2

Plasmid

#160788

Purpose

Suppress NTHL1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shNTHL1_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P NTHL1_1

Plasmid

#160787

Purpose

Suppress NTHL1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shNTHL1_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P EXO5_2

Plasmid

#160784

Purpose

Suppress EXO5

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shEXO5_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P EXO5_1

Plasmid

#160783

Purpose

Suppress EXO5

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shEXO5_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1P ERCC2_2

Plasmid

#160782

Purpose

Suppress ERCC2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shERCC2_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P ERCC2_1

Plasmid

#160781

Purpose

Suppress ERCC2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shERCC2_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1P BRIP1_2

Plasmid

#160772

Purpose

Suppress BRIP1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shBRIP1_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1P BRIP1_1

Plasmid

#160771

Purpose

Suppress BRIP1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shBRIP1_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1B CDC25C_2

Plasmid

#160770

Purpose

Suppress CDC25C

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25C_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1B CDC25C_1

Plasmid

#160769

Purpose

Suppress CDC25C

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25C_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1B CDC25A_1

Plasmid

#160767

Purpose

Suppress CDC25A

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25A_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM33

Plasmid

#154167

Purpose

Aedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Rel2 (long isoform)

Tags

FLAG

Expression

Insect

Mutation

C-terminal IκB domain deleted

Promoter

IE1 promoter and hr5 enhancer from Autographa cal…

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Mm_Plk5(D168A)

Plasmid

#136318

Purpose

Mammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (Plk5 Mouse)

Expression

Mammalian

Mutation

D168A

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Hu_Plk5(W207F)

Plasmid

#136320

Purpose

Mammalian expression of human Plk5 W207F with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (PLK5 Human)

Expression

Mammalian

Mutation

W207F

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONRG_P4-P1R:TBA2p

Plasmid

#128429

Purpose

Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.

Depositor

George Haughn

Article

McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

Insert

TESTA ABUNDANT 2 (AT1G62060 Mustard Weed)

Use

Gateway promoter entry clone

Promoter

TESTA ABUNDANT 2

Available Since

Oct. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
PCRII apoBb.1

Plasmid

#121898

Purpose

Plasmid for making zebrafish apoBb.1 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoBb.1 (apobb.1 Zebrafish)

Use

Unspecified

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PCRII apoA-IVb.1

Plasmid

#121903

Purpose

Plasmid for making zebrafish apoA-IVb.1 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

Apoa4b.1 (apoa4b.1 Zebrafish)

Use

Unspecified

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PCRII apoA-IVb.2

Plasmid

#121904

Purpose

Plasmid for making zebrafish apoA-IVb.2 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

Apoa4b.2 (apoa4b.2 Zebrafish)

Use

Unspecified

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Dynamin1 R290A D291A T292A

Plasmid

#112103

Purpose

Mammalian expression of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R290A D291A T292A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Dynamin1 D406A M407A

Plasmid

#112107

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406A M407A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Dynamin1 D406A M407A F409A

Plasmid

#112108

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406A M407A F409A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Dynamin1 D406R M407R

Plasmid

#112109

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Dynamin1 D406R M407R F409A

Plasmid

#112110

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R F409A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS2235

Plasmid

#111888

Purpose

High copy number plasmid containing Ple320 (POU4F1 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple320 (POU4F1 Human)

Use

Unspecified

Promoter

Ple320 (POU4F1 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
LLP338 pEF1a-DACH1-1-SunTag

Plasmid

#100942

Purpose

TALE vector with DACH1-1 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE target sequence for DACH1, SunTag array (10xGCN4)

Use

TALEN

Tags

3xHA

Expression

Mammalian

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
YCe2741 HC_Kan_EF1ap_p18

Plasmid

#100698

Purpose

part designed to occupy position 18 of EMMA. Functional category: Constitutional promoter

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EF1a promoter (EEF1A1 Human)

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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