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pFTK010

Plasmid

#171282

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PxlnA ANIA_03613 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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FB407

Plasmid

#203628

Purpose

Promoter of the elongation factor 1-alpha gene from P. digitatum (PDIG_59570).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

Pef1A

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-2A-TurboGFP-PEST-PuroR

Plasmid

#80956

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TurboGFP-PEST

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR APLP1 shRNA 2

Plasmid

#30157

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP1 shRNA 2

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pFTK048

Plasmid

#171320

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

DsRed.T1-SKL fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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myc-hULK2

Plasmid

#31966

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK2 (ULK2 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pDHt-SK

Plasmid

#63615

Purpose

The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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myc-mULK1M92A

Plasmid

#31962

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (Ulk1 Mouse)

Tags

myc

Expression

Mammalian

Mutation

M92A

Promoter

CMV, SP6

Available Since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only
pCAX APP C60

Plasmid

#30152

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein C60

Expression

Mammalian

Mutation

fragment

Available Since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only
pCAS9-mCherry-TUBB

Plasmid

#66942

Purpose

CRISPaint target selector TUBB

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA TUBB

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pFTK055

Plasmid

#171327

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

rTA2S-M2 (TetR-3xVP16) transcriptional activator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-ACTG1

Plasmid

#66943

Purpose

CRISPaint target selector ACTG1

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA ACTG1

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 2

Plasmid

#30160

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP2 shRNA 2

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pFTK004

Plasmid

#171276

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PgpdA ANIA_08041 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK020

Plasmid

#171292

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PalcA syn_NoCrea (ANIA_08979) promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-HIST1H4C

Plasmid

#66944

Purpose

CRISPaint target selector HIST1H4C

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA HIST1H4C

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2+-rasmKate

Plasmid

#105938

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Weber et al Cell Rep. 2014 Apr 24;7(2):386-97. doi:

Insert

Ras

Tags

mKate

Expression

Bacterial

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFTK036

Plasmid

#171308

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

amdS ANIA_08777 acetamidase, selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK043

Plasmid

#171315

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

eGFP fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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TAL2369

Plasmid

#36780

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

NCOR1-TALEN_Right (Ncor1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pFTK046

Plasmid

#171318

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

DsRed.T1 fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Cx55.5:rasGFP

Plasmid

#105939

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Weber et al Cell Rep. 2014 Apr 24;7(2):386-97. doi:

Insert

Ras

Tags

GFP

Expression

Bacterial

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFTK056

Plasmid

#171328

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

SpCas9-NLS

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK019

Plasmid

#171291

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PTET-10x-repeat promoter+CPgpdA (fused)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK017

Plasmid

#171289

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PTET-5x-repeat promoter+CPgpdA (fused)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTK038

Plasmid

#171310

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

hph, hygR, hygromycin selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTK037

Plasmid

#171309

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

bleoR phleomycin, selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTK084

Plasmid

#171356

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

TtRNA[Met] A.niger Pol-III terminator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTK039

Plasmid

#171311

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

pyrG AO090011000868 Orotidine 5'-phosphate decarboxylase, selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTK080

Plasmid

#171352

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Tcyc1 YJR048W terminator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge