We narrowed to 10,030 results for: Pol
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Plasmid#193718PurposeExpression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubulesDepositorInserthsKIF5B(1-560)Rigor-L-2xHA (KIF5B Human, Synthetic)
Tags2xHAExpressionMammalianMutationhsKIF5B(aa1-560), RIGOR: G234APromoterChicken beta-actinAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT RAD23A
Plasmid#201438PurposeExpresses human RAD23A with an N-terminal FLAG tag in mammalian cellsDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_GR-RE_MLPmin_BC1092-luc2
Plasmid#227112PurposeGlucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092DepositorInsert12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)
Plasmid#140893PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian celDepositorTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE), cha…Available SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRY-Gal∆DD (B1013)
Plasmid#92035PurposeExpresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRESDepositorInsertCRY2 (CRY2 Mustard Weed)
ExpressionMammalianMutationFusion of plant CRY2 to Gal4 binding domain (AA1-…Available SinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-AktDN
Plasmid#53597Purposelentiviral expression of dominant negative mouse Akt1 and EGFPDepositorInsertAkt1 (Akt1 Mouse)
UseLentiviralTagsHA and IRES-EGFPExpressionMammalianMutationDominant negative (K179M)PromoterCMVAvailable SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP
Plasmid#216475Purposelentiviral dual expression of YAP5SA and eGFPDepositorInsertsYAP5SA
eGFP
UseLentiviralMutationS61A, S109A, S127A, S164A, S381APromoterEF1aL and UBCAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
mouse b3 full length
Plasmid#217814PurposeFull length mouse integrin b3 expressionDepositorAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]
Plasmid#74102Purposeplasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)DepositorInsertsXenopus laevis beta-tubulin gene promoter region and 5'UTR
Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR
ExpressionBacterialAvailable SinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUb-Cas9-EGFP_cU6:6-sgRNA
Plasmid#190597PurposeThe plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.DepositorInsertsCas9
sgRNA
UseCRISPRTagsEGFP - separated by T2APromoterAedes aegypti polyubiquitin and Culex quinquefasc…Available SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNK5664
Plasmid#219747PurposeDVK_AF CIDAR vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of CMV promoter, for mammalian expressionDepositorInsertpCMV - nnLuz_v4 - tSV40
UseLuciferaseExpressionMammalianAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-353)GCN4-L-GFP
Plasmid#193714PurposeExpression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)DepositorInserthsKIF5B(1-353)-GCN4-L-GFP (KIF5B Human, Synthetic)
TagsEGFPExpressionMammalianMutationhsKIF5B(aa1-353);PromoterChicken beta-actinAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delBRCT
Plasmid#173938PurposeBacterial expression of PARP1 lacking BRCT domainDepositorInsertPARP1delBRCT (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 374-520; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Zn2
Plasmid#174805PurposeBacterial expression of isolated PARP1 Zn2 domainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Zn3
Plasmid#174806PurposeBacterial expression of isolated PARP1 Zn3 domainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
CIB-VP64 (B1016)
Plasmid#92037PurposeExpresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)DepositorInsertCIB1-VP64 (CIB1 Mustard Weed)
TagsFusion of plant CIB1 with VP64 activation domain.…ExpressionMammalianPromoterCMVAvailable SinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Frt-hspB3
Plasmid#63094PurposeExpression of HSPB3 (small HSP) in mammalian cellsDepositorAvailable SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Cdt1 human
Plasmid#72681Purposemammalian expression of human Cdt1DepositorAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiDP1.3
Plasmid#171096Purposelentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Cdt1 (AKA ROLECCS G1) and miniAID-fused mCherry2DepositorInsertOsTIR1-mEmerald-Cdt1-P2A-miniAID-mCherry2
UseLentiviral and Synthetic BiologyTagsEmerald, cdt1, miniAID, mCherry2ExpressionMammalianPromoterCMVAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-UtrCH
Plasmid#124656PurposeF-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).DepositorAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-MS2V5-eIF4G delN83
Plasmid#65812Purposeexpression of eIF4G-MS2 fusion.DepositorInserteIF4G (EIF4G1 Human)
TagsMS2 stem loops and V5ExpressionMammalianMutationdelN83 (functionally behaves as the WT protein)PromoterCMVAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiDP1.2
Plasmid#171095Purposelentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2DepositorInsertOsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
UseLentiviral and Synthetic BiologyTagsEmerald, Geminin, miniAID, mCherry2ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_Sik3
Plasmid#119972PurposeLentiviral expression plasmid of mouse Sik3 cDNA with neomycin resistance geneDepositorAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-DTX2-1-178-EGFP-Hygro
Plasmid#238094PurposeExpression of structurally predicted WWE domain of DTX2 with EGFP on C terminalDepositorInsertDTX2 (DTX2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 1-178 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn2
Plasmid#173935PurposeBacterial expression of PARP1 lacking Zn2 domainDepositorInsertPARP1delZn2 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 97-213 (V762A)Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry
Plasmid#122957PurposeLentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expressionDepositorAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-Neo-TetO-Cdk12_Untagged
Plasmid#127178PurposeUntagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vectorDepositorInsertMouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UsePiggybac transposase vectorTagsNoneExpressionMammalianPromoterTetO PromoterAvailable SinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
SaCas9v2-Puro
Plasmid#178802PurposeSaCas9 with 2A-Puro, and a cloning backbone for sgRNA.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available SinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP
Plasmid#216477Purposelentiviral dual expression of dsRed and tagBFP with loxP site for Cre excisionDepositorInsertsdsRed
tagBFP
UseCre/Lox and LentiviralPromoterEF1aL and UBCAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_AR-RE_MLPmin_BC1014-luc2
Plasmid#227108PurposeAndrogen receptor response element for luciferase and barcode assays; barcode BC1014DepositorInsert8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUb-Cas9-mCherry_cU6:6-sgRNA
Plasmid#190598PurposeThe plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoterDepositorInsertsCas9
sgRNA
UseCRISPRTagsmCherry - separated by T2APromoterAedes aegypti polyubiquitin and Culex quinquefasc…Available SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_ER-RE_MLPmin_BC1063-luc2
Plasmid#227109PurposeEstrogen receptor response element for luciferase and barcode assays; barcode BC1063DepositorInsert12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRE-Lepr_Short-IRES-eGFP
Plasmid#196935PurposeDoxycycline inducible expression of short Lepr (no signaling) with eGFP markerDepositorInsertLepr (Lepr Mouse)
UseLentiviralExpressionMammalianMutationshort isoform, lack of signaling casacadePromoterTREAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-mScarlet-Dync1h1motor
Plasmid#191330PurposeExpresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
ExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644Available SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delCAT
Plasmid#173942PurposeBacterial expression of PARP1 lacking CAT domainDepositorInsertPARP1delCAT (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 661-1014Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)
Plasmid#63139PurposeExpression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)DepositorTagsGSTExpressionBacterialMutationResidues 1-109 and Residues 1-159PromotertacAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-lox-3xSTOP-lox (JDW 869)
Plasmid#224521PurposeA Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassetteDepositorInsertLoxP-3xStop-LoxP
UseCre/LoxAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-roxSTOProx-tRFP-pA-lox
Plasmid#196888PurposeDre-activated Cre-inactivated expression of turbo (t)RFP reporter.DepositorInsertturbo (t)RFP
UseCre/Lox; Dre/roxExpressionMammalianPromoterQuimeric CAGAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA
Plasmid#164471PurposeExpresses sgRNA targeting human GJA1 locusDepositorAvailable SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only