We narrowed to 4,107 results for: lenti crispr plasmid

1. EFS-SpdCas9

   Plasmid

   #210703

   Purpose

   This plasmid express EFS promoter driven human codon optimized SpdCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   S. Pyogenes dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a core promoter

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLCKO2

   Plasmid

   #125518

   Purpose

   Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.

   Depositor

   Jason Moffat

   Article

   Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW-tTRE-scFv-SunTag

   Plasmid

   #193138

   Purpose

   Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

   Depositor

   Brad Rosenberg

   Article

   Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

   Insert

   scFv-SunTag
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo

   Plasmid

   #239608

   Purpose

   Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistance

   Depositor

   Philipp Rathert

   Article

   Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

   Insert

   filler-trcr-H1-filler
   

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA-Dual_filler(hU6_H1/7SK hybrid)-EF1as_Thy1.1_P2A_Neo

   Plasmid

   #239609

   Purpose

   Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and minimal H1/7SK hybrid promoters) using BsmbI. Selectable with a G418 resistance

   Depositor

   Philipp Rathert

   Article

   Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

   Insert

   filler-trcr-H1/7SKhybrid-filler
   

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

   Plasmid

   #188703

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

   Plasmid

   #164800

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA1
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

   Plasmid

   #164801

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pgRNA-CKB

   Plasmid

   #73501

   Purpose

   gRNA expression vector (with mKate2)

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   mKate2-T2A-Bsd
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid

    #117687

    Purpose

    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

    Depositor

    Erik Sontheimer

    Article

    Gao et al Methods Enzymol. 2019;616:365-383. doi:

    Inserts

    Kanamycin cassette
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV U6-sgRNA UbC-GFP

    Plasmid

    #106948

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ABE8e SpG Puro

    Plasmid

    #235044

    Purpose

    Adenosine base editor 8e* with SpG nicking Cas9 makes A > G edits

    Depositor

    Justin Pritchard

    Article

    Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

    Insert

    ABE8e, nCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-PuroR

    Promoter

    EF1a core

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid

    #85452

    Purpose

    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

    Depositor

    Aviv Regev

    Article

    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

    Insert

    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. LCV2_LacZ_sgRNA_2

    Plasmid

    #155093

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    LacZ_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LCV2_Luciferase_sgRNA

    Plasmid

    #155094

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting Luciferase

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    Luciferase_sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pgRNA-modified

    Plasmid

    #84477

    Purpose

    guide RNA expression lentiviral construct

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    guide RNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSaCas9-sgRNA-Tetra-com vector

    Plasmid

    #131227

    Purpose

    For expressing gRNA, whose Tetraloop is replaced with a com aptamer

    Depositor

    Baisong Lu

    Article

    Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

    Insert

    vector for com modified sgRNA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-PGK-scFv GCN4-sfGFP-VP64

    Plasmid

    #122133

    Purpose

    Express a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    scFv GCN4-sfGFP-VP64
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

    Plasmid

    #234882

    Purpose

    L1HS-silencing plasmid (CRISPRi gRNA1)

    Depositor

    Johan Jakobsson

    Article

    Adami et al bioRxiv 2025.01.17.633315

    Insert

    L1HS-silencing plasmid (CRISPRi gRNA1)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Pro-Code Vector Kit

    Plasmid Kit

    #1000000177

    Purpose

    The Pro-Code Vector Kit is a set of 120 lentiviral vector plasmids, each with a unique protein barcode (Pro-Code) and gRNA cloning site.These plasmids can be used for CRISPR screens.

    Depositor

    Brian Brown

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only