We narrowed to 26,505 results for: vit;

1. HMA-APOBEC1-Nb

   Plasmid

   #226012

   Purpose

   For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

   Depositor

   Eugene Yeo

   Article

   Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Tags

   His-MBP and antiRabbit-nanobody

   Expression

   Bacterial

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmROSA26-attP50-Neo-mKate2-3xNLS-attP50

   Plasmid

   #183609

   Purpose

   Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Inserts

   Neo
   mKate2-3xNLS
   

   Use

   Mouse Targeting

   Tags

   3xNLS

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T-1 3xFlag-JNK1a1

   Plasmid

   #47574

   Purpose

   Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   JNK1a1 (MAPK8 Human)
   

   Tags

   3xFlag and GST

   Expression

   Bacterial

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCDH-SIRT2-Flag

   Plasmid

   #102624

   Purpose

   Expresses full length SIRT2 isoform 1 in mammalian cells

   Depositor

   Hening Lin

   Article

   Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

   Insert

   SIRT2 (SIRT2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLR5-CAG-44B12mut23(Ser5ph)-SNAP

   Plasmid

   #174877

   Purpose

   PiggyBac transposon vector for expression of RNAPII Ser5ph mintbody

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   RNAPII Ser5ph mintbody-SNAP
   

   Use

   Piggybac vector

   Expression

   Mammalian

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CaV2.1/pcDNA3.1 ~ Human

   Plasmid

   #140575

   Purpose

   Expression in mammallian cells or oocytes

   Depositor

   Dan Minor

   Article

   Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

   Insert

   CaV2.1 Human (CACNA1A Human)
   

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1-Tet-UPF1WT (pRKB264)

   Plasmid

   #99146

   Purpose

   Inducible lentiviral construct to express FLAG-tagged UPF1wt

   Depositor

   Robert Bradley

   Article

   Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   First 127 codons are optimized to reduce GC conte…

   Promoter

   pTight

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

   Plasmid

   #84471

   Purpose

   pAAV-RAM-ChR2

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   Channelrhodopsin 2
   

   Use

   AAV

   Tags

   EYFP

   Mutation

   H134R

   Promoter

   TRE

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiPGK Hygro DEST ERKKTRmCerulean3

   Plasmid

   #90229

   Purpose

   Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA WDR24 pRK5

    Plasmid

    #46335

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR24 (WDR24 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. p206-Switch-ON

    Plasmid

    #217885

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204341

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Galpha-i3/Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pX458-Ef1a-Cas9-H2B-mCherry

    Plasmid

    #159655

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIB-FLAG(N)-RPB1

    Plasmid

    #231722

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    POLR2A (POLR2A Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-REPORT(EF1a)

    Plasmid

    #172326

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

    Plasmid

    #233080

    Purpose

    Expression vector for sgRNA-S1b and eCas12f1i

    Depositor

    Kyoungmi Kim

    Article

    Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

    Insert

    U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xFLAG

    Expression

    Mammalian

    Promoter

    hU6, CBh

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICE-HA-MRE11-WT

    Plasmid

    #82033

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA

    Plasmid

    #84474

    Purpose

    pAAV-RAM-NLS-mKate2

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    mKate2
    

    Use

    AAV

    Tags

    Nuclear localization signal of SV40 large T antig…

    Promoter

    TRE

    Available Since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHW1385

    Plasmid

    #229879

    Purpose

    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    

    Tags

    mScarlet-I-C1

    Expression

    Worm

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. Sniper2L

    Plasmid

    #193856

    Purpose

    Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

    Insert

    Sniper2L
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    E1007L

    Promoter

    EFS

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a

    Plasmid

    #233079

    Purpose

    Expression vector for sgRNA-S1b and eCas12f1a

    Depositor

    Kyoungmi Kim

    Article

    Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

    Insert

    U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xFLAG

    Expression

    Mammalian

    Promoter

    hU6, CBh

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. SpCas9 TadDE

    Plasmid

    #193837

    Purpose

    Express TadDE (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-DualEditor-Cas9D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HA Sec13 pRK5

    Plasmid

    #46332

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Sec13 (SEC13 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Sumo3 PolQM1

    Plasmid

    #78462

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie, Susan Wallace

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; Q2513R (please see depositor commen…

    Promoter

    T7

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pCMV3Tag8li-hHP1BP3-HA

    Plasmid

    #165468

    Purpose

    Human HP1BP3 mammalian expression vector with C-terminal HA tag.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    Heterochromatin protein 1 binding protein 3 (HP1BP3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLVX-CMV-Galpha-i3 ONE-GO

    Plasmid

    #189737

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-i3 ONE-GO
    GNAI3 (Gnai3 Rat)
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. pTight-hASCL1-N174

    Plasmid

    #31876

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    achaete-scute complex homolog 1 (ASCL1 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. STARR-seq luciferase validation vector_ORI_empty

    Plasmid

    #99297

    Purpose

    Luciferase validation vector; empty vector without insert to determine baseline

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Luciferase; Starr-seq luciferase validation vector

    Expression

    Mammalian

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAV-Puro_siKD

    Plasmid

    #86695

    Purpose

    Tetracycline inducible expression of shRNA targeted to AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    H1 TO

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDM304_DPAKa(GBD)-YFP

    Plasmid

    #128699

    Purpose

    expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

    Depositor

    Igor Weber

    Article

    Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

    Insert

    GBD domain (aa 731-890) of DPAKa
    

    Use

    Dictyostelium discoideum expression vector

    Tags

    YFP

    Mutation

    none

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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32. pINDUCER11 (miR-RUG)

    Plasmid

    #44363

    Purpose

    Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

    Depositor

    Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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33. pDM358_DPAKa(GBD)-YFP

    Plasmid

    #128700

    Purpose

    expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

    Depositor

    Igor Weber

    Article

    Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

    Insert

    GBD domain (aa 731-890) of DPAKa
    

    Use

    Dictyostelium discoideum expression vector

    Tags

    YFP

    Mutation

    none

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCS2+8-CYP2A6-flag-tev-his

    Plasmid

    #227165

    Purpose

    For overexpression of CYP2A6 in mammalian cells and purification.

    Depositor

    Yimon Aye

    Article

    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

    Insert

    CYP2A6 (CYP2A6 Human)
    

    Tags

    Flag and His6

    Expression

    Mammalian

    Promoter

    CMV and SP6

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDM326_DPAKa(GBD)-YFP

    Plasmid

    #128701

    Purpose

    expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

    Depositor

    Igor Weber

    Article

    Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

    Insert

    GBD domain (aa 731-890) of DPAKa
    

    Use

    Dictyostelium discoideum expression vector

    Tags

    YFP

    Mutation

    none

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGEMHE-mEGFP-Map4

    Plasmid

    #105571

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    MAP4 (Map4 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA

    Plasmid

    #63931

    Purpose

    Empty AAV-RAM plasmid w. multiple cloning site (MCS)

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Type

    Empty backbone

    Use

    AAV

    Promoter

    pRAM and TRE

    Available Since

    Dec. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCuo CA Rac1

    Plasmid

    #84643

    Purpose

    constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.

    Depositor

    Sanjay Kumar

    Article

    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Constitutively Active, Q61L

    Promoter

    cumate-inducible promoter

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. xCas9

    Plasmid

    #138565

    Purpose

    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    xCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pT3TS nErCas12an

    Plasmid

    #132642

    Purpose

    Used for in vitro transcription of dual NLS ErCas12a mRNA

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    CRISPR; In vitro transcription

    Tags

    SV40 NLS

    Promoter

    T3

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge