We narrowed to 4,107 results for: lenti crispr plasmid

1. gRNA-10Xcapture-PuroR

   Plasmid

   #211496

   Purpose

   Plasmid for the expression of SAM-compatible gRNA for CRISPRa with capturer sequence for 10X Genomics sequencing

   Depositor

   Lesley Forrester, Antonella Fidanza

   Article

   Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

   Inserts

   U6-gRNA
   Ef1a-PuroR
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a and U6

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDA_886

   Plasmid

   #201162

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_887

   Plasmid

   #201164

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_888

   Plasmid

   #201165

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nme2sgRNA_pLKO

   Plasmid

   #119926

   Purpose

   Nme2Cas9 U6-driven sgRNA cassette

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   NmeCas9 sgRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pL_FR_Hygro

   Plasmid

   #127171

   Purpose

   Frameshift reporter (control plasmid for screening)

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Insert

   Frameshift Reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ5079_pHR_PGK_sfGFP_CoV-F1

   Plasmid

   #155303

   Purpose

   SARS-CoV-2 fluorescent reporter 1

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Superfolder GFP fused with SARS-CoV-F1 (ORF1ab Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LCV2_AAVS1_sgRNA_2

   Plasmid

   #155088

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LCV2_AAVS1_sgRNA_3

   Plasmid

   #155089

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_3
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LCV2_AAVS1_sgRNA_4

    Plasmid

    #155090

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_4
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. LCV2_PSMB2_sgRNA_1

    Plasmid

    #155092

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting PSMB2 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMB2_sgRNA_1 (PSMB2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. LCV2_PSMD1_sgRNA_1

    Plasmid

    #155091

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting PSMD1 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMD1_sgRNA_1 (PSMD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLEX307_CR-PDHA2S291D

    Plasmid

    #115200

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S291D (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX307_CR-PDHA2S293D

    Plasmid

    #115201

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX307_CR-PDHA2S291D/S293D

    Plasmid

    #115202

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S291D/S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_052

    Plasmid

    #136474

    Purpose

    Lentiviral expression of AsCas12a gRNA

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    Puromycin resistance
    

    Use

    Lentiviral and Synthetic Biology

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(hMTOR_1k)-PGKpuro2ABFP-W

    Plasmid

    #208429

    Purpose

    Lentiviral gRNA plasmid targeting human MTOR gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

    Insert

    MTOR (MTOR Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    human U6

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNA5(hMTOR_2b)-PGKpuro2ABFP-W

    Plasmid

    #208430

    Purpose

    Lentiviral gRNA plasmid targeting human MTOR gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

    Insert

    MTOR (MTOR Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    human U6

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAT001

    Plasmid

    #171061

    Purpose

    Plasmid for expressing HIV-1 envelope glycoprotein

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Insert

    HIV-1 Env
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. scFv-GCN4-sfGFP-deadTET1CD

    Plasmid

    #184442

    Purpose

    Catalytically DEAD TET1CD with scFv against GCN4

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    Catalytically DEAD TET1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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