We narrowed to 26,505 results for: vit;

1. LentiCRISPRv2hyg-Ptgs1

   Plasmid

   #170299

   Purpose

   A knock-out vector for the mouse Ptgs1

   Depositor

   Michiyuki Matsuda

   Article

   Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

   Insert

   A gRNA targeting the mouse Ptgs1 gene.
   

   Use

   CRISPR and Lentiviral

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_SC_4.26_STARR-seq

   Plasmid

   #220220

   Purpose

   AAV-STARR-seq screening vector with 4.26 promote

   Depositor

   Simon Rumpel

   Article

   Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Promoter

   4.26

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. NPY1R-DuET

   Plasmid

   #213354

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   NPY1R (NPY1R Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P-2-human RNF146 full length

   Plasmid

   #132610

   Purpose

   expresses human RNF146 full length in E. coli

   Depositor

   Wenqing Xu

   Article

   DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

   Insert

   RNF146 (RNF146 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-hUba2-His

   Plasmid

   #53117

   Purpose

   expression of full length human Uba2 with Cterminal His-tag in E.coli

   Depositor

   Frauke Melchior

   Article

   Werner et al Methods Mol Biol. 2009;497:187-99. doi:

   Insert

   UBA2 (UBA2 Human)
   

   Tags

   His and T7 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-GIT1

   Plasmid

   #15226

   Depositor

   Rick Horwitz

   Article

   Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

   Insert

   G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   L425V, E476K and L583P

   Available Since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. HypaCas9

   Plasmid

   #138557

   Purpose

   Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   HypaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP gRNA (BRDN0000561167)

   Plasmid

   #80034

   Purpose

   3rd generation lentiviral gRNA plasmid targeting EGFP

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. KRas FAK biosensor

   Plasmid

   #78301

   Purpose

   FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   KRas FAK biosensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pET21a-C2-SNAP

    Plasmid

    #208769

    Purpose

    To express C2-SNAP in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2-SNAP (Mfge8 Mouse)
    

    Tags

    SNAP-tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. hHSF1 K80Q

    Plasmid

    #118351

    Purpose

    Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

    Depositor

    Lea Sistonen

    Article

    Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K80Q

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHis6-hDF

    Plasmid

    #72585

    Purpose

    human dysferlin full length tagged with His

    Depositor

    Renzhi Han

    Article

    Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

    Insert

    dysferlin (DYSF Human)
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-RAM-d2TTA::TRE-ArchT-WPREpA

    Plasmid

    #84472

    Purpose

    pAAV-RAM-ArchT

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    ArchT
    

    Use

    AAV

    Tags

    EGFP

    Promoter

    TRE

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Syn-SomArchon

    Plasmid

    #126941

    Purpose

    Expression of SomArchon under Syn promoter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Myc-Bnip3FL

    Plasmid

    #100796

    Purpose

    Mammalian expression of Bnip3 full length splice variant

    Depositor

    Joseph Gordon

    Article

    Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

    Insert

    mus Bnip3 (Bnip3 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6 SgRNA RBM20

    Plasmid

    #162735

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    Spacer sequence for RBM20
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRN3P-HA-Suv4-20h1

    Plasmid

    #86689

    Purpose

    For transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5B (Kmt5b Mouse)
    

    Tags

    HA-tag

    Promoter

    T3

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV2_CAG_oROS-G

    Plasmid

    #216115

    Purpose

    Encodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCardinal-Lysosomes-20

    Plasmid

    #56166

    Purpose

    Localization: Lysosome Membrane, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    LAMP1
    

    Tags

    mCardinal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pWPXL-Sox4

    Plasmid

    #36984

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    Sox4 (SOX4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMIA4.721-Keppel

    Plasmid

    #214444

    Purpose

    BxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    2xFKBP12-Casp9
    

    Use

    Hdr donor

    Tags

    P2A-Bsd

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2

    Plasmid

    #112004

    Purpose

    For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV9

    Insert

    axon-GCaMP6s-P2A-mKate2
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Promoter

    hSynapsin1

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pMSCV-GSDMD-Myc

    Plasmid

    #201166

    Purpose

    Expression of GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Promoter

    MESV

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. PB-Hyg-Dest-mCherry-PAR-6B

    Plasmid

    #194908

    Purpose

    To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

    Depositor

    Clare Buckley

    Article

    Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

    Insert

    PAR-6B (Pard6b Mouse)
    

    Use

    Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    39 C to G, silent mutation

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCAGGS puro human-GFP-RLIM-IRES-mCherry

    Plasmid

    #232243

    Purpose

    Mammalian expression of RLIM-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    RLIM-GFP-IRES-mCherry (RLIM Human)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SSTR3-DuET

    Plasmid

    #213378

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR3 (SSTR3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HaloTag-3xrGBD

    Plasmid

    #176108

    Purpose

    Visualization of active Rho

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    rhotekin (Rtkn Mouse)
    

    Expression

    Mammalian

    Promoter

    CMVdel

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

    Plasmid

    #12153

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Retroviral

    Tags

    6XHis

    Expression

    Mammalian

    Available Since

    July 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHAGE-FLAG-APEX2-LGALS8

    Plasmid

    #175758

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS8 (LGALS8 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4

    Plasmid

    #177265

    Purpose

    STREAMING-tag cassette

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag cassette
    

    Use

    Unspecified

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-myc-FLNa S2152A

    Plasmid

    #8983

    Depositor

    John Blenis

    Article

    Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

    Insert

    Filamin A S2152A (FLNA Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Ser2152Ala

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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32. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid

    #75154

    Purpose

    Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    H2B-mCerulean3-P2A-mKOFP2-CAAX
    

    Use

    Middle entry vector for multisite gateway three f…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. OPN3-DuET

    Plasmid

    #213357

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPN3 (OPN3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204340

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Galpha-s/Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

    Plasmid

    #183606

    Purpose

    Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    EGFP-TM
    

    Tags

    HA, Human PDGFRB transmembrane domain, Mouse IgGK…

    Expression

    Mammalian

    Promoter

    Mouse Pgk1

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTriEx-RhoA FLARE.sc Biosensor Q63L

    Plasmid

    #12151

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor Q63L (RHOA Human)
    

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    RhoA Q63L (dominant-positive mutation)

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET21a Ov-iPGM

    Plasmid

    #180245

    Purpose

    Bacterial expression plasmid for production of recombinant Onchocerca volvulus iPGM His10

    Depositor

    James Inglese

    Article

    Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

    Insert

    Onchocerca volvulus iPGM-10His
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET21a Ce-iPGM-bio

    Plasmid

    #162559

    Purpose

    Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

    Depositor

    James Inglese

    Article

    Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

    Insert

    C. elegans iPGM bio-6His (ipgm-1 Nematode)
    

    Tags

    6His tag, T7 tag, Thrombin cleavage site, and bio…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET21a Bm-iPGM-bio

    Plasmid

    #162565

    Purpose

    Expresses B. malayi iPGM containing a C-terminal biotinylation site and His tag

    Depositor

    James Inglese

    Article

    Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

    Insert

    B. malayi iPGM bio-6His
    

    Tags

    6His tag, T7 tag, biotinylation sequence, and thr…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAc5.1-V5-HisB-ObKHC-wt-HaloTag

    Plasmid

    #201551

    Purpose

    Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

    Depositor

    Joshua Rosenthal

    Article

    Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

    Insert

    Kinesin-1 (partial)
    

    Tags

    HaloTag

    Expression

    Insect

    Mutation

    Codon-optimized for Drosophila expression

    Promoter

    Ac5

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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