We narrowed to 30,224 results for: vit;

1. GIT1A-FLAG

   Plasmid

   #15225

   Depositor

   Rick Horwitz

   Article

   Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

   Insert

   G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Rec-PhoA/CsgA

   Plasmid

   #170787

   Purpose

   Recombinase controlled expression of csgA and phoA. Expression is activated upon flipping of inverted proD with corresponding recombinase

   Depositor

   Urartu Seker

   Article

   Ahan et al Chembiochem. 2019 Jul 15;20(14):1799-180

   Inserts

   csgA (csgA )
   phoA (phoA )
   

   Use

   Synthetic Biology

   Tags

   HHHHHH

   Expression

   Bacterial

   Promoter

   Inverted proD with Bxb1recognition sites and Inve…

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AB.pCCL.sin.cPPT.U6.miR-17-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46594

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-17 Decoy (MIR17, Mir17 Mouse, Human)
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

4. MTNR1A-DuET

   Plasmid

   #213348

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   MTNR1A (MTNR1A Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET23a-His-hRanGAP1tail

   Plasmid

   #53139

   Purpose

   E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   RanGAP1 (RANGAP1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   C-terminal fragment (amino acids 398-587)

   Promoter

   T7

   Available Since

   May 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CaMKII-iGECI

   Plasmid

   #160423

   Purpose

   AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pVETL-Cmcs-Flag-Ataxin3Q22-C14A

   Plasmid

   #22119

   Depositor

   Henry Paulson

   Article

   Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C14A

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pHAGE-FLAG-APEX2-CALCOCO2

   Plasmid

   #175759

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-CALCOCO2/NDP52

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   NDP52/CALCOCO2 (CALCOCO2 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-REPORT(PGK/CMV)-GtwyA

   Plasmid

   #208574

   Purpose

   LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   mnucTomato
   HSVtk
   Neomycin
   d2nucEGFP
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   PGK/CMV and minP

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

    Plasmid

    #115789

    Purpose

    Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    NDP (NDP Human)
    

    Tags

    alkaline phosphatase and flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVETL-Cmcs-Flag-Ataxin3Q80-C14A

    Plasmid

    #22121

    Depositor

    Henry Paulson

    Article

    Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C14A

    Available Since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pPBC-LG6f

    Plasmid

    #62808

    Purpose

    pPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-Lck-GCaMP6f-ITR
    

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCyPet-Rac1(T17N)

    Plasmid

    #22784

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    Rac1 (T17N) (RAC1 A. victoria, Human)
    

    Tags

    CyPet

    Expression

    Mammalian

    Mutation

    Rac1 T17N.

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pPBC-G3-tdT

    Plasmid

    #62814

    Purpose

    pPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-GCaMP3-IRES-tdTomato-ITR
    

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEMHE-mCherry-mTrim21ΔRING-Box

    Plasmid

    #105523

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Mutation

    deletion of RING domain (aa124-462)

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5RS-HERCD533

    Plasmid

    #65224

    Purpose

    Expression of dominant negative EGFR in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Redemann et al Mol Cell Biol. 1992 Feb;12(2):491-8.

    Insert

    HERCD533 (EGFR Human)
    

    Expression

    Mammalian

    Mutation

    deleted AA 654-1186

    Promoter

    CMV

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

17. AB.pCCL.sin.cPPT.U6.miR-29b-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46605

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-29b Decoy (MIR29B1, Mir29b-1 Mouse, Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid

    #114111

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

    Insert

    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SpCas9 TadCBEc

    Plasmid

    #193834

    Purpose

    Express TadCBEc (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDc-SpCas9 D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHis6-hDF-deltaTM-pCMV-9

    Plasmid

    #72587

    Purpose

    human dysferlin no transmembrane domain tagged with his tag

    Depositor

    Renzhi Han

    Article

    Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

    Insert

    dysferlin (DYSF Human)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    transmembrane domain deleted

    Promoter

    CMV

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pFBD-Akt1(E17K)

    Plasmid

    #86563

    Purpose

    Expresses human full-length Akt1(E17K) mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    E17K mutation in the PH domain

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-4T-1 3xFlag-ERK2

    Plasmid

    #47573

    Purpose

    Bacterial expression plasmid for GST-3xFlag-tagged ERK2

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    ERK2 (Mapk1 Rat)
    

    Tags

    3xFlag and GST

    Expression

    Bacterial

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204343

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-i3/Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pK1E-RNAP

    Plasmid

    #48144

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    rna polymerase K1E
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBEG R2-dsRed-L3 #BGV203

    Plasmid

    #48973

    Purpose

    Supplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    dsRed
    

    Use

    Gateway cloning vector

    Tags

    IRES (strong)

    Promoter

    none

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pOP/SSAT

    Plasmid

    #140291

    Purpose

    Expresses human SSAT in bacterial cells

    Depositor

    Saverio Bettuzzi, Gaetano Marverti

    Article

    Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

    Insert

    SSAT1 (SAT1 Human)
    

    Expression

    Bacterial

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-4mtD3cpv

    Plasmid

    #181874

    Purpose

    Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    4mtD3cpv
    

    Use

    AAV and Adenoviral

    Expression

    Mammalian

    Mutation

    the 4mtD3cpv CDS differs from the recorded GeneBa…

    Promoter

    CaMKIIa

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRSV-CaMKIV

    Plasmid

    #45062

    Purpose

    Expression of CaMKIV

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    Cam Kinase IV (Camk4 Mouse)
    

    Expression

    Mammalian

    Mutation

    *

    Promoter

    RSV

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET14b-PITPalpha

    Plasmid

    #58256

    Purpose

    bacterial expression of human PITP alpha

    Depositor

    Shamshad Cockcroft

    Article

    Hara et al J Biol Chem. 1997 Jun 6;272(23):14908-13

    Insert

    PITP alpha (PITPNA Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pLenti-eGFP-dSaCas9-blast

    Plasmid

    #191653

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    eGFP-T2A-dSaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

    Plasmid

    #199585

    Purpose

    Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

    Depositor

    Michael Lin

    Article

    Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

    Inserts

    ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
    AkaLuc
    Blasticidin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-H2B-μDpTT

    Plasmid

    #182328

    Purpose

    Histone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    H2B-μDronpa-TagRFP-T (H2BC3 Human)
    

    Tags

    Full-length human histone H2B

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBabe-Ypet-PBD

    Plasmid

    #24533

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    PAK binding domain (PAK1 Human)
    

    Use

    Retroviral

    Tags

    Ypet

    Expression

    Mammalian

    Available Since

    Aug. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pGL4.10-VEGFprom(-500 to -1)

    Plasmid

    #66130

    Purpose

    Luciferase-based reporter for VEGF promoter region (-500 to -1 bp)

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    Vascular Endothelial Growth Factor-A promoter region (VEGFA Human)
    

    Use

    Luciferase

    Promoter

    VEGF -500 to -1

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBEG R2-iBlast-L3 #BGV204

    Plasmid

    #48982

    Purpose

    Supplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    blasticidin-S deaminase
    

    Use

    Gateway cloning vector

    Tags

    IRES (weak)

    Promoter

    na

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pUG2K

    Plasmid

    #35493

    Depositor

    Ramesh Shivdasani

    Article

    Horst et al Cancer Res. 2012 Feb 8.

    Insert

    KRAS(G12V) (KRAS Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Ubiquitin C

    Available Since

    May 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. DN-LOXL2

    Plasmid

    #200062

    Purpose

    LOXL2 deletion mutant lacking 1-338 amino acids

    Depositor

    Lakshmi Santhanam

    Article

    Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

    Insert

    human lysyl oxidase like 2 (LOXL2 Human)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    N-terminus amino acids 1-338 truncated.

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. R338G/V339P-LOXL2

    Plasmid

    #200063

    Purpose

    Point mutant of hLOXL2 resistant to processing by Factor Xa

    Depositor

    Lakshmi Santhanam

    Article

    Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

    Insert

    human lysyl oxidase like 2 (LOXL2 Human)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    S300P mutation

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDNAIIIB cdc42 QL

    Plasmid

    #61636

    Purpose

    mammalian expression of cdc42 constitutively active mutant

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    Cdc42 (CDC42 Human)
    

    Expression

    Mammalian

    Mutation

    Q61L, constitutively active

    Promoter

    CMV

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

40. HsCLYBL-FLAG

    Plasmid

    #111290

    Purpose

    mammalian expression

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    human CLYBL (CLYBL Human)
    

    Tags

    linker and FLAG tag: TGGGSGGSGGSMDYKDDDDK

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge