We narrowed to 16,929 results for: igh

1. pPDS1-mEGFP-IRES-puro2b

   Plasmid

   #26464

   Depositor

   Daniel Gerlich

   Article

   Held et al Nat Methods. 2010 Sep . 7(9):747-54.

   Insert

   pPDS1 (PTTG1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Abp1-tDimerRFP

   Plasmid

   #27699

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Abp1 (Dbnl Mouse)
   

   Use

   Tags

   tDimer-RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pRN3P_T3_BE3_IVT

   Plasmid

   #177015

   Purpose

   Plasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymerase

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

   Insert

   BE3
   

   Use

   CRISPR; Vector for in-vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T3 promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA3.1+ HA-hAO7

   Plasmid

   #137840

   Purpose

   N-terminal tagged human AO7 (aa 2-439) for expression in mammalian cells

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 (RNF25 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mc2 blast EF1a

   Plasmid

   #206223

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Dendra2-Gamma-Tubulin-17

   Plasmid

   #57721

   Purpose

   Localization: Centrosomes, Excitation: 490 / 553, Emission: 507 / 573

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   Gamma-Tubulin (TUBG1 Human)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pORE303N_CEN12RRFuzzy3MYB

   Plasmid

   #194441

   Purpose

   CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

   Depositor

   Chung-Jui Tsai

   Article

   Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

   Insert

   gRNA array targeting CEN1, RR, Fuzzy3MYB
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   MtU6

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR1A_no_ccDB_mUty

   Plasmid

   #158570

   Purpose

   V5-tagged sequence of mUty cloned into entry vector for recombination

   Depositor

   Christian Deschepper

   Article

   Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

   Insert

   Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSLIK 3xFLAG-MTUS1 zeo

   Plasmid

   #192323

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-MTUS1

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   MTUS1 (MTUS1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYFAC

    Plasmid

    #168982

    Purpose

    Filamentous fungi vector. Also referred to as pYFAC-pyrG.

    Depositor

    Yit Heng Chooi

    Article

    Chooi et al Environ Microbiol. 2017 May;19(5):1975-1

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in fil…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NmeCas9_3XNLS_pMCSG7

    Plasmid

    #120078

    Purpose

    Bacterial expression of wtNmeCas9 with 3X NLS

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    NmeCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MXS_PGK::CreERT2-bGHpA

    Plasmid

    #62444

    Purpose

    MXS_chaining vector with PGK::CreERT2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tamoxifen inducible Cre recombinase with PGK Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AB65_pCAG.Cas9-D10A.rBGpA

    Plasmid

    #199256

    Purpose

    Expression construct encoding the SpCas9-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    SpCas9.D10A nickase
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYFAC-CH1

    Plasmid

    #168977

    Purpose

    AMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacI

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in fil…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EmptyTranscriptionalReporter

    Plasmid

    #62453

    Purpose

    MXS_chaining vector with 2 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMVmin::NLS-Citrine-bGHpA
    CMV::NLS-Cherry-bGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21a - APX

    Plasmid

    #89618

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Ascorbate dependent Tryparedoxin peroxidase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRL_yEGFP_hc

    Plasmid

    #98746

    Purpose

    Reporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

    Insert

    Yeast-enhanced green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    synthetic

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SlugBE4max

    Plasmid

    #163799

    Purpose

    Expresses AncBE4max and nSlugCas9

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SlugCas9 (D10A)

    Promoter

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOAD

    Plasmid

    #196825

    Purpose

    Yeast two-hybrid ; Gal4 activation domain

    Depositor

    Stan Fields

    Article

    Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

    Type

    Empty backbone

    Use

    Tags

    Gal4 Activation domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Nano-lantern(Ca2+)_300/pcDNA3

    Plasmid

    #51983

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-3GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MXS_CMV::CreERT2-bGHpA

    Plasmid

    #62443

    Purpose

    MXS_chaining vector with CMV::CreERT2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tamoxifen inducible Cre recombinase with CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mEmerald-MCM6-N-22

    Plasmid

    #54172

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

    Insert

    MCM6
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pWXLd-RIEP-OgNLuc

    Plasmid

    #135936

    Purpose

    Expresses the OgNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    OgNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYPQ-dpcoCas9-EV2.1

    Plasmid

    #158411

    Purpose

    CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-attBr-Cre-rc-attP

    Plasmid

    #51264

    Purpose

    Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    attBr-Cre-rc-attP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mRuby2-MannII-N-10

    Plasmid

    #55903

    Purpose

    Localization: Mannosidase/Golgi, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    MannII
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    aa 1-112 of MannII

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

27. pOVC1_EcSSPB-nano_X

    Plasmid

    #131212

    Purpose

    Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    EcSSPB-nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJK03_Rho_basal_DsRed

    Plasmid

    #173490

    Purpose

    Vector backbone used to clone MPRA libraries

    Depositor

    Barak Cohen

    Article

    White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Insert

    Bovine Rhodopsin promoter driving DsRed fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Bovine Rhodopsin

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mc2 mCherry

    Plasmid

    #206219

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. IDP466

    Plasmid

    #87008

    Purpose

    genomic Sindbis virus plasmid for expression of SYNseq components

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Inserts

    myc-3xFlag-CLIP-Nrx1B-1xλN(i)
    eGFP
    

    Use

    Synthetic Biology; Sindbis virus genomic plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AB.pCCL.sin.cPPT.GFP.miR-185-5p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85930

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-185-5p Sensor (MIR185 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAG260

    Plasmid

    #33210

    Depositor

    David Bartel

    Article

    Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

    Insert

    SYNM (SYNM Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early enhancer/promoter

    Available since

    Jan. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pgRNA-guideless

    Plasmid

    #129464

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Guideless gRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 907H1

    Plasmid

    #160234

    Purpose

    FlightN-CymR-VP16-3X-SV40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    p-cymene repressor
    Flightin promoter
    

    Use

    Tags

    VP16

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pASKt15C+SrtA5

    Plasmid

    #64985

    Purpose

    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-DUET-1-mRhubarb719-HO

    Plasmid

    #141200

    Purpose

    Expresses mRhubarb719 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb719
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCLR2070

    Plasmid

    #28063

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains N496D point mutation in FokI domain, as …

    Promoter

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCLR2068

    Plasmid

    #28062

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains H537R point mutation in FokI domain, as …

    Promoter

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Dendra2-Keratin-17

    Plasmid

    #57726

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

40. pDS272

    Plasmid

    #34975

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2-GFP-LOVpep (MID2 Budding Yeast, A. sativa)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge