We narrowed to 40,364 results for: vit;

1. ABE8.20-d

   Plasmid

   #136301

   Purpose

   expresses ABE8.20-d in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.20-d
   

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1P CISD1_1

   Plasmid

   #160773

   Purpose

   Suppress CISD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCISD1_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE-HA-GOLPH3

   Plasmid

   #21687

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Golgi Phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. VN-Sph1

   Plasmid

   #89755

   Purpose

   expresses human Synphilin fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Dominguez-Meijide et al Neurobiol Dis. 2021 Apr;151:105256. doi:

   Insert

   SNCAIP (SNCAIP Human)
   

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MRGPRX1-DuET

   Plasmid

   #213344

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   MRGPRX1 (MRGPRX1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-FLAG-APEX2-LGALS3

   Plasmid

   #175756

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1P CISD1_2

   Plasmid

   #160774

   Purpose

   Suppress CISD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCISD1_2
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST_FLAG-HUWE1_delta169-189

   Plasmid

   #187136

   Purpose

   mammalian cell expression of FLAG-HUWE1 delta169-189

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   delta169-189

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5 bovine GRK5

   Plasmid

   #14690

   Depositor

   Robert Lefkowitz

   Article

   Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

   Insert

   GRK5 (GRK5 Bovine)
   

   Expression

   Mammalian

   Available Since

   June 19, 2007

   Availability

   Academic Institutions and Nonprofits only

10. TNIK gRNA (BRDN0001146045)

    Plasmid

    #75849

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TNIK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TNIK (TNIK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-FLEX-PKIalpha-IRES-nls-mRuby2

    Plasmid

    #63059

    Purpose

    Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    PKIalpha (Pkia Synthetic, Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry

    Plasmid

    #232244

    Purpose

    Mammalian expression of N581K RLIM-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    N581K RLIM-GFP-IRES-mCherry (RLIM Human)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    N581K

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS puro GFP-REX1[degron]-IRES-mCherry

    Plasmid

    #232240

    Purpose

    Mammalian expression of REX1[degron]-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    REX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    REX1 (aa 70-85)

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pINDUCER13 (miR-LUP)

    Plasmid

    #46936

    Purpose

    Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest

    Depositor

    Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. LLP469 pEF1a-mCherry-EMPTY-gRNA

    Plasmid

    #100958

    Purpose

    mCherry driven by EF1a, empty gRNA scaffold driven by U6

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    gRNA empty backbone with RFP
    

    Use

    CRISPR

    Tags

    RFP

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

    Plasmid

    #100846

    Purpose

    AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRCaMP1a
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRN3P-HA-Suv4-20h2mut

    Plasmid

    #86692

    Purpose

    For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5C mutant (Kmt5c Mouse)
    

    Tags

    HA

    Mutation

    Mutated asparagine 273 to cysteine 276 (NHDC) int…

    Promoter

    T3

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Flag-hNF2

    Plasmid

    #107150

    Purpose

    mammalian expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

    Insert

    neurofibromin 2 (NF2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-fDIO-GCaMP6f

    Plasmid

    #128315

    Purpose

    Expresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Has Service

    AAV1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    His Tag

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Puro_siKO

    Plasmid

    #86696

    Purpose

    Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    H1 TO

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-FLEX-ArchT-GFP

    Plasmid

    #28307

    Purpose

    Cre-dependent viral expression of ArchT-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGL4.23 WT 2014 EBOV + Mucin-Like Domain

    Plasmid

    #85794

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLV-SspB-HaloTag-TIAM1-DHPH

    Plasmid

    #176127

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMIA3-Pansio

    Plasmid

    #214445

    Purpose

    Expresses eSpCas9 and gRNA targeting Pansio-1 GSH

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    eSpCas9-P2A-mRuby2-P2A-tp53dn
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEM-Cas9HF1-indRecA56

    Plasmid

    #102294

    Purpose

    Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Inserts

    Cas9HF1
    recA56
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pMIA10.5-Clover

    Plasmid

    #214449

    Purpose

    BxbI landing-pad mClover donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    mClover
    

    Expression

    Mammalian

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-Syn-SomArchon-P2A-CoChR-Kv2.1

    Plasmid

    #126945

    Purpose

    Expression of SomArchon-P2A-CoChR-Kv2.1 under Syn promoter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon-P2A-CoChR-Kv2.1
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pGW-Dronpa-Cav1.2

    Plasmid

    #182333

    Purpose

    L-type voltage-gated calcium channel α1C subunit tagged with Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Cav1.2-Dronpa (CACNA1C Rabbit)
    

    Tags

    Full-length rabbit Cav1.2.

    Expression

    Mammalian

    Mutation

    T1066Y, Q1070M

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Halo-Tev-Flag-Ube2v2

    Plasmid

    #110070

    Purpose

    Express Halo-Tev-Flag-Ube2v2 (human) in mammalian cells

    Depositor

    Yimon Aye

    Article

    Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

    Insert

    Halo-Tev-Flag-Ube2v2 (UBE2V2 Human)
    

    Tags

    Halo-TEV-Flag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. MLF2-GFP-3xFLAG

    Plasmid

    #230986

    Purpose

    Expresses GFP tagged MLF2 in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

    Insert

    MLF2 (MLF2 Human)
    

    Tags

    3xFLAG and GFP

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-TRE-DIO-GCaMP6s

    Plasmid

    #183809

    Purpose

    his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene TetO promoter-DIO/flex-GCaMP6
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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32. pCAG/ATP11C(E184Q)-HA

    Plasmid

    #209251

    Purpose

    Mammalian expression of ATP11C mutant E184Q

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP11C (ATP11C Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E184Q

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG/ATP8A1(E191Q)-HA

    Plasmid

    #209240

    Purpose

    Mammalian expression of ATP8A1 mutant E191Q

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8A1 (ATP8A1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E191Q

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA 3.3 hFZD4

    Plasmid

    #115908

    Purpose

    Expresses human FZD4 in mammalian cells.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    FZD4 (FZD4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPRKCZ-C1-EGFP (C-PRKCZ-C1)

    Plasmid

    #217759

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C1 domain (aa 123-193)

    Promoter

    CMV

    Available Since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pCW57.1-Neurod1-3xT7

    Plasmid

    #200890

    Purpose

    Inducible lentiviral expression of Neurod1

    Depositor

    Kai Ge

    Article

    Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

    Insert

    Neurod1 (Neurod1 Mouse)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    3xT7

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMIA3-Keppel

    Plasmid

    #214446

    Purpose

    Expresses eSpCas9 and gRNA targeting Keppel-19 GSH

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    eSpCas9-P2A-mRuby2-P2A-tp53dn
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 409 pSG5L HA RB (379-928)

    Plasmid

    #10734

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB (379-928) (RB1 Human)
    

    Expression

    Mammalian

    Mutation

    amino acids 379-928

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

39. pAC150-RFP-EGFP-LGALS3 R186S

    Plasmid

    #175779

    Purpose

    PiggyBac Vector expressing GFP-RFP-LGALS3 R186S

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Expression

    Mammalian

    Mutation

    R186S

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Flag WDR24 pRK5

    Plasmid

    #46334

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR24 (WDR24 Human)
    

    Tags

    FLag

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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