We narrowed to 26,493 results for: vit;

1. non-targeting control gRNA (BRDN0001149469)

   Plasmid

   #80257

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST_FLAG-HUWE1_BH3-4E

   Plasmid

   #187144

   Purpose

   mammalian cell expression of FLAG-HUWE1 V1976E/V1780E/L1983E/M1987E

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   V1976E/V1780E/L1983E/M1987E

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST_FLAG-HUWE1_delta3291-3298

   Plasmid

   #187125

   Purpose

   mammalian cell expression of FLAG-HUWE1 delta3291-3298

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   delta3291-3298

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ABE8.8-d

   Plasmid

   #136295

   Purpose

   expresses ABE8.8-d in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.8-d
   

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

   Plasmid

   #181814

   Purpose

   Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   Muc5b D1 (Muc5b Mouse)
   

   Tags

   TEV cleavage site followed by 6xHistidine tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-gfap-sgRNA

   Plasmid

   #65566

   Purpose

   in vitro trancription of sgRNA targeting the zebrafish gfap locus

   Depositor

   Jiu-lin Du

   Article

   Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

   Insert

   gfap sgRNA (gfap Zebrafish)
   

   Use

   In vitro rna transcription

   Promoter

   T7

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKT2/CAGXSP/PalmNanoLuc

   Plasmid

   #182969

   Purpose

   Stably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.

   Depositor

   Masamitsu Kanada

   Article

   Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

   Insert

   PalmNanoLuc
   

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEBG-IFNAR2(265-515)

   Plasmid

   #12437

   Depositor

   Dong-Er Zhang

   Article

   Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

   Insert

   IFNAR2 a.a. 265-515 (IFNAR2 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   pEBG vector is from Bruce Mayer, may be different…

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pLKO.1P DDX11_2

   Plasmid

   #160778

   Purpose

   Suppress DDX11

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shDDX11_2
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1P DDX11_1

    Plasmid

    #160777

    Purpose

    Suppress DDX11

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shDDX11_1
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-A3A-seBE4max-IRES

    Plasmid

    #174698

    Purpose

    Small-molecule controlled base editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    A3A-seBE4max-IRES (APOBEC3A Human)
    

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    A3A-M13I, UGI-E11K

    Promoter

    CMV

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR4 PARP2 E614Q

    Plasmid

    #192545

    Purpose

    pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)
    

    Use

    Gateway entry vector

    Mutation

    changed Glu 614 to Gln

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-mCherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176138

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAT15546_nABE8e-SuperFi-Cas9

    Plasmid

    #184376

    Purpose

    Mammalian expression of SuperFi-Cas9 ABE8e base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    nABE8e-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1P POLE2

    Plasmid

    #160766

    Purpose

    Suppress POLE2

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Sumo3 PolQM1 deltains1

    Plasmid

    #78461

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie, Susan Wallace

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; delta 2149-2170; Q2513R (please see…

    Promoter

    T7

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

17. non-targeting control gRNA (BRDN0001147780)

    Plasmid

    #80226

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. WEE1 gRNA (BRDN0001149349)

    Plasmid

    #77367

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human WEE1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    WEE1 (WEE1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKS7107

    Plasmid

    #89051

    Purpose

    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Nm crRNA
    Nm tracrRNA
    hNmCas9
    

    Use

    CRISPR

    Tags

    HA tag, NLS, and SV40 NLS

    Promoter

    EF1a and human U6

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PIM1 gRNA (BRDN0001146465)

    Plasmid

    #77174

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIM1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIM1 (PIM1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PIM1 gRNA (BRDN0001146597)

    Plasmid

    #77175

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIM1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIM1 (PIM1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PIM1 gRNA (BRDN0001145078)

    Plasmid

    #77176

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIM1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIM1 (PIM1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTE4560

    Plasmid

    #107526

    Purpose

    Expresses As crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    As crRNA
    mCherry
    

    Expression

    Mammalian

    Promoter

    CBh and human U6

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. WT N-His Ana GvpC in pET28a

    Plasmid

    #153294

    Purpose

    Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (N-terminal his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added a hexahistidine tag after the methionine at…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-FLEX-tmeGFP-IRES-nls-mRuby2

    Plasmid

    #63060

    Purpose

    Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    nls-mRuby2
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p260 pCMV-CRE-M-AR(wt)

    Plasmid

    #12494

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Insert

    CRE-M AR (AR Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Mutation

    CRE-M and AR ligand binding domain

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pGL3-MTS-CCDB-N136-RsDddA-A-UGI-PURO

    Plasmid

    #205463

    Purpose

    A backbone containing a RsDddA fragment A for constructing mitochondria-targeted RsDdCBE expression plasmid

    Depositor

    Bin Shen

    Article

    Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

    Insert

    MTS; N136; ccdb; RsDddA-A; UGI
    

    Expression

    Mammalian

    Available Since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL3-MTS-CCDB-N136-RsDddA-C-UGI-PURO

    Plasmid

    #205465

    Purpose

    A backbone containing a RsDddA fragment C for constructing mitochondria-targeted RsDdCBE expression plasmid

    Depositor

    Bin Shen

    Article

    Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

    Insert

    MTS; N136; ccdb; RsDddA-C; UGI
    

    Expression

    Mammalian

    Available Since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pF714-pET28a-Hs-FL-METTL20-NHis wt

    Plasmid

    #85115

    Purpose

    expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length METTL20 (METTL20 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

30. WEE1 gRNA (BRDN0001145570)

    Plasmid

    #77368

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human WEE1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    WEE1 (WEE1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pF711-pET28a-Hs-delta25-MCAD-NHis wt

    Plasmid

    #85112

    Purpose

    expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta25) MCAD (ACADM Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ROCK1 gRNA (BRDN0001147709)

    Plasmid

    #77593

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROCK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROCK1 (ROCK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PGEX-6P1-hNF2-AR mutant

    Plasmid

    #107149

    Purpose

    bacterial expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

    Insert

    neurofibromin 2 (NF2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    A585W-R588K

    Promoter

    Ptac

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag

    Plasmid

    #187994

    Purpose

    Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cells

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    mucin2 D1D2D'D3 (MUC2 Human)
    

    Tags

    6xHistadine followed by TEV cleavage site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. non-targeting control gRNA (BRDN0001145869)

    Plasmid

    #80256

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDONR221_SLC39A14

    Plasmid

    #132105

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A14 (SLC39A14 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pTRE Tight ArchT-GFP

    Plasmid

    #79627

    Purpose

    Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

    Depositor

    Yasunori Hayashi

    Article

    Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

    Insert

    ArchT
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ArchT is a more sensitive version of Arch (archae…

    Promoter

    Tet responsive Ptight promoter

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

38. pLKO.1P POLD1_1

    Plasmid

    #160791

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_1
    

    Use

    Lentiviral

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLKO.1P REV3L_2

    Plasmid

    #160798

    Purpose

    Suppress REV3L

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shREV3L_2
    

    Use

    Lentiviral

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLKO.1P REV3L_1

    Plasmid

    #160797

    Purpose

    Suppress REV3L

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shREV3L_1
    

    Use

    Lentiviral

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge