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pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR

Plasmid

#126679

Purpose

Fluorescent reporter for cyclin E/A-CDK and CDK4/6 activities

Depositor

Hee Won Yang

Article

Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

Insert

Rb (a.a 886-928)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pOVC1_RsLP-LOV_X

Plasmid

#131177

Purpose

Expresses RsLP-LOV (Rhodobacter sphaeroides) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

RsLP-LOV

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Plasmid

#177919

Purpose

Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p0521s

Plasmid

#62862

Purpose

Episome vector for Phaeodactylum tricornutum (pCC1BAC-based)

Depositor

Hamilton Smith

Article

Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Episomal vector for phaeodacty…

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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KAP3-FL

Plasmid

#51413

Purpose

expression of EGFP-tagged full length human KAP3B in mammalian cells

Depositor

Trina Schroer

Article

Brown et al Traffic. 2005 Dec;6(12):1114-24.

Insert

Kinesin-associated protein 3 (KIFAP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-KDM4D

Plasmid

#188234

Purpose

Express human KDM4D in mammalian cells

Depositor

Young Tang

Article

Su et al Int J Mol Sci. 2021 Sep 28;22(19). pii:

Insert

Lysine demethylase 4D (KDM4D Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA MOV10 K530A

Plasmid

#51716

Purpose

plasmid expresses FLAG/HA-tagged MOV10 K530A

Depositor

Markus Landthaler

Article

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 K530A (MOV10 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

K530A

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA MOV10 D645N

Plasmid

#51717

Purpose

plasmid expresses FLAG/HA-tagged MOV10 D645N

Depositor

Markus Landthaler

Article

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 D645N (MOV10 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

D645N

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-I3-SYFP2

Plasmid

#189763

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCGT001_nuclear-mOrange2

Plasmid

#248083

Purpose

Constitutive expression of NLS-tagged mOrange2 from EF1-alpha promoter (for lentiviral packaging)

Depositor

Arjun Raj

Article

Van Eyndhoven et al bioRxiv [Preprint]. 2025 Jun 27:2025.06.

Insert

mOrange2

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCRU5-inGFPt

Plasmid

#60236

Purpose

HTLV-1 based transfer vector encoding gfp-turbo reporter gene interrupted by intron

Depositor

Dmitriy Mazurov

Article

Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

Insert

inGFPt

Use

Retroviral

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pTorPE-B-GECO1

Plasmid

#32469

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

B-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-eGFP

Plasmid

#193022

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the cytomegalovirus (CMV) promoter

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2023 Jan 13. doi: 10.1038/s41

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP1354

Plasmid

#119275

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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aCKAR

Plasmid

#124427

Purpose

FRET-based reporter for monitoring aPKC activity in cells

Depositor

Alexandra Newton

Article

Kajimoto et al Sci Signal. 2019 Jan 1;12(562). pii: 12/

Insert

aCKAR

Tags

mCFP and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET-WHaloCaMP1b-EGFP

Plasmid

#205305

Purpose

Expression of WHaloCaMP1b-EGFP fusion in E. coli

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1b-EGFP

Expression

Bacterial

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-mScarlet

Plasmid

#207960

Purpose

Vector for overexpression of the mScarlet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mScarlet

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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dsRed-cent2

Plasmid

#29523

Depositor

Joseph Gleeson

Article

Tanaka et al J Cell Biol. 2004 Jun 7. 165(5):709-21.

Insert

Cent2 (CETN2 Human)

Tags

dsRed

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pAF0688 Kp03_attP-mCherry donor

Plasmid

#193467

Purpose

Promoterless mcherry donor for Kp03

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03_attP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-G-GECO1.1

Plasmid

#32445

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Expression

Mammalian

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Keap1

Plasmid

#62454

Purpose

His6 tagged Keap1 for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Tag

Expression

Bacterial

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pISCba-reRNA

Plasmid

#226823

Purpose

Expressing ISCba controled by PlacM promoter, guide RNA controled byPJ23119 promoter

Depositor

Sheng Yang

Article

Wang et al ACS Synth Biol. 2025 May 8. doi: 10.1021

Insert

ISCba and its corresponding guide RNA

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pISClsp3-reRNA

Plasmid

#226824

Purpose

Expressing ISClsp3 controled by PlacM promoter, guide RNA controled by PJ23119 promoter

Depositor

Sheng Yang

Article

Wang et al ACS Synth Biol. 2025 May 8. doi: 10.1021

Insert

ISClsp3 and its corresponding guide RNA

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-BICDN-CIBN

Plasmid

#102254

Purpose

Expresses fusion of dynein adapter BICDN (1-594) with GFP and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

GFP-BICDN-CIB1 (CIB1 Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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AAV sgRNA Expression Plasmid

Plasmid

#174540

Purpose

Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

Depositor

Ormond MacDougald

Article

Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

Type

Empty backbone

Use

AAV

Tags

None

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pJAB988

Plasmid

#133920

Purpose

miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter

Depositor

Christopher Voigt

Article

Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.

Insert

miniICE-tet(M)cat(PC194)_PT7-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAF0451 Si74_attP-mCherry donor

Plasmid

#193470

Purpose

Promoterless mcherry donor for Si74

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Si74_attP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDAC569

Plasmid

#195241

Purpose

Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP

Expression

Mammalian

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-G-GECO1.0

Plasmid

#32447

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.0

Expression

Mammalian

Mutation

GCaMP3 K119I/L173Q/S404G/E430V

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pGRACE

Plasmid

#230910

Purpose

Tracing of CRISPR activity in whole animals.

Depositor

Michael Boutros

Article

Port et al Nat Commun. 2026 Jan 15;17(1):877. doi:

Insert

GFP

Use

CRISPR

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-Akt1(WT)

Plasmid

#86631

Purpose

Expresses mCherry-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-CoV2-WT

Plasmid

#177618

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-mfap4

Plasmid

#70052

Purpose

5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

mfap4 promoter

Use

Gateway 5' element

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ExRai-AktAR

Plasmid

#118462

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AktAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221_HUWE1_isoform1

Plasmid

#190464

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_SynP_DIO_tAKARα- T391A_WPRE

Plasmid

#196676

Purpose

AAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).

Depositor

Haining Zhong

Article

Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

Insert

tAKARα-T391A

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry-Rab11a (Q70L)

Plasmid

#184037

Purpose

Expresses constitutively active mCherry Rab11a in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

Insert

Rab11

Tags

mCherry

Expression

Mammalian

Mutation

Glutamine 70 changed to Leucine

Promoter

CMV IE94

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FH-TET3-HxDmut-pEF

Plasmid

#127896

Purpose

Expresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079A

Depositor

Patrick Hogan, Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

TET3-HxDmut (TET3 Human)

Tags

HA and Flag

Expression

Mammalian

Promoter

EF-1a

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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3xFLAG-SENP7

Plasmid

#42886

Depositor

Edward Yeh

Article

Bawa-Khalfe et al Proc Natl Acad Sci U S A. 2012 Oct 23;10

Insert

SENP7 (SENP7 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

T102A, R969C, L996S

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-Arp11 (1-417)

Plasmid

#51398

Purpose

expression of full-length mouse Arp11 in mammalian cells

Depositor

Trina Schroer

Article

Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

Insert

Arp11 (Actr10 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2014

Availability

Academic Institutions and Nonprofits only
HA-Daxx/pRK5

Plasmid

#52023

Purpose

Expresses HA-Daxx in mammalian cells

Depositor

Xiaolu Yang

Article

Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

Insert

HA-Daxx (DAXX Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT449

Plasmid

#139389

Purpose

Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)

Use

Synthetic Biology

Promoter

Bile Acid inducible Promoter

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SOX D221A/E244A/K246A pACEmam1

Plasmid

#192050

Purpose

Expresses a 3X catalytic mutant of SOX (ORF37)

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (3X catalytic mutant)

Use

Unspecified

Mutation

3X catalytic mutant D221A/E244A/K246A

Promoter

CMV

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Rubicon

Plasmid

#221659

Purpose

Rubicon tagged N-terminally with EGFP; clone a gift from the Hurley Lab at UC Berkeley

Depositor

Erika Holzbaur

Article

Basak et al Nat Commun. 2025 Aug 9;16(1):7367. doi:

Insert

RUBCN (RUBCN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIBA3-SpyCatcher-sfGFP

Plasmid

#107420

Purpose

For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

Depositor

Jack Leo

Article

Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

Insert

SpyCatcher

Tags

His tag, StrepII, and superfolder GFP

Expression

Bacterial

Promoter

Tet

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP1055

Plasmid

#119242

Purpose

construction intermediate

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Available Since

Dec. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
UAST-uMCas12a+nls2x

Plasmid

#230909

Purpose

inducible expression of Cas12a+ with Gal4.

Depositor

Michael Boutros

Article

Port et al Nat Commun. 2026 Jan 15;17(1):877. doi:

Insert

Cas12a+

Use

CRISPR

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pZac2.1-GfaABC1D-GAT3-HA-mCherry

Plasmid

#117722

Purpose

Expresses a GABA transporter GAT-3 (Slc6a11)

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Slc6a11 (Slc6a11 Mouse)

Use

AAV

Tags

HA and mCherry

Expression

Mammalian

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mas-GRK2RH-Nluc

Plasmid

#158605

Purpose

Plasmid expressing GRK2(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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