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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 84,971 results for: TRI

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Showing: 11151 - 11200 of 84971 results

  1. pET28a(+)::MBP-NL3F10H

    Plasmid
    #141291
    Purpose
    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    MBP-NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3), His (x10), and MBP
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available Since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    141291-plasmid-map-sequence-id-324616

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  8. pET28a-HsSENP1

    Plasmid
    #71465
    Purpose
    Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 1 (SENP1 Human)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. pSNRW-Z5-Nluc

    Plasmid
    #174487
    Purpose
    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.
    Depositor
    Richard C. Willson
    Article
    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1
    Insert
    Z5-Nluc
    Tags
    6x Histidine tag (N terminal on insert)
    Expression
    Bacterial
    Promoter
    T7lac promoter
    Available Since
    Oct. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174487-plasmid-map-sequence-id-345291

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  32. pCJR01

    Plasmid
    #187888
    Purpose
    Escherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.
    Depositor
    Gaylen Uhlich
    Article
    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144
    Insert
    DNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187888-plasmid-map-sequence-id-368412

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  47. PML-3/pSG5

    Plasmid
    #50939
    Purpose
    expression of PML with Sumo sites destroyed
    Depositor
    Pier Pandolfi
    Article
    Zhong et al Blood. 2000 May 1;95(9):2748-52.
    Insert
    PML-3 (PML Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    K65R, K160R, K490R (Sumo modification sites)
    Promoter
    SV40
    Available Since
    Jan. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 11151 - 11200 of 84971 results