We narrowed to 11,615 results for: VARS

1. CoV2-N-A119S

   Plasmid

   #177948

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (A119S). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (A119S) (N )
   

   Expression

   Mammalian

   Mutation

   A119S

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-CMV-jGCaMP8f F366H

   Plasmid

   #204141

   Purpose

   Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f F366H
   

   Tags

   N-terminal His tag

   Expression

   Mammalian

   Mutation

   Phenylalanine 366 changed to histidine

   Promoter

   CMV

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

   Plasmid

   #192683

   Purpose

   Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p1.1-eGFP

   Plasmid

   #162738

   Purpose

   Fluorescent reporter for CHO cells expression studies

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Insert

   enhanced green fluorescent protein
   

   Expression

   Mammalian

   Mutation

   consensus Kozak sequence (GCCGCCATGG) added befor…

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Syn-ChromeQ-GFP

   Plasmid

   #123317

   Purpose

   AAV-mediated expression of ChromeQ-GFP under the Syn promoter.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeQ-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

   Promoter

   Syn

   Available Since

   March 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV iGluf

   Plasmid

   #105833

   Purpose

   Fast indicator for extracellular glutamate imaging

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR E25D variant
   

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Changed Glu 25 to Asp

   Promoter

   CMV

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. KIF5C(1-560)-DmrC

   Plasmid

   #61673

   Purpose

   Non-fluorescent, truncated kinesin-1 fused to DmrC linker

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KIF5C(1-560)-DmrC (Kif5c Rat)
   

   Expression

   Mammalian

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_PIK3R2-1/1

   Plasmid

   #91415

   Purpose

   Protein expression and purification of human SH3 domain construct PIK3R2-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   PIK3R2-1/1 (PIK3R2 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTA231-p.Syn-Dendra2Hfx

   Plasmid

   #164660

   Purpose

   expression of H. volcanii codon-optimized Dendra2

   Depositor

   Anita Marchfelder

   Article

   Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010.

   Insert

   Dendra2Hfx
   

   Use

   Archaeal expression

   Mutation

   codon-optimization for Haloferax volcanii

   Promoter

   p.Syn

   Available Since

   Feb. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAd GFP HDAC5 S259A/S498A

    Plasmid

    #32218

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Adenoviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S259A/S498A

    Available Since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

11. sg1+sg2+sg3

    Plasmid

    #113969

    Purpose

    Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg1+sg2+sg3
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD

    Plasmid

    #168135

    Purpose

    Inducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. CoV2-N-M234I

    Plasmid

    #177955

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (M234I). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (M234I) (N )
    

    Expression

    Mammalian

    Mutation

    M234I

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-rtTA-zeo

    Plasmid

    #183233

    Purpose

    Lentiviral vector expressing the rtTa gene

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    rtTA-T2A-Zeo
    

    Use

    Lentiviral

    Promoter

    UBC

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_NOXO1-1/2

    Plasmid

    #91499

    Purpose

    Protein expression and purification of human SH3 domain construct NOXO1-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NOXO1-1/2 (NOXO1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103_SHANK3-1/1

    Plasmid

    #91421

    Purpose

    Protein expression and purification of human SH3 domain construct SHANK3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SHANK3-1/1 (SHANK3 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TTR C10S S85C K80D

    Plasmid

    #184743

    Purpose

    bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

    Insert

    TTR (TTR Human)
    

    Expression

    Bacterial

    Mutation

    C10S, S85C, K80D

    Promoter

    T7

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNHT7::RPL15::hGluc

    Plasmid

    #186768

    Purpose

    An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Gaussia luciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_TNK1-1/1-SV1/3

    Plasmid

    #91548

    Purpose

    Protein expression and purification of human SH3 domain construct TNK1-1/1-SV1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TNK1-1/1-SV1/3 (TNK1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_SH3GL3-1/1

    Plasmid

    #91423

    Purpose

    Protein expression and purification of human SH3 domain construct SH3GL3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3GL3-1/1 (SH3GL3 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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