We narrowed to 15,977 results for: LEA
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Plasmid#80937PurposeExpresses Cas9N in mammalian cells; expresses gRNA M4 for Mstn cleavage.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-FERM10
Plasmid#59126PurposeFAK fret control sensor without kinase domain with 10 nm linkerDepositorInsertFocal Adhesion Kinase
TagsFLAG, mCerulean, and mCitrineExpressionMammalianPromoterCMVAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
mPapaya1-Nup50-N-10
Plasmid#56355PurposeLocalization: Nuclear Pore, Excitation: 530, Emission: 541DepositorAvailable SinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1756
Plasmid#53036PurposePconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialPromoterpConNoHind and pConNoHindM12Available SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEG585
Plasmid#40192DepositorInsertMex-5 (S404A) (RNAi resistant) (mex-5 Nematode)
TagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationS404A; exon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable SinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
L4872 VP64-intN in PolyTX-mNeonGreen
Plasmid#244191PurposeConstitutive expression of VP64-intN split synTF componentDepositorInsertSplit synTF component VP64-intN
UseSynthetic BiologyTags3xFLAG - nuclear localization sequenceExpressionMammalianPromoterhEF1aAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
RC00214-pcsb-35Sp-ccdB-mNG-OCSp-tdT
Plasmid#209019PurposeDual-fluorescence acceptor plasmid for one-step insertion of a 5' leader of interest between the CaMV35S promoter and mNeonGreen via BpiI. A tdTomato expression cassette serves as internal control.DepositorInsertsccdB
mNeonGreen
tdTomato
ExpressionPlantMutationcodon optimized for N. benthamianaPromoterCaMV35S and Octopine SynthaseAvailable SinceOct. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET50del_SynPspA_H1-5
Plasmid#223820PurposeBacterial expression of SynPspA H1-5 with N-terminal 6xHis-Tag and 3C cleavage siteDepositorInsertSynPspA
Tags6xHisExpressionBacterialMutationtruncation of helix 0Available SinceSept. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET50delCT_Vipp1
Plasmid#223815PurposeBacterial expression of Vipp1 with C-terminal 6x His-Tag and 3C cleavage siteDepositorInsertVipp1
Tags6xHisExpressionBacterialAvailable SinceSept. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET50delCT_Vipp1_H1-6
Plasmid#223816PurposeBacterial expression of Vipp1 H1-6 with C-terminal 6x His-Tag and 3C cleavage siteDepositorInsertVipp1
Tags6xHisExpressionBacterialMutationtruncation of helix 0Available SinceSept. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACE2-Mutant-R652A
Plasmid#240605PurposeTo test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2; R was substituted by A at Residue 652 of ACE2 in the collectrin-like siteDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFLAG tagExpressionMammalianMutationArginine (R) at position 652 on ACE2 is replaced …PromoterCMVAvailable SinceSept. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET50del_SynPspA
Plasmid#223818PurposeBacterial expression of SynPspA with N-terminal 6xHis-Tag and 3C cleavage siteDepositorInsertSynPspA
Tags6xHisExpressionBacterialAvailable SinceAug. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACE2-Mutant-C1
Plasmid#240604PurposeTo test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 1 located within the collectrin-like site on ACE2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFLAG tagExpressionMammalianMutationArginine (R) and Lysine (K) residues on ACE2-clus…PromoterCMVAvailable SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACE2-Mutant-DeltaC4
Plasmid#240609PurposeTo test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2 likely at Cluster 4 of ACE2 in the collectrin-like site. The whole C4 sequence was deleted in this mutantDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFLAG tagExpressionMammalianMutationdeletion of the full Cluster 4 sequence on ACE2 (…PromoterCMVAvailable SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACE2-Mutant-C0
Plasmid#240603PurposeTo test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 0 located within the collectrin-like site on ACE2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFLAG tagExpressionMammalianMutationArginine (R) and Lysine (K) residues on ACE2-clus…PromoterCMVAvailable SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACE2-Mutant-C3
Plasmid#240607PurposeTo test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 3 located within the collectrin-like site on ACE2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFLAG tagExpressionMammalianMutationArginine (R) and Lysine (K) residues on ACE2-clus…PromoterCMVAvailable SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pαH-NiVop08-Foldon
Plasmid#200390PurposeMammalian cell expression of Nipah virus fusion protein with prefusion stabilizing mutationsDepositorInsertNiVop08 Ectodomain
TagsFoldon, HRV3C cleavage site, 8X His tag, 2X Strep…ExpressionMammalianMutationEctodomain (AA 1-488), L104C, I114C, L172F, S191PAvailable SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_H2B-HaloTag7_T2A_mEGFP
Plasmid#187070PurposeNuclear expression of HaloTag7 in mammalian cellsDepositorInsertH2B_HaloTag7
ExpressionMammalianAvailable SinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_H2B-dHaloTag7_T2A_mEGFP
Plasmid#187071PurposeNuclear expression of dHaloTag7 in mammalian cellsDepositorInsertH2B_dHaloTag7
ExpressionMammalianMutationdHaloTag7 = HaloTag7-D106AAvailable SinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
IKKε v2-V5
Plasmid#169770PurposeExpress IKKε v2 in mammalian cellsDepositorInsertHomo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2
TagsV5 tagExpressionBacterial and MammalianAvailable SinceJuly 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
F-IKKε v2
Plasmid#169773PurposeExpress IKKε v2 in mammalian cellsDepositorInsertHomo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2
TagsFlag tagExpressionBacterial and MammalianAvailable SinceJuly 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-H2B-iRFP
Plasmid#214830PurposeEncoding nuclear-targeted iRFP713.DepositorInsertiRFP with nucleus-targeting sequence
UseLentiviralTagsH2B (human histone 2B)ExpressionMammalianPromoterEF-1αAvailable SinceJuly 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
CKII-NPTX1-pH
Plasmid#217974PurposeExpression of NPTX1-pHluorin for optical monitoring of presynaptic release in neuronsDepositorInsertNeuronal Pentraxin 1-pHluorin
UseLentiviralTagspHluorinExpressionMammalianPromoterCaMKIIaAvailable SinceJuly 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-U6-CNCB
Plasmid#236763PurposeA piggybac-based cloning vector containing mouse U6 promoter-driven sgRNA for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.DepositorTypeEmpty backboneUsePiggybacExpressionMammalianPromotermouse U6 promoter, CAG promoterAvailable SinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE3G-CCRB
Plasmid#236772PurposeA piggybac-based cloning vector containing TRE3G promoter followed by cloning site and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.DepositorTypeEmpty backboneUsePiggybacExpressionMammalianPromoterTRE3G promoter, CAG promoterAvailable SinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-2G-CNCB
Plasmid#236764PurposeA piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.DepositorTypeEmpty backboneUsePiggybacExpressionMammalianPromotermouse U6 promoter, human U6 promoter, CAG promoterAvailable SinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB164
Plasmid#217842Purpose10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expressionDepositorInsertHaloTag
Tags10x His, HaloTag, and SUMO (SMT3)ExpressionBacterialPromoterT7 PromoterAvailable SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-TEV-AtPCO4-2(wt)
Plasmid#191802PurposeFull length wt AtPCO4-2 with Nt-His tagDepositorInsertAtPCO4-2
Tags6xHis, TEV cleavage siteExpressionBacterialAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_NES_V5_GFP
Plasmid#235632PurposeEncodes V5-GFP with an N-terminal nuclear export sequence. Used as a no-ALIBi negative control.DepositorInsertGFP
TagsNES-V5ExpressionMammalianAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1061 (pLenti6.2_CMV_GATA1_P2A_H2A_mCherry)
Plasmid#233543PurposeA CMV driven human GATA1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.DepositorAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 solvvol
Plasmid#232976PurposeGalactose iduced expression of Gcn4 solvvol in yeastDepositorInsertGcn4 solvvol
TagsTEV cleavage siteExpressionYeastMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoED WHA
Plasmid#232995PurposeGalactose iduced expression of Gcn4 ILVtoED W+HAin yeastDepositorInsertGcn4 ILVtoED W+
Tags3xHA and TEV cleavage siteExpressionYeastMutationN126W, I128E, V130E, V135E, L137D, I142DPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 9acidAHA
Plasmid#232997PurposeGalactose iduced expression of Gcn4 9acidAHA in yeastDepositorInsertGcn4 9acidA
Tags3xHA and TEV cleavage siteExpressionYeastMutationE109A, E111A, E114A, D115A, E119A, D125A, D127A, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 9acidAGFP
Plasmid#233009PurposeGalactose iduced expression of Gcn4 9acidAGFP in yeastDepositorInsertGcn4 9acidA
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationE109A, E111A, E114A, D115A, E119A, D125A, D127A, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoED WGFP
Plasmid#233007PurposeGalactose iduced expression of Gcn4 LVtoED W+GFPin yeastDepositorInsertGcn4 ILVtoED W+
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationN126W, I128E, V130E, V135E, L137D, I142DPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 solvvol W
Plasmid#232977PurposeGalactose iduced expression of Gcn4 solvvol W+ in yeastDepositorInsertGcn4 solvvol W+
Tags3xHA and TEV cleavage siteExpressionYeastMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4003
Plasmid#215248PurposeModule for the constitutive expression of PIF6 and PhyB:VP16DepositorInsertPIF6-PhyB:VP16
UseSynthetic BiologyMutationV234L in PIF6- please see dep. sequence, BsaI and…Available SinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 SATtoG KtoR
Plasmid#231861PurposeBacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoRDepositorInsertGcn4 SATtoG KtoR
Tags6xHis, TEV cleavage siteExpressionBacterialMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterT7Available SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1394 (pME-ZIC3-P2A-H2A-mCherry)
Plasmid#232133PurposeA middle entry gateway clone containing V5 tagged human ZIC3 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1063 (pLenti6.2_CMV_V5_MAFB_P2A_H2A_mCherry)
Plasmid#233545PurposeA CMV driven human MAFB followed by a P2A cleavage peptide and an H2A mCherry fusion protein.DepositorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1473 (pLenti-BLAST-ZIC3-P2A-H2A-mCherry)
Plasmid#233547PurposeA CMV driven human ZIC3 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.DepositorInsertZIC3 (ZIC3 Human)
UseLentiviralAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Sga-Cas9
Plasmid#227005PurposeMammalian expression of Nuclease active S. gallolyticus Cas9DepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLSExpressionMammalianPromoterCMVAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Sin-Cas9
Plasmid#227006PurposeMammalian expression of Nuclease active S. iniae Cas9DepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLSExpressionMammalianPromoterCMVAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Spa-Cas9
Plasmid#227007PurposeMammalian expression of Nuclease active S. parasanguinis Cas9DepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLSExpressionMammalianPromoterCMVAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Sub-Cas9
Plasmid#227008PurposeMammalian expression of Nuclease active S. uberis Cas9DepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLSExpressionMammalianPromoterCMVAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His_TEV_MsbA
Plasmid#191476PurposeExpresses MsbA with a TEV protease cleavable N terminal His TagDepositorInsertMsbA
TagsHisExpressionBacterialPromoterT7Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-Bcx
Plasmid#191195PurposeFusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & Bcx fusion
Tags6xHis and Bacillus circulans xylanaseExpressionBacterialPromoterT7 promoterAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
L469R USP7 catalytic
Plasmid#227212PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationL469R; aa 207-560PromoterT7-promoterAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET_SynPspA
Plasmid#223812PurposeBacterial expression of SynPspA with N-terminal 10xHis-tag and EK cleavage siteDepositorInsertSynPspA
Tags10xHis and NusAExpressionBacterialAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-PKP3(305-797)
Plasmid#202599PurposeExpresses ARM domain of plakophilin3DepositorInsertplakophilin3 (PKP3 Human)
TagsHis8 tag with precision protease cleavage siteExpressionBacterialMutationDeleted amino acids 1 to 304Available SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only