We narrowed to 15,977 results for: LEA

1. pET28a-OuIAD-AE

   Plasmid

   #226968

   Purpose

   Expressing Olsenella uli indoleacetate decarboxylase activating enzyme (IAD-AE) in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   OuIAD-AE
   

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-OuIAD

   Plasmid

   #226967

   Purpose

   Expressing Olsenella uli indoleacetate decarboxylase (IAD) in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   OuIAD
   

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET24a_SMOX

   Plasmid

   #186345

   Purpose

   C-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cells

   Depositor

   Sandra Gabelli

   Article

   Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

   Insert

   Spermine oxidase (SMOX Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. L299A USP7 catalytic

   Plasmid

   #227213

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   L299A; aa 207-560

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. V485G USP7 catalytic

   Plasmid

   #227209

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   V485G; aa 207-560

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. P273H USP7 catalytic

   Plasmid

   #227208

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   P273H; aa 207-560

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Y331H USP7 catalytic

   Plasmid

   #227205

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Y331H; aa 207-560

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. M225I USP7 catalytic

   Plasmid

   #227206

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   M225I; aa 207-560

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. USP7 N418S catalytic

   Plasmid

   #227202

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   N418Sl; aa 207-560

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. K420E USP7 catalytic

    Plasmid

    #227210

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    K420E; aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. USP7 G392D catalytic

    Plasmid

    #227203

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G392D; aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. E345K USP7 catalytic

    Plasmid

    #227211

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    E345K; aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. L373F USP7 catalytic

    Plasmid

    #227207

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L373F; aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. G392A USP7 catalytic

    Plasmid

    #227204

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G392A; aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET50delCT_Vipp1_dL10Ala

    Plasmid

    #223817

    Purpose

    Bacterial expression of Vipp1_dL10Ala with C-terminal 6x His-Tag and 3C cleavage site

    Depositor

    Carsten Sachse

    Article

    Junglas et al Nat Struct Mol Biol. 2025 Mar;32(3):555-

    Insert

    Vipp1
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    replacement of 10 aa in Hinge 2 with Ala

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a:ChrH

    Plasmid

    #225084

    Purpose

    Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrH
    

    Tags

    His6-Thrombin

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVUT-spGFP

    Plasmid

    #225486

    Purpose

    Tet-on lentiviral vector for controlled expression of secretable and permeable EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Igκ leader and TAT peptide
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Human ubiquitin C

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST17-ScChd1[Y137A]

    Plasmid

    #221198

    Purpose

    ScChd1-aa118-1274 chromatin remodeler with Y137A mutation

    Depositor

    Gregory Bowman

    Article

    Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

    Insert

    ScChd1 (aa118-1274)
    

    Tags

    6xHis-tag (Precission protease cleavable)

    Expression

    Bacterial

    Mutation

    Y137A

    Promoter

    T7

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MARS-nGFP

    Plasmid

    #205234

    Purpose

    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
    

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3-2xMARS-PLD

    Plasmid

    #205237

    Purpose

    Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF

    Depositor

    Jeremy Baskin

    Inserts

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    phospholipase D (PLD)
    

    Tags

    Nuclear Export Sequence and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-Hyg-nCas9n

    Plasmid

    #217487

    Purpose

    Expression of nuclear nCas9n

    Depositor

    Bertrand Collet

    Article

    Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21b-hHNF4aORF-1

    Plasmid

    #206959

    Purpose

    The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.

    Depositor

    Catharine Ross, Reza Zolfaghari

    Insert

    HNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFastBac1-bullfrog saxiphilin

    Plasmid

    #140595

    Purpose

    protein expression

    Depositor

    Dan Minor

    Article

    Yen et al Sci Adv. 2019 Jun 19;5(6):eaax2650. doi:

    Insert

    saxiphilin bullfrog
    

    Tags

    GFP-His10

    Expression

    Insect

    Mutation

    Deletion of M20 - please see depositor comment

    Promoter

    polyhedrin promoter

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-NLS-MBP-rATOM(WDR5)

    Plasmid

    #209710

    Purpose

    Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    NLS-MBP-rATOM(WDR5)
    

    Tags

    NLS-MBP

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MLKS2ec

    Plasmid

    #130855

    Purpose

    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

    Insert

    MLKS2
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Available Since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. MLKS2exp

    Plasmid

    #130856

    Purpose

    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

    Insert

    MLKS2
    

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Available Since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pDGE1177

    Plasmid

    #202775

    Purpose

    CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:TREX2-zCas9_tNbEU
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. XLone-GvpB

    Plasmid

    #173798

    Purpose

    Mammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)

    Depositor

    Xiaojun Lian

    Article

    Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

    Insert

    GvpB
    

    Tags

    mCherry (K187N -please see depositor comments)

    Expression

    Mammalian

    Promoter

    TRE3G promoter

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. H5_fold-0_Elsa

    Plasmid

    #201825

    Purpose

    Expresses H5_fold-0_Elsa in bacterial cells

    Depositor

    Nobuyasu Koga

    Article

    Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

    Insert

    H5_fold-0_Elsa
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMOD_C2911

    Plasmid

    #161764

    Purpose

    MODULE C plasmid compatible with the Voytas Plant Genome Engineering Tool kit with an Agrobacterium promoter rolD expressing the TREX2 exonuclease.

    Depositor

    Shaun Curtin

    Article

    Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

    Insert

    rolD:TREX2
    

    Expression

    Bacterial

    Promoter

    rolD

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. BPTI-21

    Plasmid

    #195058

    Purpose

    simplyfied amino acid sequence by alanine scanning

    Depositor

    Peter Kim, Yutaka Kuroda

    Article

    Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. d

    Insert

    bovine pancreatic trypsin inhibitor
    

    Tags

    His tag and trpΔLE leader sequences

    Expression

    Bacterial

    Mutation

    21 amino acids are Alanine out of 58 amino acids

    Promoter

    T7 promotor

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. NAT-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid

    #193314

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4MYC N-terminal tagging
    

    Tags

    4MYC

    Expression

    Bacterial

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HYG-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid

    #193316

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4MYC N-terminal tagging
    

    Tags

    4MYC

    Expression

    Bacterial

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-28a(+)-His(6)-TEV-AtPCO5(wt)

    Plasmid

    #191803

    Purpose

    Full length wt AtPCO5 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO5
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET-28a(+)-His(6)-AtPCO1(wt)

    Plasmid

    #191798

    Purpose

    Full length wt AtPCO1 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO1
    

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-28a(+)-His(6)-AtPCO2(wt)

    Plasmid

    #191799

    Purpose

    Full length wt AtPCO2 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO2
    

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET-28a(+)-His(6)-AtPCO3(wt)

    Plasmid

    #191800

    Purpose

    Full length wt AtPCO3 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO3
    

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PRPF4.001.169

    Plasmid

    #137233

    Purpose

    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.

    Depositor

    Tara Davis

    Article

    Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

    Insert

    PRPF4 (PRPF4 Human)
    

    Tags

    (His)6-TEV

    Expression

    Bacterial

    Mutation

    001-169

    Available Since

    Nov. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLY171

    Plasmid

    #184145

    Purpose

    sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    sgRNA-LEA2-ex3
    

    Use

    Synthetic Biology

    Promoter

    Plux2

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p663-UBC-miniTurbo-V5-HIPK4_IDG-K

    Plasmid

    #158185

    Purpose

    Gateway destination clone of HIPK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-HIPK4 (HIPK4 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    A670T, H813Y- please see depositor comment

    Promoter

    Ubiquitin

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. FnRPR9-10

    Plasmid

    #182568

    Purpose

    Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 9-10
    

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1374A, P1376A, R1379A

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. FnRPR8-10

    Plasmid

    #182570

    Purpose

    Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 8-10
    

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1374A, P1376A, R1379A

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. mouse KANK4-Y801H Ankyrin Repeats pET151

    Plasmid

    #164058

    Purpose

    Expresses mouse KANK4 Ank domain -Y801H mutant in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBEL2114

    Plasmid

    #175961

    Purpose

    For expression of LetA-[ΔRing6 LetB] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Inserts

    LetA
    LetB
    

    Tags

    8xHis 2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Mutation

    Δ634-746

    Promoter

    araBAD

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. pElectra1-LaminA

    Plasmid

    #179482

    Purpose

    Nuclear envelope labeling with blue fluorescent protein Electra1

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra1-LaminA
    

    Tags

    Electra1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCEP4_HA-Halo-cmyc-GRM3 C127A

    Plasmid

    #140458

    Purpose

    Mammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127A

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Tags

    Halo, Signal/leader sequence from influenza hemag…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, Threonine 174 to alanine

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pTS2408-Tier1-PhCMV-TEVsite

    Plasmid

    #169503

    Purpose

    Tier-1 vector encoding PhCMV-controlled tabacco etch virus protease recognition site (PhCMV-TEVsite-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven tobacco etch virus protease cleavage site
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pTS2409-Tier1-PhCMV-FURINsite

    Plasmid

    #169488

    Purpose

    Tier-1 vector encoding PhCMV-controlled FURIN protease recognition site (PhCMV-FURINsite-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Furin cleavage site
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. CTAG_linker-N7

    Plasmid

    #136102

    Purpose

    Nuclear targeting peptide for C-term fusion with CDS12 without linker.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_AlaSer4xAla_N7
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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50. CTAG_NLS-SV40

    Plasmid

    #136108

    Purpose

    CTAG Nuclear localiation signal SV40 for fusion with CDS12

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_NLS-SV40
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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