We narrowed to 704 results for: Tk1

1. pKM-U6-reRNA-hsHEKsite_1_3

   Plasmid

   #205638

   Purpose

   reRNA guide targeting HEKsite_1_3

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-HEKsite_1_3 (EPHA3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRC/CMV-TYK2V678F-VSV

   Plasmid

   #139349

   Purpose

   Expression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)

   Depositor

   Sandra Pellegrini

   Article

   Gakovic et al J Biol Chem. 2008 Jul 4;283(27):18522-9.

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   V362F-V678F

   Promoter

   CMV

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AURKA_HUMAN_D0

   Plasmid

   #79739

   Purpose

   This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pAuroraA GFP-AURKA-GFP

   Plasmid

   #157765

   Purpose

   Expression of AURKA fused to GFP at both ends in mammalian cells under AurkA promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   promoter CMV replaced by AurkA promoter

   Promoter

   AURKA minimal

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CDK14 gRNA (BRDN0001147002)

   Plasmid

   #76638

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK14

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK14 (CDK14 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CDK14 gRNA (BRDN0001146521)

   Plasmid

   #76639

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK14

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK14 (CDK14 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CDK14 gRNA (BRDN0001148240)

   Plasmid

   #76640

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK14

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK14 (CDK14 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-Flt3-Y591F/Y919F

   Plasmid

   #74497

   Purpose

   Overexpression of FLT3 Y591F/Y919F mutant in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y591F/Y919F, T227M

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pMSCV-Flt3-Y591F/Y919F

   Plasmid

   #74498

   Purpose

   Retroviral expression of FLT3 Y591F/Y919F

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Y591F/Y919F, T227M

   Promoter

   unknown

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

10. JAK2 gRNA (BRDN0001149125)

    Plasmid

    #75728

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human JAK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    JAK2 (JAK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CDK16_HUMAN_D0

    Plasmid

    #79696

    Purpose

    This plasmid encodes the kinase domain of CDK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CDK16 (CDK16 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. JAK2 gRNA (BRDN0001146541)

    Plasmid

    #75726

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human JAK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    JAK2 (JAK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. JAK2 gRNA (BRDN0001147052)

    Plasmid

    #75727

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human JAK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    JAK2 (JAK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP N1 mitoAURKA

    Plasmid

    #157754

    Purpose

    Expression of AURKA:GFP targeted to mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    AURKA without the first 30 amino acids replaced b…

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. RPS6KB1 gRNA (BRDN0001145729)

    Plasmid

    #75612

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KB1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KB1 (RPS6KB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RPS6KB1 gRNA (BRDN0001145927)

    Plasmid

    #75613

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KB1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KB1 (RPS6KB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RPS6KB1 gRNA (BRDN0001148368)

    Plasmid

    #75614

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KB1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KB1 (RPS6KB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV ShadowG-AURKA-mTurq2

    Plasmid

    #157768

    Purpose

    Expression of AuroraA kinase biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    mTurquoise2 and shadowG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223-ZNF175

    Plasmid

    #88551

    Purpose

    Donor Vector containing ZNF175 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF175 (ZNF175 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EPHA3_HUMAN_D0

    Plasmid

    #79708

    Purpose

    This plasmid encodes the kinase domain of EPHA3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only