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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

Plasmid

#129053

Purpose

targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA1 (mouseTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

Plasmid

#129029

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA1 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-bio-myc-mCE 2-210

Plasmid

#112715

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBKS_WT_V5

Plasmid

#82959

Purpose

Gateway Donor vector containing RBKS, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBKS (RBKS Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAP2_WT_V5

Plasmid

#83000

Purpose

Gateway Donor vector containing STAP2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STAP2 (STAP2 Human)

Use

Gateway Cloning

Mutation

D93N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPOX_WT_V5

Plasmid

#82951

Purpose

Gateway Donor vector containing PPOX, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PPOX (PPOX Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAB31_WT_V5

Plasmid

#82993

Purpose

Gateway Donor vector containing RAB31, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RAB31 (RAB31 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRubiG-T2A-AKT3-T2A-Cre

Plasmid

#189008

Purpose

Retroviral vector with a ubiquitin promoter expressing GFP, human AKT3 transcript variant 1 ORF and Cre recombinase, separated by T2A motifs

Depositor

Bryan Luikart

Article

Prina et al Brain. 2026 Mar 9:awag098. doi: 10.1093/

Insert

AKT3 (AKT3 Human)

Use

Retroviral

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pRubiG-T2A-AKT1-T2A-Cre

Plasmid

#189011

Purpose

Retroviral vector with a ubiquitin promoter expressing GFP, human AKT1 ORF, and Cre recombinase, separated by T2A motifs.

Depositor

Bryan Luikart

Article

Prina et al Brain. 2026 Mar 9:awag098. doi: 10.1093/

Insert

AKT1 (AKT1 Human)

Use

Retroviral

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAP-Tag5-IgKsp-mHevin∆DE-Myc-His

Plasmid

#249177

Purpose

Overexpression of mouse Sparcl1 with deletion of a.a. 330-550 with Myc and 6xHis tag

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Deleted amino acids 330–550

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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