We narrowed to 14,741 results for: NTS;

1. pGWB523

   Plasmid

   #74865

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGWB507

   Plasmid

   #74849

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDGE667

   Plasmid

   #153232

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   GFP

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFH6_new

   Plasmid

   #105866

   Purpose

   more efficient version of pFH6; Subcloning of any sgRNA via BbsI site

   Depositor

   Andreas Weber

   Article

   Hahn et al Bio Protoc. 2017 Jul 5;7(13):e2384. doi:

   Insert

   U6-26p::sgRNA scaffold
   

   Use

   CRISPR and Synthetic Biology; Subcloning

   Expression

   Plant

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-FLAG-SpCas9-HF1

   Plasmid

   #126770

   Purpose

   Expression of increased fidelity SpCas9-HF1 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Pb459-mU6-sgRNA-EF1a-PuroR

   Plasmid

   #195507

   Purpose

   piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

   Depositor

   Joanna Wysocka

   Article

   Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

   Insert

   Puromycin
   

   Use

   CRISPR; Piggybac

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGWB423

   Plasmid

   #74817

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TDP43 NOTAG6

   Plasmid

   #28209

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   aa1-314 only

   Available Since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pRS303 Gal TDP-43 YFP

   Plasmid

   #27468

   Depositor

   Aaron Gitler

   Article

   Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   D23A mutation in TDP-43 sequence

   Available Since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pUb dRxCas13d + RxCas13d guide RNA

    Plasmid

    #176304

    Purpose

    Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    RxCas13d
    

    Tags

    HA tag

    Expression

    Insect

    Mutation

    R239A, H244A, R858A, H863A

    Promoter

    Ubi-p63e

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

    Plasmid

    #197952

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pcoCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6-26 and pUBQ10

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAT-9218

    Plasmid

    #124225

    Purpose

    Bacterial SpCas9 expression

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    SpCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TetR/TetA

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid

    #160139

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBabe-PI(WT)-Src(YF)-mCherry

    Plasmid

    #87357

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src isoform 2 (Src Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Y535F

    Promoter

    CMV

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSUPER IKKalpha

    Plasmid

    #26208

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    pSUPER IKK alpha (CHUK Human)
    

    Use

    RNAi

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

16. (326) pAAV SV40-ZsGreen U6-gRNA

    Plasmid

    #163020

    Purpose

    Fluorescent reporter for Sa gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Zs Green
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-BE4-RrA3F F130L

    Plasmid

    #138341

    Purpose

    Mammalian expression plasmid for BE4-RrA3F F130L

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-RrA3F F130L
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEE531

    Plasmid

    #149290

    Purpose

    T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GW1-mito-mCherryEA

    Plasmid

    #207868

    Purpose

    Mitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

    Depositor

    Mathew Tantama

    Article

    Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

    Insert

    mito-mCherryEA
    

    Tags

    4xCox8 mitochondrial targeting

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMP71-tCD19-LLO-SIINFEKL

    Plasmid

    #174598

    Purpose

    expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and SIINFEKL epitopes

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    membrane bound CD19, LLO antigen, SIINFEKL (ovalbumin) antigen
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge