We narrowed to 14,741 results for: NTS;

1. pCDNA5/FRT/TO_H2B_HaloTag9

   Plasmid

   #169334

   Purpose

   Nuclear expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag9 (H2BC21 Human)
   

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-NtLAtC60αβC20

   Plasmid

   #160873

   Purpose

   Expression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcL, cpn60α, cpn60β, cpn20
   

   Expression

   Bacterial

   Promoter

   rbcL: pBAD, chaperones: T7

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGE670

   Plasmid

   #153236

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE463

   Plasmid

   #153234

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Erk1

   Plasmid

   #29578

   Depositor

   Dustin Maly

   Article

   Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

   Insert

   Erk1 (MAPK3 Human)
   

   Tags

   FLAG, HIS6, and TEV

   Expression

   Bacterial

   Mutation

   K289N mutation present. Starting Met removed from…

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pCA15-Ubc-NLS- scFV-tdTomato

   Plasmid

   #199446

   Purpose

   Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells

   Depositor

   Baohui Chen

   Article

   Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

   Insert

   scFV-tdTomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   human ubiquitin C (UBC)

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1

   Plasmid

   #46676

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-mir-133a-1 (MIR133A1 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro

   Plasmid

   #31480

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (ani target)
   

   Use

   Lentiviral

   Mutation

   mCherry has M9S, M16L to reduce background mCherr…

   Available Since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFAB815

   Plasmid

   #47854

   Purpose

   BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   rnpB T1 terminator
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLTetO9

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTX209

    Plasmid

    #89269

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsPDS-gRNA01 and OsPDS-gRNA02
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Promoter

    Maize Ubiquitin1 promoter

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GalT-moxDendra2

    Plasmid

    #89789

    Purpose

    mammalian expression of GC localized moxDendra2

    Depositor

    Erik Snapp

    Article

    Kaberniuk et al Chem Commun (Camb). 2017 Feb 9;53(13):21

    Insert

    GalT signal anchor transmembrane domain (B4GALT1 Human)
    

    Tags

    moxDendra2

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB421

    Plasmid

    #74815

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. KHBD00222

    Plasmid

    #34370

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1832 (CG1832 Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pMOD_A0402

    Plasmid

    #91009

    Purpose

    Module A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Mutation

    D10A, H840A

    Promoter

    AtUbi10

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGE331

    Plasmid

    #153240

    Purpose

    M1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDECKO_TFRC_B

    Plasmid

    #72626

    Purpose

    Expresses two gRNAs targeting the TFRC promoter

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward TFRC
    

    Expression

    Mammalian

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-PI(WT)-Src(KD)-mCherry

    Plasmid

    #87358

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src (Src Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    D388R

    Promoter

    CMV

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL2-200bp

    Plasmid

    #16293

    Depositor

    Wafik El-Deiry

    Article

    Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

    Insert

    p53 promoter fragment (TP53 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    fragment -188 to +12 relative to first transcript…

    Available Since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pGWB524

    Plasmid

    #74866

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-HA-ERK2 L198A L232A

    Plasmid

    #8978

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L198A, L232A

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only