We narrowed to 9,677 results for: bli

1. p-dCas9-G9a-Hygro

   Plasmid

   #104408

   Purpose

   transient expression of dCas9-G9a fusion protein

   Depositor

   Stephan Beck

   Insert

   G9a (EHMT2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LGmCreER (self deleting)

   Plasmid

   #33340

   Depositor

   Scott Lowe

   Article

   Zhao et al Genes Dev. 2010 Jul 1;24(13):1389-402.

   Insert

   CreERT2
   

   Use

   Cre/Lox and Retroviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAMK2A

   Plasmid

   #38971

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CAMK2A (CAMK2A Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pFRT-TODestGFP_HuD

   Plasmid

   #65763

   Purpose

   expresses GFP-tagged HuD in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL4 (ELAVL4 Human)
   

   Tags

   FLAG HA and V5

   Expression

   Mammalian

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiGuide-Puro PRMT5-CRISPRi

   Plasmid

   #164637

   Purpose

   lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

   Depositor

   Günter Schneider

   Insert

   target guide sequence PRMT5 inhibition (PRMT5 Human)
   

   Use

   CRISPR and Lentiviral; Interference

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hs.HRAS C186S

   Plasmid

   #83189

   Purpose

   Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), C186S mutation

   Depositor

   Dominic Esposito

   Insert

   HRAS (HRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   C186S

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Sac-Abeta(MC1-40)

   Plasmid

   #127152

   Purpose

   Expresses N-terminal cysteine Abeta(1-40)

   Depositor

   James Nowick

   Insert

   Abeta(MC1-40) (APP Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. XLone-BSD SARS-CoV2 N P2A mCherry

   Plasmid

   #154398

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry

   Depositor

   Xiaoping Bao

   Insert

   SARS-CoV2 N Protein (N SARS-CoV2)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. RHPMP132 (mEOS3.1-beta Actin)

   Plasmid

   #88836

   Purpose

   Localization by Super-Resolution microscopy of beta-Actin fused to mEOS3.1

   Depositor

   Ricardo Henriques

   Insert

   beta-Actin (ACTB Human)
   

   Tags

   mEOS3.1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFRT-TODestRFP_HuD

    Plasmid

    #65766

    Purpose

    expresses RFP-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Expression

    Mammalian

    Mutation

    V23M mutation in RFP

    Promoter

    CMV, 2x Tet

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pDONR221-APN

    Plasmid

    #158440

    Purpose

    Gateway Cloning compatible entry vector for the human APN gene.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    APN (ANPEP Human)
    

    Use

    Gateway entry vector

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-Flag-Mm Ezh2 Y641F

    Plasmid

    #80077

    Purpose

    Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection

    Depositor

    Phillip Abbosh

    Inserts

    Flag-EZH2 Y641F (Ezh2 Mouse)
    Hygromycin phosphotransferase
    

    Use

    Retroviral

    Tags

    5' FLAG

    Expression

    Mammalian

    Mutation

    Y641F, R342R, N393N

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. RHPMP133 (mOrange2-beta Actin)

    Plasmid

    #88837

    Purpose

    Localization by Super-Resolution microscopy of beta-Actin fused to mOrange2

    Depositor

    Ricardo Henriques

    Insert

    beta-Actin (ACTB Human)
    

    Tags

    mOrange2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRT-DestFLAGHA_HuB

    Plasmid

    #65758

    Purpose

    constitutively expresses FLAG-HA-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

15. GPX3

    Plasmid

    #38899

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GPX3 (GPX3 Human)
    

    Tags

    Tev His Flag

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pFRT-TODestGFP_HuB

    Plasmid

    #65761

    Purpose

    expresses GFP-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-UbC-eGFP-F

    Plasmid

    #71545

    Purpose

    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.

    Depositor

    Pantelis Tsoulfas

    Insert

    eGFP-F
    

    Use

    AAV

    Tags

    contains 20- amino-acid farnesylation signal from…

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. tol2-4xnr UAS-PA Rac1-mCherry

    Plasmid

    #41878

    Depositor

    Anna Huttenlocher

    Insert

    photoactivatable Rac1 (RAC1 Human)
    

    Use

    Zebrafish expression

    Tags

    6x His and mCherry

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNIC28-hKdm2b-CxxC-PHD

    Plasmid

    #49216

    Purpose

    Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.

    Depositor

    Rob Klose

    Insert

    Kdm2b (KDM2B Human)
    

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. COVID-SARS2 NSP16/10

    Plasmid

    #159615

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10

    Depositor

    Opher Gileadi

    Inserts

    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    

    Tags

    His6-Zb-TEV and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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