We narrowed to 9,677 results for: bli

1. pAAV Syn 2xLyn-ERex-mKate2-bPAC(F198Y)

   Plasmid

   #219654

   Purpose

   Neuronal expression of PACmn with mKate2, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity.

   Depositor

   Thomas Oertner

   Insert

   2xLyn-ERex-mKate2-bPAC(F198Y)
   

   Use

   AAV

   Tags

   ER exit ( FCYENE) and Lyn11 (GCIKSKGKDS)

   Expression

   Mammalian

   Promoter

   Syn

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ACE2 g3

   Plasmid

   #153013

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

   Depositor

   Jason Sheltzer

   Insert

   ACE2 (ACE2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MARS

   Plasmid

   #205232

   Purpose

   Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells

   Depositor

   Jeremy Baskin

   Insert

   PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
   

   Tags

   Nuclear Export Sequence and mScarlet-i

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. K560GFP_17NL

   Plasmid

   #129767

   Purpose

   Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Tags

   GFP and His6

   Expression

   Bacterial

   Mutation

   DAL insert before A345

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-L3US2-RFP-HDAC3

   Plasmid

   #83966

   Purpose

   Lentiviral CRISPR HDAC3 dual gRNA targeting vector

   Depositor

   Eleanor Chen

   Insert

   HDAC3 dual gRNAs (HDAC3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pES133

   Plasmid

   #127552

   Purpose

   Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13

   Depositor

   Emmanuele Severi, Gavin Thomas

   Insert

   F5-flanked tetracycline resistance cassette
   

   Expression

   Bacterial

   Mutation

   Multiple SNPs in tetA (originally from pBR322) to…

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG416-GAL-hnRNPA1

   Plasmid

   #181710

   Purpose

   Galactose inducible expression of hnRNPA1

   Depositor

   Alejandro Chavez

   Insert

   hnRNPA1 (HNRNPA1 Human)
   

   Expression

   Yeast

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. tet-pLKO.puro_shHuR CDS

   Plasmid

   #110411

   Purpose

   TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAVL1 (HuR)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1/TO (RNA PolIII)

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. HPGD

   Plasmid

   #39154

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HPGD (HPGD Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. tet-pLKO.puro_shGAC

    Plasmid

    #110423

    Purpose

    shGAC, silence glutaminase GAC isoform, doxycycline inducible, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1/TO (RNA PolIII)

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. isoform PKC Iota (JCE597)

    Plasmid

    #182030

    Purpose

    yeast expression of mouse PKC Iota with yeast PKC1 promoter and ADH1 terminator

    Depositor

    Juan Carlos Igual

    Insert

    PKC Iota (Prkci Mouse)
    

    Expression

    Mammalian

    Promoter

    yeast PKC1 promoter (-600 to -1)

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX307-CTSB-G418

    Plasmid

    #158465

    Purpose

    Constitutive lentiviral expression of human CTSB.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    CTSB (CTSB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHN873

    Plasmid

    #178829

    Purpose

    Bacterial expression for human Saposin A

    Depositor

    Kazuhiro Abe

    Insert

    artificial gene encoding human Saposin A (AR Human)
    

    Tags

    MBP, His8, TEV

    Expression

    Bacterial

    Mutation

    Codon-usage was optimized for bacterial expressio…

    Promoter

    T7

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. GOT1

    Plasmid

    #39022

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GOT1 (GOT1 Human)
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. Hs.HRAS Q61L

    Plasmid

    #83186

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61L mutation

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61L

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG416-GAL-TDP-43

    Plasmid

    #181709

    Purpose

    Galactose inducible expression of TDP-43

    Depositor

    Alejandro Chavez

    Insert

    TDP-43 (TARDBP Human)
    

    Expression

    Yeast

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIVT-myc-T58A-S62D

    Plasmid

    #138540

    Purpose

    For making myc-T58A-S62D RNA

    Depositor

    Carmen Williams

    Insert

    Myelocytomatosis oncogene T58A S62D (Myc Mouse)
    

    Use

    In vitro transcription

    Mutation

    Threonine 58 changed to alanine and serine 62 cha…

    Promoter

    T7

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-tkd2MetGFP

    Plasmid

    #37562

    Depositor

    David Rimm

    Insert

    MET (MET Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    only contains cytoplasmic domain amino acids L115…

    Promoter

    CMV

    Available Since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMAL-c5X-Darpin E_01

    Plasmid

    #66996

    Purpose

    Expresses malE-Darpin E_01 in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-Darpin E_01
    

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. CBLB

    Plasmid

    #39056

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CBLB (CBLB Human)
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only