We narrowed to 9,668 results for: bli

1. pALS3-Ma-sfGFP-TAG150

   Plasmid

   #212121

   Purpose

   Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

   Depositor

   Ryan Mehl

   Inserts

   sfGFP-150TAG
   M. alvus Pyl-tRNA(6)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   N150TAG

   Promoter

   araC and lpp

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK-Ma pylRS nitroY/haloY-F5

   Plasmid

   #212125

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.

   Depositor

   Ryan Mehl

   Insert

   M. alvus PylRS 3-NY/HaloY F5
   

   Expression

   Bacterial

   Mutation

   CTG125CTT, N166A V168S, A223C, W239R

   Promoter

   GlnS (constitutive)

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AICSDP-117:POLR2A-mEGFP

   Plasmid

   #164500

   Purpose

   Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

   Depositor

   Allen Institute for Cell Science

   Insert

   POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Feb. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. psd44-G12QL

   Plasmid

   #46825

   Purpose

   expression of mutant G protein alpha 12 in mammalian cells

   Depositor

   Agnese Mariotti

   Insert

   GNA12 (GNA12 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Q231L (the mutation reduces GTPase activity resul…

   Promoter

   PGK

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG416-GAL-FUS-G156E

   Plasmid

   #181716

   Purpose

   Galactose inducible expression of FUS-G156E

   Depositor

   Alejandro Chavez

   Insert

   FUS (FUS Human)
   

   Expression

   Yeast

   Mutation

   G156E

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hs.KRAS4a

   Plasmid

   #83166

   Purpose

   Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine

   Plasmid

   #50467

   Purpose

   Gi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoter

   Depositor

   Bryan Roth

   Has Service

   AAV2 and AAV8

   Insert

   hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   CaMKIIa

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine

   Plasmid

   #50471

   Purpose

   Gi-coupled hM4D-IRES-mCitrine under the control of GFAP promoter

   Depositor

   Bryan Roth

   Insert

   hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   GFAP

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJCX3-ACTG2_R257C

   Plasmid

   #228911

   Purpose

   Expresses mutant R257C smooth muscle actin gamma-2 in human cells

   Depositor

   Roberto Dominguez

   Insert

   Actin gamma 2, smooth muscle (ACTG2 Human)
   

   Tags

   HIS-FLAG-TEV

   Expression

   Bacterial and Mammalian

   Mutation

   R257C

   Promoter

   CMV

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJCX3-ACTG2_WT

    Plasmid

    #228910

    Purpose

    Expresses wildtype smooth muscle actin gamma-2 in human cells

    Depositor

    Roberto Dominguez

    Insert

    Actin gamma 2, smooth muscle (ACTG2 Human)
    

    Tags

    HIS-FLAG-TEV

    Expression

    Bacterial and Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6xO/S luc

    Plasmid

    #69445

    Purpose

    detection/assessment of OCT4 and SOX2 transcription factor activity

    Depositor

    Lisa Dailey

    Insert

    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Hs.KRAS4b G13D

    Plasmid

    #83133

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13D mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G13D

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. ACACA

    Plasmid

    #39083

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ACACA (ACACA Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. SARS-CoV-2 Spike-d18

    Plasmid

    #164583

    Purpose

    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

    Depositor

    Jason Sheltzer

    Insert

    SARS-CoV-2 Spike (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1256-1273

    Promoter

    EF1a

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lamin A (G465D)-mRFP

    Plasmid

    #124271

    Purpose

    Lamin A mutant (G465D), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.

    Depositor

    Eric Schirmer

    Insert

    LMNA (G465D) (LMNA Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    LMNA (G465D)

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. EEF1A2 in pENTR

    Plasmid

    #216585

    Purpose

    gateway cloning donor vector containing EEF1A2

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    EEF1A2 (EEF1A2 Human)
    

    Use

    Gateway entry plasmid

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOET5 CVB3-VLP

    Plasmid

    #218146

    Purpose

    Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).

    Depositor

    Minna Hankaniemi

    Inserts

    VP1–VP4
    3CD
    

    Expression

    Insect

    Promoter

    CMV and Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWB2-CYCD3;1

    Plasmid

    #140750

    Purpose

    Inducing plant cells to enter mitosis

    Depositor

    Bo Liu

    Article

    Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

    Insert

    Cyclin D3;1 (CYCD3;1 Mustard Weed)
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLPS-hSox2-gfp

    Plasmid

    #49390

    Purpose

    mammalian expression of human Sox2 fused to EGFP

    Depositor

    Randall McKinnon

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    TGA (stop) to CCA (Pro) at nt 1027 with respect t…

    Promoter

    CMV

    Available Since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. Hs.KRAS4b G12C

    Plasmid

    #83130

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12C mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12C

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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