We narrowed to 9,668 results for: bli

1. pAAV-TREtight-mTagBFP2-N2cG

   Plasmid

   #192838

   Purpose

   plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

   Depositor

   Ian Wickersham

   Insert

   mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TREtight

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE-T2-IRES-His6-Ubiquitin

   Plasmid

   #107107

   Purpose

   Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.

   Depositor

   Michael E. Wright

   Insert

   human ubiquitin UBB (UBB Human)
   

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   Tet

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PE-SUMO CRYAB

   Plasmid

   #217670

   Purpose

   For expression of SUMO tagged human alpha B crystallin

   Depositor

   Larry David

   Insert

   CRYAB (CRYAB Human)
   

   Tags

   6XHis and Smt3

   Expression

   Bacterial

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201151

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   SadCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Mutation

   gRNA sequence: ATCAGTGATAGAGAACGTATG

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD_IPF2.0

   Plasmid

   #163124

   Purpose

   A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

   Depositor

   Julie Biteen

   Article

   Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

   Insert

   IFP2.0
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAMK2G

   Plasmid

   #38894

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CAMK2G (CAMK2G Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pRSET-His-APEX2

   Plasmid

   #201471

   Purpose

   Recombinant soluble APEX2 for SLAPSHOT labeling

   Depositor

   Yuh-Nung Jan

   Article

   Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

   Insert

   APEX2
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV2-CAGIG-LAMP1-mGL

   Plasmid

   #222394

   Purpose

   To express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.

   Depositor

   Pantelis Tsoulfas

   Insert

   LAMP1 and mGreenlantern (LAMP1 Synthetic, Human, Homo Sapiens)
   

   Use

   AAV

   Tags

   LAMP1 and mGreenLantern

   Expression

   Mammalian

   Promoter

   CAG or CAGIG

   Available Since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

   Plasmid

   #159912

   Purpose

   Mutagenesis of Kcnd3

   Depositor

   Larry Zweifel

   Insert

   Kcnd3 (Kcnd3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

    Plasmid

    #105715

    Purpose

    In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

    Depositor

    Rylan Larsen

    Has Service

    AAV5

    Insert

    synaptophysin-GCaMP6s
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG426-GAL-Alpha-synuclein-H50Q

    Plasmid

    #181724

    Purpose

    Galactose inducible expression of Alpha-synuclein-H50Q

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Expression

    Yeast

    Mutation

    H50Q

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pScDD2_ERdd

    Plasmid

    #109047

    Purpose

    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).

    Depositor

    Thomas Wandless

    Insert

    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    

    Tags

    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…

    Expression

    Yeast

    Mutation

    3 mutations : E380G, R434G, N532S

    Promoter

    SOD1

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CD44Z-c011

    Plasmid

    #175455

    Purpose

    Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Tags

    His6

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-blast shKGA

    Plasmid

    #110420

    Purpose

    shKGA, silence glutaminase KGA isoform, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 (RNA Pol III)

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-dCas9-p300-Hygro

    Plasmid

    #104411

    Purpose

    transient expression of dCas9-p300 fusion protein

    Depositor

    Stephan Beck

    Insert

    p300 (EP300 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTP-AAV-ChATen-mCherry

    Plasmid

    #193741

    Purpose

    This AAV vector contains a ChAT enhancer that drives mCherry expression in spinal cord skeletal motor neurons. Specificity of vector not characterized outside the mouse spinal cord.

    Depositor

    Hynek Wichterle

    Insert

    ChAT enhancer, mini promoter, synthetic intron, mCherry
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Promoter

    1000 bp ChAT enhancer with a 32 bp minimal promot…

    Available Since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEC2735 (pSpy0C2)

    Plasmid

    #220279

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. DOT1L

    Plasmid

    #36196

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (GeneScript Synthesized) (DOT1L Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acid residues 1-420

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

19. saposin B^W

    Plasmid

    #161759

    Purpose

    Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

    Depositor

    Sam Gellman

    Article

    Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

    Insert

    saposin B R38_W
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    changed Arginine 38 to Tryptophan

    Promoter

    T7

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. DOT1L

    Plasmid

    #40736

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (DOT1L Human)
    

    Tags

    6xHis TEV

    Expression

    Bacterial

    Available Since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits