We narrowed to 2,079 results for: membrane gfp
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Plasmid#26867PurposeExpresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membraneDepositorInsertCIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
TagsEGFPExpressionMammalianMutationNLS sequences mutatedPromoterCMVAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
Arch(D95H)-eGFP
Plasmid#51081PurposeExpresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltageDepositorInsertArchaerhodopsin-3 w/ D95H point mutation fused to eGFP
UseLentiviralTagseGFPExpressionMammalianMutationChanged Aspartic Acid 95 to HistidinePromoterUbiquitinAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
LVDP 2P.2.EGFP
Plasmid#231905PurposeFor the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging EGFP mRNA into the VLPsDepositorUseLentiviralMutationR203M in N proteinPromoterCMV, EF-1aAvailable SinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-GFP
Plasmid#50486Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
TagsGFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the GFP open…PromoterCMVAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- WTN23C11orf83-GFP
Plasmid#65845PurposeMammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorInsertTransmembrane (23 AA) of UQCC3 (UQCC3 Human)
TagsGFPExpressionMammalianMutationWT TransmembraneAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET_GFP-SZ2
Plasmid#124931PurposeExpression of GFP-SZ2DepositorInsertGFP-SYNZIP2
TagsGFP and HisExpressionBacterialMutationSZ2 domainAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-GFP
Plasmid#166840PurposeExpresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorTagsGFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin
Plasmid#134661PurposeExpresses EGFP-tagged synaptic vesicle proteinDepositorInsertvesicle-associated membrane protein 2 (VAMP2 Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-myr-EGFP-LDLR(Ct)
Plasmid#213402Purposedouple floxed, myristoylation (myr) site for membrane targeting and EGFPDepositorInsertEGFP
UseAAVTagsC-terminal (Ct) cytoplasmic domains of low densit…ExpressionMammalianAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SP-GFP-CD274-NU
Plasmid#121139PurposeMammalian expression of GFP tagged CD274DepositorAvailable SinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-
Plasmid#115516PurposePlasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporterDepositorInsertMLS-KillerRed
UseGateway CloningTagsMLSPromoterUASAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bet1-GFP
Plasmid#209850PurposeExpresses Bet1-GFP in mammalian cellsDepositorAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP-HRP-TM_pLX304
Plasmid#202021Purposeexpresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vectorDepositorInsertFlag-GFP-HRP-TM
UseLentiviralTagsFlag and GFPExpressionMammalianPromoterCMVAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynLAMA-F98 IRES EGFP
Plasmid#130710PurposePlasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertLyn11-SNAP-Tag-LAMA-F98 IRES EGFP
TagsLyn11 and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-myr-CSK-GFP
Plasmid#83470PurposeLentiviral vector for doxycycline-inducible expression of membrane targeted-CSK in mammalian cellsDepositorInsertCSK (CSK Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterdoxycycline-inducible promoterAvailable SinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mGFP-LLO-mPMEL
Plasmid#174608Purposeexpresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the murine hPMEL epitopeDepositorInsertmembrane GFP fused to LLO and mouse PMEL antigen
ExpressionMammalianAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynLAMA-G97 IRES EGFP
Plasmid#130711PurposePlasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertLyn11-SNAP-Tag-LAMA-G97 IRES EGFP
TagsLyn11 and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Xl-VSP1 R152Q GFP
Plasmid#51884Purposeexpression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cellsDepositorInsertVSP1 R152Q (tpte2 Frog)
UseXenopus oocyte expressionTagsEGFPExpressionMammalianMutationR152Q: near the extracellular side of the fourth …PromoterCMVAvailable SinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFP-ST-P2A-pSC-CAAX
Plasmid#207637PurposeA plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.DepositorInsertEGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
ExpressionMammalianMutationAmber stop codon at SC's critical lysinePromoterCMVAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-RHD272-472-GFP
Plasmid#186606PurposeEncodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFPDepositorInsertHuman Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
TagsmNeonGreenExpressionMammalianMutationMet-Gly-Ser-Lys peptide added at N-terminus for c…PromoterCMVAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRESh-GFPII-MB-Dlk1
Plasmid#108930PurposeGFP-tagged vector expressing membrane-bound Dlk1DepositorInsertDlk1 (Dlk1 Mouse)
TagsGFPIIExpressionMammalianMutationlacks coding sequence for the juxtamembrane motif…Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenSyn2-EGFP-OMP25C
Plasmid#71427PurposeLentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.DepositorInsertOMP25 (172-‐206) (Synj2bp Rat)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 172-206 onlyPromotersynapsinAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-acVHH (Y34W)-PB
Plasmid#171072PurposeA COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
Tagsmodified STIM1–PB tagExpressionMammalianMutationY34WPromoterCMVAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-acVHH (Y34F)-PB
Plasmid#171071PurposeA COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
Tagsmodified STIM1–PB tagExpressionMammalianMutationY34FPromoterCMVAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP–acVHH (Y104F)–PB
Plasmid#169683PurposeA COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
Tagsmodified STIM1–PB tagExpressionMammalianMutationY104FPromoterCMVAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
paGFP-Omp25
Plasmid#69598Purposeexpresses GFP-labeled mitochondrial outer membrane protein 25DepositorInsertpaGFP-Omp25 (Synj2bp Rat)
UseLentiviralTagsGFPExpressionMammalianMutationphotoactivatable GFP fused to rat Omp25 C-terminusPromoterCMVAvailable SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP
Plasmid#58490PurposeExpresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltageDepositorInsertArchaerhodopsin-3 w/ D95H point mutation fused to eGFP
UseLentiviralTagseGFPExpressionMammalianMutationChanged Aspartic Acid 95 to GlutaminePromoterUbiquitinAvailable SinceAug. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-YPT31
Plasmid#105263PurposePlasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.DepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-iGFP-YPT52
Plasmid#105279PurposePlasmid for integration of iGFP-Ypt52 at TRP1.DepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-PIK1
Plasmid#105260PurposePlasmid for pop-in/pop-out replacement of Pik1 with iGFP-Pik1.DepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only